Abstract
By using packed soil-core incubation experiments, we have studied stimulating effects of addition of external carbon (C) (glucose, 6.4 g C m−2) on heterotrophic respiration and microbial biomass C of a mature broadleaf and Korean pine mixed forest (BKPF) and an adjacent white birch forest (WBF) soil under different wetting intensities (55% and 80% WFPS, water-filled pore space) and nitrogen (N) supply (NH4Cl and KNO3, 4.5 g N m−2) conditions. The results showed that for the control, the cumulative carbon dioxide (CO2) flux from WBF soil during the 15-day incubation ranged from 5.44 to 5.82 g CO2-C m−2, which was significantly larger than that from BKPF soil (2.86 to 3.36 g CO2-C m−2). With increasing wetting intensity, the cumulative CO2 flux from the control was decreased for the WBF soil, whereas an increase in the CO2 flux was observed in the BKPF soil (P < 0.05). The addition of NH4Cl or KNO3 alone significantly reduced the cumulative CO2 fluxes by 9.2%–21.6 % from the two soils, especially from WBF soil at low wetting intensity. The addition of glucose alone significantly increased soil heterotrophic respiration, microbial biomass C (MBC), and microbial metabolic quotient. The glucose-induced cumulative CO2 fluxes and soil MBC during the incubation ranged from 8.7 to 11.7 g CO2-C m−2 and from 7.4 to 23.9 g C m−2, which are larger than the dose of added C. Hence, the addition of external carbon can increase the decomposition of soil native organic C. The glucose-induced average and maximum rates of CO2 fluxes during the incubation were significantly influenced by wetting intensity (WI) and vegetation type (VT), and by WI×VT, NH4Cl×VT and WI×VT×NH4Cl (P<0.05). The addition of NH4Cl, instead of KNO3, significantly decreased the glucose-induced MBC of WBF soil (P<0.05), whereas adding NH4Cl and KNO3 both significantly increased the glucose-induced MBC of BKPF soil at high moisture (P<0.05). According to the differences in soil labile C pools, MBC and CO2 fluxes in the presence and absence of glucose, it can be concluded that the stimulating effects of glucose on soil heterotrophic respiration and MBC under temperate forests were dependent on vegetation type, soil moisture, and amount and type of the N added.
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References
Asmar F, Eiland F, Nielsen N E. 1994. Effect of extracellular-enzyme activities on solubilization rate of soil organic nitrogen. Biol Fert Soils, 17: 32–38
Banning N C, Grant C D, Jones D L, et al. 2008. Recovery of soil organic matter, organic matter turnover and nitrogen cycling in a post-mining forest rehabilitation chronosequence. Soil Biol Biochem, 40: 2021–2031
Bell J M, Smith J L, Bailey V L, et al. 2003. Priming effect and C storage in semi-arid no-till spring crop rotations. Biol Fert Soils, 37: 237–244
Blagodatskaya E V, Blagodatsky S A, Anderson T H, et al. 2007. Priming effects in chernozem induced by glucose and n in relation to microbial growth strategies. Appl Soil Ecol, 37: 95–105
Blagodatskaya E, Kuzyakov Y. 2008. Mechanisms of real and apparent priming effects and their dependence on soil microbial biomass and community structure: Critical review. Biol Fert Soils, 45: 115–131
Bowden R D, Davidson E, Savage K, et al. 2004. Chronic nitrogen additions reduce total soil respiration and microbial respiration in temperate forest soils at the harvard forest. For Ecol Manage, 196: 43–56
Chen C M, Xie Z B, Zhu J G. 2006. Advances in research on priming effect of soil organic carbon (in Chinese). Soils, 38: 359–365
Compton J E, Watrud L S, Porteous L A, et al. 2004. Response of soil microbial biomass and community composition to chronic nitrogen additions at harvard forest. For Ecol Manage, 196: 143–158
Conde E, Cardenas M, Ponce-Mendoza A, et al. 2005. The impacts of inorganic nitrogen application on mineralization of 14C-labelled maize and glucose, and on priming effect in saline alkaline soil. Soil Biol Biochem, 37: 681–691
Dalenberg J W, Jager G. 1989. Priming effect of some organic additions to C14-labeled soil. Soil Biol Biochem, 21: 443–448
