Abstract
Objective
To investigate the protective effect of Zengye Decoction (增液汤, ZYD) on the submandibular glands (SMGs) in nonobese diabetic (NOD) mice.
Methods
Twenty-seven female NOD mice were randomly equally divided into 3 groups: the model group, the hydroxychloroquine (HCQ) group, and the ZYD group. Nine C57/B6 mice served as the normal group. After 1-week acclimation, the HCQ and ZYD groups were intragastrically administered with HCQ and ZYD, respectively, and the normal and model groups were administered with normal saline. Changes in the salivary flow rate were observed. Mice from all 4 groups were sacrificed at the age of 20 weeks. The serum and SMGs were collected. Serum cytokines gamma-interferon (IFN-γ), interleukin-10 (IL-10) were detected by enzyme-linked immunosorbent assay. Histological changes in the submandibular glands were examined by hematoxylin and eosin staining. The mRNA expression of IFN-γ, IL-10 and vasoactive intestinal peptide (VIP) in the submandibular glands were measured by real-time polymerase chain reaction.
Results
Compared with the model group, the salivary flow of the ZYD group significantly increased (P<0.05), the extent of the histological changes was ameliorated (P<0.05), and the Th1/Th2 cytokine imbalance was remedied (P<0.05). In the ZYD-treated mice, the VIP mRNA was up-regulated (P<0.05).
Conclusions
ZYD is beneficial in protecting structure and function of SMGs in NOD mice. The mechanism may be associated with the correction of the Th1/Th2 cytokine imbalance, and with the prevention of a progressive decline of the VIP level.
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Supported by the National Natural Science Foundation of China (No. 81102525/H2701 and 81473607/H2708), Funds for Key Project Medical Science and Technology Development Foundation from Nanjing Department of Health (No. YKK11043), Funds for Opening Project from Nanjing university of Traditional Chinese Medicine (No. ZYNK003), and Funds for Graduate Student Innovation Project of Jiangsu Province, China (No. CXZZ13_0608)
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Li, Cy., Wu, Sl., Sun, Lx. et al. Protective Effect of Zengye Decoction (增液汤) on Submandibular Glands in Nonobese Diabetic Mice. Chin. J. Integr. Med. 25, 45–50 (2019). https://doi.org/10.1007/s11655-014-1981-5
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DOI: https://doi.org/10.1007/s11655-014-1981-5