Skip to main content
SpringerLink
Log in

Molecular cloning and expression analysis of the mevalonate diphosphate decarboxylase gene from the latexof Hevea brasiliensis

  • Original Article
  • Published:
Tree Genetics & Genomes Aims and scope Submit manuscript

Abstract

Mevalonate diphosphate decarboxylase (MVD) catalyzes the conversion of mevalonate diphosphate to isopentenyl diphosphate (IPP), a monomer of a large family of plant isoprenoids and cis-1,4-polyisoprene (natural rubber), which is present in most rubber-producing plants including Hevea brasiliensis (Para rubber tree). Here, we isolated the gene encoding MVD from the latex transcriptome of an elite rubber tree clone, Chinese Academy of Tropical Agriculture Sciences (CATAS) 7-33-97. The full-length cDNA of this gene, designated HbMVD, is 2032 bp long with a 1248-bp open reading frame (ORF) encoding a putative MVD of 415 amino acid residues with a theoretical molecular mass of 45.83 kDa and an isoelectric point (pI) of 6.52. Sequence alignment demonstrated that HbMVD cDNA shares 91 % identity with MVD genes from both Ricinus communis and Jatropha curcas. The genomic sequence of HbMVD is 11,749 bp long, comprising 10 exons and nine introns. The HbMVD promoter contains numerous cis-regulatory elements that are responsive to light, hormones, stress, and so on. Reverse-transcription quantitative PCR (qRT-PCR) revealed that HbMVD was most highly expressed in female flowers, followed by the bark, latex, male flowers, and leaves of untapped, mature rubber trees. The expression level of HbMVD in latex decreased progressively from the first, second, fourth, and third leaf stories of young shoots. Bark tapping (mechanical wounding), as well as exogenous ethephon and methyl jasmonate treatment, significantly upregulated HbMVD expression in the latex. This gene was upregulated in the bark and latex of tapping panel dryness (TPD)-affected trees but downregulated in latex from healthy, renewed bark. Further characterization of HbMVD would provide valuable insights into the involvement of HbMVD in regulating the biosynthesis of natural rubber and other IPP-derived isoprenoids in rubber tree.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11
Fig. 12
Fig. 13

Similar content being viewed by others

References

  • Abbassi SJ, Vishwakarma RK, Patel P, Kumari U, Khan BM (2015) Bacopa monniera recombinant mevalonate diphosphate decarboxylase: biochemical characterization. Int J Biol Macromol 79:661–668

    Article  CAS  PubMed  Google Scholar 

  • Berges T, Guyonnet D, Karst F (1997) Characterisation of a Leu-to-Pro mutation in a conserved sequence of mevalonate diphosphate decarboxylase in yeast. J Bacteriol 179:4664–4670

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bick JA, Lange BM (2003) Metabolic cross-talk between cytosolic and plastidial pathways of isoprenoid biosynthesis: unidirectional transport of intermediates across the chloroplast envelope membrane. Arch Biochem Biophys 415:146–154

    Article  CAS  PubMed  Google Scholar 

  • Bokma E, Spiering M, Chow KS, Mulder PP, Subroto T, Beintema JJ (2001) Determination of cDNA and genomic DNA sequences of hevamine, a chitinase from the rubber tree Hevea brasiliensis. Plant Physiol Biochem 39(5):367–376

    Article  CAS  Google Scholar 

  • Bouvier F, Rahier A, Camara B (2005) Biogenesis, molecular regulation and function of plant isoprenoids. Prog Lipid Res 44:357–429

    Article  CAS  PubMed  Google Scholar 

  • Chow KS, Wan KL, Mat-Isa MN, Bahari A, Tan SH, Harikrishna K, Yeang HY (2007) Insights into rubber biosynthesis from transcriptome analysis of Hevea brasiliensis latex. J Exp Bot 58:2429–2440

    Article  CAS  PubMed  Google Scholar 

  • Chow KS, Mat-Isa MN, Bahari A, Ghazali AK, Alias H, Zainuddin ZM, Hoh CC, Wan KL (2012) Metabolic routes affecting rubber biosynthesis in Hevea brasiliensis latex. J Exp Bot 63(5):1863–1871

