Skip to main content
Log in

Improving the quality of minced beef by using mulberry leaves extract

  • Original Paper
  • Published:
Journal of Food Measurement and Characterization Aims and scope Submit manuscript

Abstract

The aim of this research was to study the use of different concentrations (2, 4 and 6% w/w) of methanolic extract of mulberry leaves (Morus spp.) to increase the shelf life of minced beef. Treated samples were compared with the control and gamma irradiated sample at dose level of 5 kGy during cold storage at (4 ± 1 °C) for 24 days. Microbiological, chemical and sensory characteristics of treated minced beef were determined every 2 days during the cold storage. Obtained results showed that minced beef treated with different concentrations of mulberry leaves methanolic extract reduced the initial total bacterial count, psychrophilic bacteria, enterobacteriaceae and total mold & yeast as well as prolonged the shelf-life of minced beef compared to the control. Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis and Escherichia coli counts were reduced. The mulberry leaves methanolic extract caused slight increase in the thiobarbituric acid reactive substances and total volatile nitrogen contents of minced beef during the cold storage. Sensory results showed that the shelf life of minced beef was 6 days for the control, and 8, 10, and 14 days for minced samples treated with mulberry leaves methanolic extract (2, 4 and 6%) respectively compared to gamma irradiated (22 days).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. G.J.E. Nychas, in Natural antimicrobial from plants, ed. By G.W. Gould. New Method of Food Preservation (Chapman and Hall, Glasgow, 1995)

    Google Scholar 

  2. U.C. Kanife, O.S. Odesanmi, A.A. Adekumle, V.F. Doherty, Effects of ethanol extracts of healthy and infected Panicum maximum (Jacq.) Floret on liver and kidney function profile and histopathology in Sprague-dawley rats. Res. J. Recent Sci. 1, 8–13 (2012)

    CAS  Google Scholar 

  3. E. Flaczyk, J. Kobus Cisowska, M. Przeor, J. Korczak, M. Remiszewski, E. Korbas, M. Buchowski, Chemical characterization and oxidative properties of polish variety of Morus alba L. leaf aqueous extracts from the labrotory and pilot-scale processes. Agric. Sci. 4, 141–147 (2013)

    CAS  Google Scholar 

  4. J.Y. Yang, H.S. Lee, Evaluation of antioxidant and antibacterial activities of morin isolated mulberry fruits (Morus alba L.). J. Korean Soc. Appl. Biol. Chem. 55, 485–489 (2012)

    Article  CAS  Google Scholar 

  5. S. Anis, T. Bhargava, H. Upadhyay, A review on phytotherapy by Morus Alba. Int. J. Pharm. Chem. Sci. 1, 1563–1566 (2012)

    Google Scholar 

  6. D.A. Kostić, D. S. Dimitrijević, S.S. Mitić, M.N. Mitić, G.S. Stojanović, A.V. Zivanović, A survey on macro- and micro-elements, phenolic compounds, biological activity and use of Morus spp. (Moraceae). Fruits 68, 333–347 (2013)

    Article  Google Scholar 

  7. M.Z.M. Salem, H. Aly, Y. Gohar, A.W. El-Sayed, Biological activity of extracts from Morus alba L., Albizzia lebbeck (L.) Benth. and Casuarina glauca Sieber against the growth of some pathogenic bacteria. Int. J. Agric. Food Res. 2, 9–22 (2013)

    Google Scholar 

  8. D.E. Stevenson, R.D. Hurst, Polyphenolic phytochemicals–just antioxidants or much more. Life Sci. 64, 2900 (2007)

    CAS  Google Scholar 

  9. A. Saeedeh, U. Asna, Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves. Food Chem. 102, 1233–1240 (2007)

    Article  Google Scholar 

  10. J.D. Ordon, M.A. Gomez, M.I. Vattuone, Antioxidant activities of Sechiumedule (Jacq.) Swartz extracts. Food Chem. 97, 452–458 (2006)

    Article  Google Scholar 

  11. R.P. Singh, K.N.C. Murthy, G.K. Jayaprakasha, Studies on the antioxidant activity of pomegranate peel and seed extracts using in vitro models. J. Agric. Food Chem. 50, 81–86 (2002)