Dijkstra F A, Cheng W X. 2007. Moisture modulates rhizosphere effects on C decomposition in two different soil types. Soil Biol Biochem, 39: 2264–2274
Dilly O, Zyakun A. 2008. Priming effect and respiratory quotient in a forest soil amended with glucose. Geomicrobiol J, 25: 425–431
Ding W X, Yu H Y, Cai Z C, et al. 2010. Responses of soil respiration to N fertilization in a loamy soil under maize cultivation. Geoderma, 155: 381–389
Dixon R K, Brown S, Houghton R A, et al. 1994. Carbon pools and flux of global forest ecosystems. Science, 263: 185–190
Falchini L, Naumova N, Kuikman P J, et al. 2003. CO2 evolution and denaturing gradient gel electrophoresis profiles of bacterial communities in soil following addition of low molecular weight substrates to simulate root exudation. Soil Biol Biochem, 35: 775–782
Fontaine S, Barot S, Barre P, et al. 2007. Stability of organic carbon in deep soil layers controlled by fresh carbon supply. Nature, 450: 277–210
Franzluebbers A J. 1999. Microbial activity in response to water-filled pore space of variably eroded southern piedmont soils. Appl Soil Ecol, 11: 91–101
Hamer U, Marschner B. 2005. Priming effects in different soil types induced by fructose, alanine, oxalic acid and catechol additions. Soil Biol Biochem, 37: 445–454
Huang W Z, Zhao X L, Zhu J G, et al. 2007. Priming effect of soil carbon pools (in Chinese). Chin J Soil Sci, 38: 149–154
Intergovernmental Panel on Climate Change (IPCC). 2013. Fifth Assessment Report (AR5), Working Group I: Thephysical Science Basis. Cambridge: Cambridge University Press. 475–475
Iqbal J, Hu R G, Feng M L, et al. 2010. Microbial biomass, and dissolved organic carbon and nitrogen strongly affect soil respiration in different land uses: A case study at Three Gorges Reservoir Area, South China. Agric Ecosyst Environ, 137: 294–307
Jenkinson D S, Fox R H, Rayner J H. 1985. Interactions between fertilizer nitrogen and soil-nitrogen—the so-called priming effect. J Soil Sci, 36: 425–444
Jenkinson D S. 1988. Determination of microbial biomass carbon and nitrogen in soil. In: Wilson J R, ed. Advances in Nitrogen Cycling in Agricultural Ecosystems. Wallingford: CAB International. 368–386
Jia F L, Li Z P, Liu M, et al. 2012. Effects of glucose and ammonium sulfate addition on paddy soil microbial biomass and functional diversity (in Chinese). Sci Agric Sin, 11: 2199–2208
Kuzyakov Y, Friedel J K, Stahr K. 2000. Review of mechanisms and quantification of priming effects. Soil Biol Biochem, 32: 1485–1498
Kuzyakov Y. 2010. Priming effects: Interactions between living and dead organic matter. Soil Biol Biochem, 42: 1363–1371
Li Y Q, Xu M, Zou X M. 2006. Heterotrophic soil respiration in relation to environmental factors and microbial biomass in two wet tropical forests. Plant Soil, 281: 193–201
Liski J, Nissinen A, Erhard M, et al. 2003. Climatic effects on litter decomposition from arctic tundra to tropical rainforest. Glob Change Biol, 9: 575–584
Liu H S, Li L H, Han X G, et al. 2006. Respiratory substrate availability plays a crucial role in the response of soil respiration to environmental factors. Appl Soil Ecol, 32: 284–292
Liu X Z, Wan S Q, Su B, et al. 2002. Response of soil CO2 efflux to water manipulation in a tallgrass prairie ecosystem. Plant Soil, 240: 213–223
Luo Y, Durenkamp M, De Nobili M, et al. 2011. Short term soil priming effects and the mineralisation of biochar following its incorporation to soils of different pH. Soil Biol Biochem, 43: 2304–2314
Lv D Q, Zhang S L, Yang X Y. 2007. Effect of supplying C and N on the mineralization and priming effect of organic matter in loessial soil (in Chinese). Plant Nutr Fert Sci, 13: 423–429
Ma Q X, Li W, Pan K W, et al. 2013. Effect of continuous glucose addition on soil N transformation of the pinus tabulaeformis and cercidiphyllum japonicum plantations (in Chinese). Chin J Appl Environ Biol, 19: 426–433
Paterson E, Sim A. 2013. Soil-specific response functions of organic matter mineralization to the availability of labile carbon. Glob Change Biol, 19: 1562–1571
