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In vitro propagation of the endangered species Berberis aristata DC. via leaf-derived callus

  • Micropropagation
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Abstract

Berberis aristata, an endangered plant, is an important component in different medicinal formulations. The low seed germination rate coupled with fructification at high altitudes during the rainy season makes the task of seed collection difficult. In order to increase the yield of relevant plant material for medicinal and conservation purposes, an efficient in vitro regeneration protocol is important. Regeneration of endangered B. aristata from leaf-derived callus may offer an efficient alternative to natural propagation through seeds. When compared with nodal segments taken from in vitro-germinated seedlings and inoculated onto the same medium, leaf-derived callus was found to be better for shoot induction and multiplication. In optimization experiments, the rate of shoot multiplication from leaf-derived callus was increased from 17.6 ± 0.6 to 26.5 ± 0.2 shoots per callus by supplementing Woody Plant (WP) medium with 0.5 μM thidiazuron (TDZ) in place of 8.88 μM benzyladenine (BA) + 2.68 μM naphthalene acetic acid (NAA). Although shoot length was decreased when using TDZ, this could be alleviated by gibberellic acid (GA3) treatment. Rooting was achieved on half-strength WP medium supplemented with indole butyric acid (IBA) in either a one- or two-step rooting method. The two-step rooting procedure was found to be superior; under the best overall conditions, 55.6 ± 2.1% of plantlets formed roots within 25.7 ± 0.3 d. Well-rooted plantlets were successfully established in earthen pots under greenhouse conditions, with a 61.1% survival rate. This procedure is suitable for large-scale production of plants via in vitro propagation for this endangered plant species.

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References

  • Ahmed F, Rafii MY, Ismail MR, Juraimi AS, Rahim HA, Asfaliza R, Latif MA (2013) Waterlogging tolerance of crops: breeding, mechanism of tolerance, molecular approaches, and future prospects. BioMed Res Int 2013:963525. doi:10.1155/2013/963525

    PubMed Central  CAS  PubMed  Google Scholar 

  • Ali M, Malik AR, Sharma KR (2008) Vegetative propagation of Berberis aristata DC. An endangered Himalayan shrub. J Med Plant Res 2:374–377

    Google Scholar 

  • Anonymous (1988) The wealth of India: Berberis Linn. (Berberidaceae). Ambastha SP Publication and Information Directorate, CSIR, New Delhi, pp 114–118

  • Bhatt ID, Rawal RS, Dhar U (2000) Improvement in seed germination of Myrica esculenta Buch.-Ham. ex D. Don—a high value tree species of Kumaun Himalaya, India. Seed Sci Technol 28:597–605

    Google Scholar 

  • Das S, Mazumder PM, Das S, Das MK (2011) Phyto-pharmacology of Berberis aristata DC: a review. J Drug Deliv Ther 1:46–50

    Google Scholar 

  • Dhar S, Wakhlu AK, Sharma YP (2012) In vitro plant regeneration system for Berberis lycium using cotyledonary node explant. J Trop Med Plants 13:51–55

    Google Scholar 

  • Haque AU, Samad MA, Shapla TL (2009) In vitro callus induction and regeneration of potato. Bangladesh J Agric Res 34:449–456

    Article  Google Scholar 

  • Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult 33:105–119

    Article  CAS  Google Scholar 

  • Kala CP (2002) Indigenous knowledge of Bhotiya tribal community on wool dyeing and its present status in the Garhwal Himalaya, India. Curr Sci 83:814–817

    Google Scholar 

  • Kende H (1989) Enzymes of ethylene biosynthesis. Plant Physiol 91:1–4

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kirtikar KR, Basu BD (1933) Indian medicinal plants, vol I. Lalit Mohan Basu and Co, Allahabad, pp 100–108

    Google Scholar 

  • Lloyd G, McCown B (1980) Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by shoot-tip culture. Comb Proc Int Plant Prop Soc 30:421–427

    Google Scholar 

  • Mehta J, Ansari R, Syedy M, Khan S, Sharma S, Gupta N, Rathore R, Vaishnav K (2012) An effective method for high frequency multiple shoots regeneration and callus induction of Bacopa monnieri (L.) Pennel: an important medicinal plant. Asian J Plant Sci Res 2:620–626

