Abstract
The squash bug, Anasa tristis, is a pest of cucurbits that exerts direct damage on crops and is a vector of plant pathogens. We established cell lines from this insect to serve as tools for basic biology, including virology and immunology, as well as applied studies, such as insecticide development programs. We initiated 15 cell cultures, using nine media or combinations of media. The media yielding the best results were a modification of Kimura’s medium and a combination of two commercially available cell culture media (EX-CELL 420 and L15). We designated the two cell lines as BCIRL-AtE-CLG11 and BCIRL-AtE-CLG15. From the AtE-CLG15 line, we isolated two sub-lines, A and B. Of these, the most consistently replicating line was AtE-CLG15A. We determined the doubling time of this line (190 h) and its mean cell diameter (14.5 ± 0.7 μm). We characterized the AtE-CLG15A line using DAF-PCR. The BCIRL-AtE-CLG15A cell line is now available for researchers world-wide.
References
Chiu R-J, Black LM (1967) Monolayer cultures of insect cell lines and their inoculation with a plant virus. Nature 215:1076–1078
Doughty HB, Wilson JM, Schultz PB, Kuhar TP (2016) Squash bug (Hemiptera: Coreidae): biology and management in cucurbitaceous crops. J Integr Pest Manag 7:1–8
Goodman CL, El Sayed GN, McIntosh AH, Grasela JJ, Stiles B (2001) Establishment and characterization of insect cell lines from 10 lepidopteran species. In Vitro Cell Dev Biol – Animal 37:367–373
Goodman CL, Stanley D, Ringbauer JA Jr, Beeman R, Silver K, Park Y (2012) A cell line derived from the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae). In Vitro Dev Biol – Animal 48:426–433
Hink WF, Ellis BJ (1971) 3. Establishment and characterization of two new cell lines (CP-1268 and CP-169) from the codling moth, Carpocapsa pomonella (with a review of culture of cells and tissues from Lepidoptera). Curr Top Microbiol Immunol 55:19–28
Hunter WB, Polston JE (2001) Development of a continuous whitefly cell line [Homoptera: Aleyrodidae: Bemisia tabaci (Gennadius)] for the study of Begomovirus. J Invertbr Pathol 77:33–36
Kamita SG, Do ZN, Samra AI, Hagler JR, Hammock BD (2005) Characterization of cell lines developed from the glassy-winged sharpshooter, Homalodisca coagulata (Hemiptera: Cicadellidae). In Vitro Cell Dev Biol – Animal 41:149–153
Kimura I (1984) Establishment of new cell lines from leafhopper vector and inoculation of its cell monolayers with rice dwarf virus. Proc Japan Acad 60B:198–201 (in English)
McIntosh A, Grasela JJ, Matteri RL (1996) Identification of insect cell lines by DNA amplification fingerprinting (DAF). Insect Molec Biol 5:187–195
Pant U, Dhanda V, Aragade SP, Banerjee K (1988) Establishment of three cell line form the embryonic tissue of Cimex hemipterus (F.) (Hemiptera: Cimicidae). In Vitro Cell Dev Biol 24:1201–1203
Pudney M, Lanar D (1977) Establishment and characterization of a cell line (BTC-32) from the triatomine bug, Triatoma infestans (Klug) (Hemiptera: Reduviidae). Ann Trop Med Parasitol 71(1):109–118
Richardson J, Jensen DD (1971) Tissue culture of monolayer cell lines of Colladonus montanus (Homoptera: Cicadellidae) a vector of the causal agent of western X-disease of peach. Ann Entomol Soc Amer 64:722–729
Smagghe G, Goodman CL, Stanley D (2009) Insect cell culture and applications to research and pest management. In Vitro Cell Dev Biol—Animal 45:93–105
Wayadande AC, Fletcher J (1998) Development and use of an established cell line of the leafhopper Circulifer tenellus to characterize Spiroplasma citri-vector interactions. J Invertbr Pathol 72:126–131
Acknowledgments
Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. All programs and services of the U.S. Department of Agriculture are offered on a nondiscriminatory basis without regard to race, color, national origin, religion, sex, age, marital status, or handicap.
Author information
Authors and Affiliations
Corresponding author
Additional information
Editor: Tetsuji Okamoto
Rights and permissions
About this article
Cite this article
Goodman, C.L., Ringbauer, J.A., Li, YF. et al. Cell lines derived from the squash bug, Anasa tristis (Coreidae: Hemiptera). In Vitro Cell.Dev.Biol.-Animal 53, 417–420 (2017). https://doi.org/10.1007/s11626-017-0134-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11626-017-0134-5