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Anti-inflammatory Effects of Sanguisorbae Radix on Contact Dermatitis Induced by Dinitrofluorobenzene in Mice

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Abstract

Objective

To investigate the anti-inflflammatory effects of Sanguisorbae Radix on contact dermatitis (CD).

Methods

Mice were sensitized by painting 30 µL of 1-fluoro-2,4-dinitrofluorobenzene (DNFB) onto each ear for 3 days. Four days later, mice were challenged by painting with 50 µL of DNFB onto the shaved dorsum every 2 days. Sanguisorbae Radix methanol extract (MESR) was applied onto the shaved dorsum every 2 days. The effects of MESR on skin thickness, skin weights, histopathological changes, skin lesions and cytokine production in DNFB-induced CD mice were investigated, as well as its effects on body weights and spleen/body weight ratio.

Results

Topical application of MESR effectively inhibited enlargement of skin thickness and weight (P<0.05). MESR treatment also inhibited hyperplasia, spongiosis and immune cell infiltration induced by DNFB in inflamed tissues and improved lesions on dorsum skin in CD mice. Moreover, treatment with MESR suppressed the increase in the levels of tumor necrosis factor α (TNF-α,P<0.01) and interferon γ (IFN-γ,P<0.05), respectively. Finally, MESR had no effect on body weight gain or spleen/body weight ratio.

Conclusion

These data suggest that MESR acts as an anti-inflflammatory agent that decreases the production of TNF-α and IFN-γ, resulting in reductions of skin lesions and histopathological changes in inflamed skin tissues.

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Authors and Affiliations

  1. School of Korean Medicine, Pusan National University, Pusan, 626-870, Republic of Korea

    Suzy Jo, Junghyun Ryu, Hanna Kim, Miyoung Kim, Hyungwoo Kim & Su-In Cho

  2. School of Dentistry, Pusan National University, Pusan, 626-870, Republic of Korea

    Mi Heon Ryu

Authors
  1. Suzy Jo
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  2. Junghyun Ryu
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  3. Hanna Kim
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  4. Miyoung Kim
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  5. Mi Heon Ryu
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  6. Hyungwoo Kim
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  7. Su-In Cho
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Contributions

Conception and study design: Kim H, Cho SI; funding obtaining: Kim H; data acquisition: Jo S, Ryu J, Kim H, Kim M; data analysis and interpretation: Jo S, Ryu J, Kim H, Cho SI; statistical expertise: Ryu MH, Kim H, Cho SI; administrative technical/logistic support, data collection and assembly: Jo S, Ryu J, Kim H; critical revision of the article for important intellectual content: Ryu MH, Kim H; article drafting and final approval: all authors.

Corresponding author

Correspondence to Hyungwoo Kim.

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Conflict of Interest

The authors declare that there is no conflict of interest regarding the publication of this article.

Supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2015R1A2A2A04005619)

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Jo, S., Ryu, J., Kim, H. et al. Anti-inflammatory Effects of Sanguisorbae Radix on Contact Dermatitis Induced by Dinitrofluorobenzene in Mice. Chin. J. Integr. Med. 26, 688–693 (2020). https://doi.org/10.1007/s11655-015-2148-8

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  • DOI: https://doi.org/10.1007/s11655-015-2148-8

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