    CAS  Google Scholar 

  • Mackay WA, Molinar F Jr, Wall MM, Cardenas M (1996) Micropropagation of agarita (Berberis trifoliata Moric.). HortSci 31:1030–1032

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Murthy BNS, Saxena PK (1998) Somatic embryogenesis and plant regeneration of neem (Azadirachta indica A. Juss.). Plant Cell Rep 17:469–475

    Article  CAS  Google Scholar 

  • Pandey A, Brijwal L, Tamta S (2013) In vitro propagation and phytochemical assessment of Berberis chitria: an important medicinal shrub of Kumaun Himalaya, India. J Med Plant Res 7:930–937

    CAS  Google Scholar 

  • Pandey A, Tamta S (2014) In vitro propagation of the important tasar oak (Quercus serrata Thunb.) by casein hydrolysate promoted high frequency shoot proliferation. J Sustain For 33:590–603

    Article  Google Scholar 

  • Purohit VK, Palni LMS, Nandi SK (2009) Effect of pre-germination treatments on seed physiology and germination of central Himalayan oaks. Physiol Mol Biol Plants 15:319–326

    Article  PubMed Central  PubMed  Google Scholar 

  • Semwal BC, Gupta J, Singh S, Kumar Y, Giri M (2009) Antihyperglycemic activity of root of Berberis aristata DC. in alloxan induced diabetic rat. Intl J Green Pharm 3:259–262

    Article  Google Scholar 

  • Sharma C, Aneja KR, Kesera R (2011) Screening of Berberis aristata DC. for antimicrobial potential against the pathogens causing ear infection. Int J Pharmacol 7:536–541

    Article  Google Scholar 

  • Singh A, Duggal S, Kaur N, Singh J (2010) Berberin: alkaloid with wide spectrum of pharmacological activities. J Nat Prod 3:64–75

    CAS  Google Scholar 

  • Tamta S, Palni LMS, Purohit VK, Nandi SK (2008) In vitro propagation of brown oak (Quercus semecarpifolia Sm.) from seedling explants. In Vitro Cell Dev Biol Plant 44:136–141

    Article  CAS  Google Scholar 

  • Vengadesan G, Pijut PM (2009) In vitro propagation of northern red oak (Quercus rubra L.). In Vitro Cell Dev Biol Plant 45:474–482

    Article  CAS  Google Scholar 

  • Vogth AR (1970) Effect of gibberellic acid on germination and initial seedling growth of northern red oak. For Sci 16:453–459

    Google Scholar 

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Acknowledgments

The authors would like to express their gratitude to Uttarakhand State Biotechnology Department for financial support and extend special thanks to Professor (Dr.) Y.P.S. Pangtey (Taxonomist in the Department of Botany, D.S.B. Campus, Kumaun University, Nainital, Uttarakhand, India), for identifying the plant sample of B. aristata, which is endangered and difficult to find in its natural habitat.

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Authors and Affiliations

  1. Plant Tissue Culture and Molecular Biology Laboratory, Department of Biotechnology, Kumaun University, Bhimtal Campus, Nainital, 263136, Uttarakhand, India

    Latika Brijwal

  2. G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal Almora, Uttarakhand, India

    Aseesh Pandey

  3. Plant Tissue Culture Laboratory, Department of Botany, Kumaun University, D.S.B. Campus, Nainital, 263002, Uttarakhand, India

    Sushma Tamta

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  1. Latika Brijwal
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  2. Aseesh Pandey
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  3. Sushma Tamta
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Correspondence to Sushma Tamta.

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Editor: Charles L. Armstrong

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Brijwal, L., Pandey, A. & Tamta, S. In vitro propagation of the endangered species Berberis aristata DC. via leaf-derived callus. In Vitro Cell.Dev.Biol.-Plant 51, 637–647 (2015). https://doi.org/10.1007/s11627-015-9716-7

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  • DOI: https://doi.org/10.1007/s11627-015-9716-7

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