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In Vitro and In Vivo Antibacterial Activity of Patchouli Alcohol from Pogostemon cablin

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Abstract

Objective

To investigate the antibacterial activity of patchouli alcohol (PA) against 127 bacteria strains, including the common bacteria and drug-resistant bacteria strains both in the in vitro and in vivo tests.

Methods

For the in vitro trial, the antibacterial property of PA against 107 Gram-positive and 20 Gram-negative bacteria strains was screened by agar double dilution method. For the in vivo trial, specific pathogen free Kunming strain of both male and female white mice, were used to test the protective ability of PA after being injected with the median lethal dose of the tested strains.

Results

PA possessed antibacterial activity against all the tested 127 strains. In the in vitro test, PA could inhibit both Gram-negative bacteria (25–768μg/mL) and Gram-positive bacteria (1.5–200μg/mL). Particularly, PA was active against some drug-resistant bacteria like methicillin-resistant Staphylococcus aureus (MRSA). PA also exhibited in vivo anti-MRSA activity in mice via intraperitoneal injection. PA could protect mice entirely infected with MRSA at 100 and 200 mg/kg, while 80% mice injected with MRSA could be protected at a low dose of 50μg/mL.

Conclusion

PA might be a potential antibacterial drug from natural sources and might be worthy to explore its mechanism and application in further study.

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Authors and Affiliations

  1. School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China

    Feng Wan, Liang Xiong & Cheng Peng

  2. State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Sichuan Province and Ministry of Science and Technology, Chengdu, 610075, China

    Feng Wan, Liang Xiong & Cheng Peng

  3. School of Chinese Medicine, the University of Hong Kong, Hong Kong SAR, China

    Fu Peng & Jian-ping Chen

  4. School of Medical Laboratory Science, Chengdu Medical College, Chengdu, 610500, China

    Min Dai

Authors
  1. Feng Wan
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  2. Fu Peng
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  3. Liang Xiong
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  4. Jian-ping Chen
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  5. Cheng Peng
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  6. Min Dai
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Corresponding author

Correspondence to Cheng Peng.

Additional information

Supported by the National Key Technology Research and Development Program of China (No. 2009BAI84B00)

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Wan, F., Peng, F., Xiong, L. et al. In Vitro and In Vivo Antibacterial Activity of Patchouli Alcohol from Pogostemon cablin. Chin. J. Integr. Med. 27, 125–130 (2021). https://doi.org/10.1007/s11655-016-2452-y

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  • DOI: https://doi.org/10.1007/s11655-016-2452-y

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