Persson T, Karlsson P S, Seyferth U. 2000. Carbon mineralization in European forest soils. In: Schulze E D, ed. Ecological Studies-Carbon and Nitrogen Cycling in European Forest Ecosystems. Berlin: Springer. 257–275
Schneckenberger K, Demin D, Stahr K, et al. 2008. Microbial utilization and mineralization of (14C) glucose added in six orders of concentration to soil. Soil Biol Biochem, 40: 1981–1988
Smith V R. 2005. Moisture, carbon and inorganic nutrient controls of soil respiration at a sub-antarctic island. Soil Biol Biochem, 37: 81–91
Thirukkumaran C M, Parkinson D. 2000. Microbial respiration, biomass, metabolic quotient and litter decomposition in a lodgepole pine forest floor amended with nitrogen and phosphorous fertilizers. Soil Biol Biochem, 32: 59–66
Tian D L, Wang G J, Yan W D, et al. 2010. Soil respiration dynamics in cinnamomum camphora forest and a nearby liquidambar formosana forest in subtropical China. Chin Sci Bull, 55: 736–743
Wang H, Fan Z P, Deng D Z, et al. 2008. Effects of environm ental factors on soil organic carbon mineralization in a pinus sylvestris var. mongolica plantation (in Chinese). Chin J Ecol, 27: 1469–1475
Wang Q K, Xiao F M, He T X, et al. 2013. Responses of labile soil organic carbon and enzyme activity in mineral soils to forest conversion in the subtropics. Ann Forest Sci, 70: 579–587
Wang W, Guo J X. 2006. Partitioning root and microbial contributions to soil respiration in leymus chinensis populations. Chin Sci Bull, 38: 653–660
Wang X, Zhou G S, Jiang Y L, et al. 2007. Soil respiration in natural mixed (betula platyphylla and populus davidiana) secondary forest and primary broad-leaved korean pine forest (in Chinese). J Plant Ecol, 31: 348–354
Wang Y S, Wang Y H. 2003. Quick measurement of CH4, CO2 and N2O emissions from a short-plant ecosystem. Adv Atmos Sci, 20: 842–844
Wu H H, Xu X K, Li T S, et al. 2014. Effect of carbon and nitrogen addition on nitrous oxide flux from temperate forest soils under different wetting intensity conditions. Chin Sci Bull, 59: 1337–1347
Wu J S, Joergensen R G, Pommerening B, et al. 1990. Measurement of soil microbial biomass C by fumigation extraction—An automated procedure. Soil Biol Biochem, 22: 1167–1169
Wu Z T, Dijkstra P, Koch G W, et al. 2011. Responses of terrestrial ecosystems to temperature and precipitation change: A meta-analysis of experimental manipulation. Glob Change Biol, 17: 927–942
Xu X K, Han L, Wang Y S, et al. 2007. Influence of vegetation types and soil properties on microbial biomass carbon and metabolic quotients in temperate volcanic and tropical forest soils. Soil Sci Plant Nutr, 53: 430–440
Yamasaki A, Tateno R, Shibata H. 2011. Effects of carbon and nitrogen amendment on soil carbon and nitrogen mineralization in volcanic immature soil in southern kyushu. Japan: J For Res-JPN, 16: 414–423
Yoshitake S, Sasaki A, Uchida M, et al. 2007. Carbon and nitrogen limitation to microbial respiration and biomass in an acidic solfatara field. Eur J Soil Biol, 43: 1–13
Zhang T Y, Xu X K, Luo X B, et al. 2009. Effects of acetylene at low concentrations on nitrification, mineralization and microbial biomass nitrogen concentrations in forest soils. Chin Sci Bull, 54: 296–303
Zhao S C, Qiu S J, Cao C Y, et al. 2014. Responses of soil properties, microbial community and crop yields to various rates of nitrogen fertilization in a wheat-maize cropping system in north-central China. Agric Ecosyst Environ, 194: 29–37
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An erratum to this article is available at http://dx.doi.org/10.1007/s11430-017-9049-6.
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Wu, H., Xu, X., Duan, C. et al. Effect of vegetation type, wetting intensity, and nitrogen supply on external carbon stimulated heterotrophic respiration and microbial biomass carbon in forest soils. Sci. China Earth Sci. 58, 1446–1456 (2015). https://doi.org/10.1007/s11430-015-5058-x
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DOI: https://doi.org/10.1007/s11430-015-5058-x