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Antioxidant effect of salvianolic acid B on hippocampal CA1 neurons in mice with cerebral ischemia and reperfusion injury

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Abstract

Objetive

To investigate the neuroprotective effects and underlying mechanisms of salvianolic acid B (Sal B) extracted from Salvia miltiorrhiza on hippocampal CA1 neurons in mice with cerebral ischemia reperfusion injury.

Methods

Forty male National Institute of Health (NIH) mice were randomly divided into 4 groups with 10 animals each, including the sham group, the model group, the SalB group (SalB 22.5 mg/kg) and the nimodipine (Nim) group (Nim 1 mg/kg). A mouse model of cerebral ischemia and reperfusion injury was established by bilateral carotid artery occlusion for 30 min followed by 24-h reperfusion. The malondialdehyde (MDA) content, the nitric oxide synthase (NOS) activity, the superoxide dismutase (SOD) activity and total antioxidant capability (T-AOC) of the pallium were determined by biochemistry methods. The morphologic changes and Bcl-2 and Bax protein expression in hippocampal CA1 neurons were observed by using hematoxylineosin staining and immunohistochemistry staining, respectively.

Results

In the SalB group, the MDA content and the NOS activity of the pallium in cerebral ischemia-reperfusion mice significantly decreased and the SOD activity and the T-AOC significantly increased, as compared with the model group (P<0.05 or P<0.01). The SalB treatment also rescued neuronal loss (P<0.01) in the hippocampal CA1 region, strongly promoted Bcl-2 protein expression (P<0.01) and inhibited Bax protein expression (P<0.05).

Conclusions

SalB increases the level of antioxidant substances and decreases free radicals production. Moreover, it also improves Bcl-2 expression and reduces Bax expression. SalB may exert the neuroprotective effect through mitochondria-dependent pathway on hippocampal CA1 neurons in mice with cerebral ischemia and reperfusion injury and suggested that SalB represents a promising candidate for the prevention and treatment of ischemic cerebrovascular disease.

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Authors and Affiliations

  1. Department of Pathology, Beijing University of Chinese Medicine, Beijing, 100029, China

    Yu-feng Jiang  (蒋玉凤), Jia-pei Gong  (龚家培), Xi-mei Wang  (王玺玫), Ying Zhang  (张颖) & Mei-juan Yang  (杨美娟)

  2. Department of Pharmacology, College of Pharmacy, Hebei University, Baoding, Hebei Province, 071002, China

    Zhi-qin Liu  (刘智勤)

  3. Beijing Institute of Heart, Lung and Vessel Diseases, Beijing Anzhen Hospital, Capital University of Medical Sciences, Beijing, 100029, China

    Wei Cui  (崔巍)

  4. Department of Province Neurology, Beijing Huimin Hospital, Beijing, 100054, China

    Wen-tong Zhang  (张文通)

Authors
  1. Yu-feng Jiang  (蒋玉凤)
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  2. Zhi-qin Liu  (刘智勤)
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  3. Wei Cui  (崔巍)
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  4. Wen-tong Zhang  (张文通)
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  5. Jia-pei Gong  (龚家培)
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  6. Xi-mei Wang  (王玺玫)
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  7. Ying Zhang  (张颖)
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  8. Mei-juan Yang  (杨美娟)
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Corresponding author

Correspondence to Yu-feng Jiang  (蒋玉凤).

Additional information

Supported by the National Natural Science Foundation of China (No. 30472281)

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Jiang, Yf., Liu, Zq., Cui, W. et al. Antioxidant effect of salvianolic acid B on hippocampal CA1 neurons in mice with cerebral ischemia and reperfusion injury. Chin. J. Integr. Med. 21, 516–522 (2015). https://doi.org/10.1007/s11655-014-1791-1

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  • DOI: https://doi.org/10.1007/s11655-014-1791-1

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