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P2Y1 receptor activation by photolysis of caged ATP enhances neuronal network activity in the developing olfactory bulb

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Abstract

It has recently been shown that adenosine-5′-triphosphate (ATP) is released together with glutamate from sensory axons in the olfactory bulb, where it stimulates calcium signaling in glial cells, while responses in identified neurons to ATP have not been recorded in the olfactory bulb yet. We used photolysis of caged ATP to elicit a rapid rise in ATP and measured whole-cell current responses in mitral cells, the output neurons of the olfactory bulb, in acute mouse brain slices. Wide-field photolysis of caged ATP evoked an increase in synaptic inputs in mitral cells, indicating an ATP-dependent increase in network activity. The increase in synaptic activity was accompanied by calcium transients in the dendritic tuft of the mitral cell, as measured by confocal calcium imaging. The stimulating effect of ATP on the network activity could be mimicked by photo release of caged adenosine 5′-diphosphate, and was inhibited by the P2Y1 receptor antagonist MRS 2179. Local photolysis of caged ATP in the glomerulus innervated by the dendritic tuft of the recorded mitral cell elicited currents similar to those evoked by wide-field illumination. The results indicate that activation of P2Y1 receptors in the glomerulus can stimulate network activity in the olfactory bulb.

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Acknowledgments

Supported by the Deutsche Forschungsgemeinschaft (LO 779/6).

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Authors and Affiliations

  1. Division of Neurophysiology, Biocenter Grindel, University of Hamburg, Martin-Luther-King-Pl. 3, 20146, Hamburg, Germany

    Timo Fischer, Natalie Rotermund, Christian Lohr & Daniela Hirnet

Authors
  1. Timo Fischer
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  2. Natalie Rotermund
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  3. Christian Lohr
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  4. Daniela Hirnet
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Corresponding author

Correspondence to Daniela Hirnet.

Additional information

Timo Fischer and Natalie Rotermund contributed equally to the study.

Electronic supplementary material

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Supplementary figure 1

Analysis of the current response. The integral, i.e., the area enclosed by the current trace and the baseline (gray area) was calculated (JPEG 615 kb)

Supplementary figure 2

Olfactory bulb brain slice. a Mitral cell somata build the narrow band of the mitral cell layer (ML). b At high magnification, individual somata of mitral cells can be distinguished (asterisks; JPEG 996 kb)

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Fischer, T., Rotermund, N., Lohr, C. et al. P2Y1 receptor activation by photolysis of caged ATP enhances neuronal network activity in the developing olfactory bulb. Purinergic Signalling 8, 191–198 (2012). https://doi.org/10.1007/s11302-011-9286-z

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  • DOI: https://doi.org/10.1007/s11302-011-9286-z

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