    Article  CAS  Google Scholar 

  12. N.A. Mwansyemela, Measurement of lipid peroxidation. Thiobarbaturic acid value (TBARS) 2-thiobarbituric acid (1992) http://www.sciepub.com/portal/downloads

  13. C. Alasnier, A. Meynier, M. Viau, G. Gandmer, Hydrolytic and oxidative changes in the lipids of chicken breast and thigh muscles during refrigerated storage. J. Food Sci. 65, 9–14 (2000)

    Article  CAS  Google Scholar 

  14. ICMSF International Committee on Microbiological Specifications for Foods, Microorganisms in foods, their significance and methods of enumeration. 2nd edn. (International Committee on Microbiological Specifications for Foods, University of Toronto Press, Toronto and Buffalo, 1996)

    Google Scholar 

  15. APHA, Compendium of Methods for the Microbiological Examination of Foods, 2nd ed. (American Public Health Association, Washington D.C., 1992)

    Google Scholar 

  16. D. Roberts, W. Hooper, M. Greenwood, Practical Food Microbiology; Methods for the Examination of Food for Microorganisms of Public Health Significance (Public Health Laboratory Service, London, 1995)

    Google Scholar 

  17. Oxoid, The Oxoid Manual, 8th edn. (Basingstoke, 1998)

  18. G.I. Barrow, R.K.A. Feltham, Cowan and Steel’s Manual for the identification of medical bacteria, vol. 331, 3 ed. (Cambridge University Press, Cambridge, 1993)

    Book  Google Scholar 

  19. E. Wierbicki, Food Irradiation Processing, vol. 79 (IAEA Publications, Vienna, 1985)

    Google Scholar 

  20. SAS Institute, SAS/STAT User’s Guide. (Statistical Analysis Institute, Cary, 1998).

    Google Scholar 

  21. A.A. Memon, N. Memon, D. Luthria, M.I. Bhanger, A.A. Pitaf, Phenolic acids profiling and antioxidant potential of mulberry (Morus laevigata W., Morus nigra L., Morus alba L.) leaves and fruits grown in Pakistan. Pol. J. Food Nutr. Sci. 60, 25–32 (2010)

    CAS  Google Scholar 

  22. M.M. Radojković, Z.P. Zeković, S.S. Vidović, D.D. Kočar, P.Z. Mašković, Free radical scavenging activity and total phenolic and flavonoid contents of mulberry (Morus spp. L., Moraceae) extracts. Hem. Ind. 66, 547–552 (2012)

    Article  Google Scholar 

  23. G. Paganga, N. Miller, C.A. Rice-Evans, The polyphenolic content of fruit and vegetables and their an-tioxidant activities. What does a serving constitute? Free Radic. Res. 30, 153–162 (1999)

    Article  CAS  Google Scholar 

  24. S.F. Mexis, E. Chouliara, M.G. Kontominas, Shelf life extension of ground chicken meat using an oxygen absorber and a citrus extract. LWT-Food Sci. Technol. 49, 21–27 (2012)

    Article  CAS  Google Scholar 

  25. J. Fernandez, J. Perez-Alvarez, J. Fernandez-Lopez, Thiobarbituric acid test for monitoring lipid oxidation in meat. Food Chem. 59, 345–353 (1997)

    Article  CAS  Google Scholar 

  26. S.S. Tawfik, A.I. Atia, H.M. El-Kabbany, M.H. Sallam, The use of multiple antimicrobial treatments for decontaminating meat. Egypt. J. Rad. Sci. Appl. 20, 319–336 (2007)

    Google Scholar 

  27. W.W. Nawar, Radiolytic changes in fats. Rad. Res. Rev. 3, 327 (1972)

    CAS  Google Scholar 

  28. K.W. McMillin, Initiation of oxidative processes in muscle foods. Reciprocal Meat Conference Proceedings 49 (1996)

  29. C. Ruiz-Capillas, A. Moral, Sensory and biochemical aspects of quality of whole bigeye tuna (Thunnus obesus) during bulk storage in controlled atmospheres. Food Chem. 89, 347–354 (2005)

    Article  CAS  Google Scholar 

  30. B. Andallu, A.V. Vinay Kumar, N. Varadacharyulu, Lipid abnormalities in streptozotocin-diabetes: amelioration by Morusindica L. cv Suguna leaves. Int. J. Diabetes Dev. Ctries. 29, 123–128 (2009)

