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Cytotoxicity and Antioxidative Effects of Herbal and Fruit Extracts In Vitro

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Abstract

Many studies have been carried out on bioactivities of individual herbs/fruits using in cosmetics or as a diet products, however, no collective study on their comparative antioxidant activities against oxidative damage or on cytotoxicity effect has been reported. The aim of this work was study the cytotoxicity and antioxidative activity of eight extracts with hypothetical antioxidative influence in vitro. To further elucidate of a possible role of herbals/fruits extracts on cell protection was used on the healthy and UV-A damaged mouse fibroblast cells. The cell viability was detect using MTT assay. Kinetic production of reactive oxygen species, identification of cell death, cell cycle and gene expression C-FOS were measured. Intracellular and mitochondrial transmembrane potential was evaluate with JC-1 fluorescence probe. Comet assay was employed to detect the UV-A induced DNA damage. The results indicated that using the extracts decreased ROS production. It can lead to greatly enhance and promote the viability of cells. As the most effective antioxidant in quenching of the ROS, in cell viability and DNA presentation was determined Prunella Vulgaris. However, one of them (Wheat Germ Oil) caused increased production of ROS and low cell viability.

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Abbreviations

(ROS):

reactive oxygen species

(MMP):

mitochondrial membrane potential

(UV):

ultraviolet

(UV-A):

ultraviolet A

(DMEM):

Dulbecco’s modified Eagle’s medium

(FBS):

fetal bovine sefum

(PI):

propidium iodide

(PS):

phosphatidylserine

(C):

Catechin

(EC):

Epicatechin

(GC):

Gallocatechin

(EGC):

Epigallocatechin

(ECG):

Epicatechin-3-gallate

(EGCG):

Epigallocatechin-3-gallate

IC50:

50 % inhibition concentration

(DPPH):

2,2-diphenyl-1-picrylhydrazyl

(LDL):

Low-density lipoprotein

(NMDA):

N-methyl D-aspartate

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Acknowledgments

This work was supported by the Grant Project LF_2014_003, IGA MZCR NT 14060-3/2013. Thanks to Jana Zapletalova for statistic analysis.

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Authors and Affiliations

  1. Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Hnevotinska 3, 775 15, Olomouc, Czech Republic

    Katerina Tomankova, Hana Kolarova, Klara Pizova, Svatopluk Binder, Lukas Malina, Jana Horakova & Jakub Malohlava

  2. Institute of Translation Medicine, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Hnevotinska 3, 775 15, Olomouc, Czech Republic

    Petr Konecny

  3. Department of Immunology, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Hnevotinska 3, 775 15, Olomouc, Czech Republic

    Eva Kriegova

  4. National Institute of Public Health, Srobarova 48, 100 42, Prague, Czech Republic

    Kristina Kejlova & Dagmar Jirova

Authors
  1. Katerina Tomankova
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  2. Hana Kolarova
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Correspondence to Katerina Tomankova.

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Tomankova, K., Kolarova, H., Pizova, K. et al. Cytotoxicity and Antioxidative Effects of Herbal and Fruit Extracts In Vitro. Food Biophysics 9, 267–276 (2014). https://doi.org/10.1007/s11483-014-9349-0

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  • DOI: https://doi.org/10.1007/s11483-014-9349-0

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