    Article  CAS  PubMed  Google Scholar 

  • Duan C, Rio M, Leclercq J, Bonnot F, Oliver G, Montoro P (2010) Gene expression pattern in response to wounding, methyl jasmonate and ethylene in the bark of Hevea brasiliensis. Tree Physiol 30(10):1349–1359

    Article  CAS  PubMed  Google Scholar 

  • Eisenreich W, Rohdich F, Bacher A (2001) Deoxyxylulose phosphate pathway to terpenoids. Trends Plant Sci 6:78–84

    Article  CAS  PubMed  Google Scholar 

  • Hallahan DL, Keiper-Hrynko NM (2004) Genes involved in the biosynthesis of isopentenyl diphosphate in Hevea brasiliensis latex. EP, EP1434784

  • Hao BZ, Wu JL (2000) Laticifer differentiation in Hevea brasiliensis: induction by exogenous jasmonic acid and linolenic acid. Ann Bot 85(1):37–43

    Article  CAS  Google Scholar 

  • Hemmerlin A, HoeZer JF, Meyer O, Tritsch D, Kagan IA, Grosdemange-Billiard C, Rohmer M, Bach TJ (2003) Cross-talk between the cytosolic mevalonate and the plastidial methylerythritol phosphate pathways in tobacco bright yellow-2 cells. J Biol Chem 278:26666–26676

    Article  CAS  PubMed  Google Scholar 

  • Hsieh M, Chang C, Hsu S, Chen J (2008) Chloroplast localization of methylerythritol 4-phosphate pathway enzymes and regulation of mitochondrial genes in ispD and ispE albino mutants in Arabidopsis. Plant Mol Biol 66:663–673

    Article  CAS  PubMed  Google Scholar 

  • Kekwick RGO (1989) The formation of isoprenoids in Hevea latex. Physiology of rubber tree latex. CRC Press, Boca Raton, pp. 145–164

    Google Scholar 

  • Kirby J, Keasling JD (2009) Biosynthesis of plant isoprenoids: perspectives for microbial engineering. Annu Rev Plant Biol 60:335–355

    Article  CAS  PubMed  Google Scholar 

  • Ko JH, Chow KS, Han KH (2003) Transcriptome analysis reveals novel features of the molecular events occurring in the laticifers of Hevea brasiliensis (para rubber tree). Plant Mol Biol 53:479–492

    Article  CAS  PubMed  Google Scholar 

  • Koo AJK, Howe GA (2009) The wound hormone jasmonate. Phytochemistry 70(13):1571–1580

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Krishnakumar R, Jacob J (2003) Overproduction of endogenous ethylene inhibits rubber biosynthesis in Hevea brasiliensis. National symposium on Biochemical approaches for utilization and exploitation of commercially important plants. Indian Society of Agricultural Biochemists and Department of Agricultural Biochemistry Assam Agricultural University, Jorhat, Assam, Abstract p-59

  • Krishnakumar R, Jacob J (2006) Recent trends in research on tapping panel dryness syndrome. Preprints of papers: international natural rubber conference, India 2006, 13–14 November 2006, Ho Chi Minh, Vietnam, Rubber Research Institute of India, Kottayam, India, pp 657–666

  • Krishnakumar R, Chitra AN, Sreelatha S, Jacob J (2005) Ethephon stimulation triggers general adaptation syndrome (GAS) in Hevea brasiliensis. Preprints of papers: international natural rubber conference, India 2005, 6–8 November 2005, Cochin, India, Rubber Research Institute of India, Kottayam, India, pp 262–267

  • Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163

    Article  CAS  PubMed  Google Scholar 

  • Lange BM, Ghassemian M (2003) Genome organization in Arabidopsis thaliana: a survey for genes involved in isoprenoid and chlorophyll metabolism. Plant Mol Biol 51:925–948

    Article  CAS  PubMed  Google Scholar 

  • Lescot M, Dehais P, Thijs G, Marchal K, Moreau Y, van de Peer Y, Rouze P, Rombauts S (2002) PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Res 30:325–327