    Article  CAS  Google Scholar 

  31. J. Kobus, E. Flaczyk, Comparsion of antioxidant activity water extracts from leaves Morus alba L. and Ginkgo biloba L., Pol. J. Hum. Nutr. Metab. 34, 1382–1386 (2007)

    CAS  Google Scholar 

  32. R.G. Brannan, Effect of grape seed extract on physicochemical properties of ground, salted chicken thigh meat during refrigerated storage at different relative humidity levels. Food Chem. 73, 36–38 (2008)

    Google Scholar 

  33. S.K. Devatkal, K. Narsaiah, A. Borah, Anti-oxidant effect of extracts of kinnow rind, pomegranate rind and seed powder in cooked goat meat patties. Meat Sci. 85, 155–159 (2010)

    Article  CAS  Google Scholar 

  34. B.M. Naveena, A.R. Sen, R.P. Kingsly, D.B. Singh, N. Kondaiah, Antioxidant activity of pomegranate rind powder extract in cooked chicken patties. Int. J. Food Sci. Technol. 43, 1807–1812 (2008)

    Article  CAS  Google Scholar 

  35. S.J. Aditya Rao, C.K. Ramesh, M. Riaz, B.T. Prabhakar, Anthelmintic and antimicrobial activities in some species of mulberry. Int. J. Pharm. Pharm. Sci. 4, 335–338 (2012)

    Google Scholar 

  36. C. Niratker, P. Malti, Antimicrobial activity of leaf extract of Morusindica (Mulberry) from Chhattisgarh. Asian J. Plant Sci. Res. 5, 28–31 (2015)

    Google Scholar 

  37. M.R Cleland, J. Meissner, A.S. Herer, E.W. Besrs, Adv. Rad. Chem. Polym. Proceedings of a technical meeting held in Notre Dame, Indiana, USA 13–17 September 2003, Conference Proceeding, 576, 783–786 (2004)

  38. B. Anabela, F. Carla, J.S. Maria, S. Manuel, Antibacterial activity and mode of action of ferulic and gallic acids against pathogenic bacteria. Microb Drug Resist. 00(0), 1–10 (2013)

    Google Scholar 

  39. A.H. Rady, H.H. Khalaf, H.M. Badr, M.H. Abdeldaiem, Improving the quality of ready-to-eat meals by gamma irradiation. 1-cooked meat and mashed potatoes. Egypt. J. Rad. Sci. Appl. 37, 35–54 (2005)

    Google Scholar 

  40. M.H. Abdeldaiem, Using of combined treatment between edible coatings containing ethanolic extract of papaya (Carica papaya L.) leaves and gamma irradiation for extending shelf-life of minced chicken meat. Am J. Food Sci. Technol. 2, 6–16 (2014)

    Google Scholar 

  41. E. Valdez, A. Cárdenas, C. Morales, M. Verde-Star, P. Rosales, Biological activities of Morus celtidifolia leaf extracts. Pak. J. Pharm. Sci. 28, 1177–1180 (2015)

    Google Scholar 

  42. A. Emniyet, E. Avcı, B. Ozcelik, G. Avcı, D. Kose, Antioxidant and antimicrobial activities with GC/MS analysis of the Morus alba L. leaves. Hittite J. Sci. Eng. 1, 37–41 (2014)

    Article  Google Scholar 

  43. H. Gomes, Thesis, Universidad Esadual de Compinas SP (Brazil), Faculdade de Engenbaric de Alimentis, Dept. de Technologia de Tecnologia de Alimentos. (2002)

  44. D.U. Ahn, K.C. Nam, Effects of ascorbic acid and antioxidants on color, lipid oxidation and volatiles of irradiated ground beef. Agric. Food Res. 2, 9–22 (2004)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Mohammad Hasan Abdeldaiem.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Abdeldaiem, M.H., Ali, H.G.M. & Foda, M.I. Improving the quality of minced beef by using mulberry leaves extract. Food Measure 11, 1681–1689 (2017). https://doi.org/10.1007/s11694-017-9548-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11694-017-9548-8

Keyword

Navigation