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Fast determination of 24 steroid hormones in river water using magnetic dispersive solid phase extraction followed by liquid chromatography–tandem mass spectrometry

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Abstract

The easiness-to-handle of the magnetic dispersive solid phase extraction (Mag-dSPE) procedure was developed for preconcentration of 24 steroid hormones in river water. Ethylenediamine-functionalized magnetic carbon nanotubes (EDA@Mag-CNTs) were synthesised by a simple one-pot reaction and were used as sorbent in Mag-dSPE procedure. The properties of the EDA@Mag-CNTs were characterized by transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM). An ultra-fast liquid chromatography–tandem quadrupole mass spectrometry (UFLC-MS/MS) method for determination of 24 steroid hormones in river water at nanograms per liter had been developed with pretreatment of the samples by Mag-dSPE. The obtained results demonstrated the higher extraction capacity of EDA@Mag-CNT Mag-dSPE with recoveries between 82.1 and 113 %. The limits of quantification (LOQs) for the steroid hormone were between 0.020 and 1.00 ng/L. The developed method had been successfully applied to 60 river water samples, and it was confirmed that EDA@Mag-CNT Mag-dSPE was a highly effective extraction method for the steroid hormone analyses.

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Acknowledgments

We would like to thank the National Natural Science Foundation of China (No. 21377114), the Zhejiang Provincial Natural Science Foundation, China (No. LY12H26003, LY13B050003, LY14B040006), the Medical Health Foundation for Key Talents in Zhejiang Province, China (No.2013KYA187), Ningbo Natural Science Foundation of China (No. 2013A610242 and No. 2014A610283), Ningbo Medical Technology Foundation (No. 2013A24), the Science and Technology Foundation for Benefit people of Ningbo (No. 2015C50041), the Zhejiang Provincial Analytical Foundation of China (No. 2014C37037), and Zhejiang Provincial Program for the Cultivation of High-Level Innovative Health Talents for their financial support.

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Authors and Affiliations

  1. Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, Zhejiang, China

    Yong-Gang Zhao, Xiao-Hong Chen, Sheng-Dong Pan & Mi-Cong Jin

  2. Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, Zhejiang, China

    Yong-Gang Zhao, Xiao-Hong Chen, Sheng-Dong Pan & Mi-Cong Jin

  3. Quzhou Center for Disease Control and Prevention, Quzhou, 324000, Zhejiang, China

    Yun Zhang

  4. School of Marine Sciences, Ningbo University, Ningbo, 315211, Zhejiang, China

    Ping-Ping Zhan

Authors
  1. Yong-Gang Zhao
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  2. Yun Zhang
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  3. Ping-Ping Zhan
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  4. Xiao-Hong Chen
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  5. Sheng-Dong Pan
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  6. Mi-Cong Jin
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Correspondence to Mi-Cong Jin.

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Responsible editor: Ester Heath

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Fig. S1

VSM of nano Fe3O4 and EDA@Mag-CNTs (DOC 164 kb)

Fig. S2

The XRD patterns of EDA@Mag-CNTs (DOC 199 kb)

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Zhao, YG., Zhang, Y., Zhan, PP. et al. Fast determination of 24 steroid hormones in river water using magnetic dispersive solid phase extraction followed by liquid chromatography–tandem mass spectrometry. Environ Sci Pollut Res 23, 1529–1539 (2016). https://doi.org/10.1007/s11356-015-5399-1

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  • DOI: https://doi.org/10.1007/s11356-015-5399-1

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