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lichtenthaler HK (1999) The 1-deoxy-D-xylulose-5-phosphate pathway of isoprenoid biosynthesis in plants. Annu Rev Plant Biol 50:47–65

    Article  CAS  Google Scholar 

  • Miziorko HM (2011) Enzymes of the mevalonate pathway of isoprenoid biosynthesis. Arch Biochem Biophys 505(2):131–143

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nandris D, Moreau R, de Fay E, Masson A, Pellegrin F, Chrestin H (2006) Etiological investigations on trunk phloem necrosis of the rubber tree: biotic or abiotic causal factors? Tapping panel dryness of rubber trees. Rubber Research Institute of India, Kottayam, pp. 85–92

    Google Scholar 

  • Nataraja KN, Prasad TG, Udayakumar M, Sathik MBM, Thomas M, Jacob J (2006) Hormone conflict: can impaired lateral meristems differentiation of latex vessels leading to tapping panel dryness in Hevea brasiliensis? Tapping panel dryness of rubber trees. Rubber Research Institute of India, Kottayam, pp. 125–129

    Google Scholar 

  • Nie Z, Kang G, Li Y, Dai L, Zeng R (2015) Whole-transcriptome survey of the putative ATP-binding cassette (ABC) transporter family genes in the latex-producing laticifers of Hevea brasiliensis. PLoS One 10(1):e0116857

    Article  Google Scholar 

  • Norton G, Pappusamy A, Yusof F, Pujade-Renaud V, Perkins M, Griffiths D, Jones H (2007) Characterisation of recombinant Hevea brasiliensis allene oxide synthase: effects of cycloxygenase inhibitors, lipoxygenase inhibitors and salicylates on enzyme activity. Plant Physiol Biochem 45(2):129–138

    Article  CAS  PubMed  Google Scholar 

  • Pal T, Malhotra N, Chanumolu SK, Chauhan RS (2015) Next-generation sequencing (NGS) transcriptomes reveal association of multiple genes and pathways contributing to secondary metabolites accumulation in tuberous roots of Aconitum heterophyllum Wall. Planta 242(1):239–258

    Article  CAS  PubMed  Google Scholar 

  • Pang Y, Shen GA, Bergès T, Cardier H, Wu W, Sun X, Tang K (2006) Molecular cloning, characterization and heterologous expression in Saccharomyces cerevisiae of a mevalonate diphosphate decarboxylase cDNA from Ginkgo biloba. Physiol Plantarum 127(1):19–27

    Article  CAS  Google Scholar 

  • Peng SQ, Xu J, Li HL, Tian WM (2009) Cloning and molecular characterization of HbCOI1 from Hevea brasiliensis. Biosci Biotech Bioch 73:665–670

    Article  CAS  Google Scholar 

  • Priyadarshan PM, Gonçalves PS, Omokhafe KO (2009) Breeding Hevea rubber. Breeding plantation tree crops: tropical species. Springer, New York, pp. 469–522

    Book  Google Scholar 

  • Rodriguez-Concepcion M (2006) Early steps in isoprenoid biosynthesis: multilevel regulation of the supply of common precursors in plant cells. Phytochem Rev 5:1–15

    Article  CAS  Google Scholar 

  • Rodriguez-Concepcion M (2010) Supply of precursors for carotenoid biosynthesis in plants. Arch Biochem Biophys 504:118–122

    Article  CAS  PubMed  Google Scholar 

  • Rodriguez-Concepcion M, Boronat A (2002) Elucidation of the methylerythritol phosphate pathway for isoprenoid biosynthesis in bacteria and plastids. A metabolic milestone achieved through genomics. Plant Physiol 130:1079–1089

    Article  CAS  PubMed  Google Scholar 

  • Sando T, Takaoka C, Mukai Y, Yamashita A, Hattori M, Ogasawara N, Fukusaki E, Kobayashi A (2008a) Cloning and characterization of mevalonate pathway genes in a natural rubber producing plant, Hevea brasiliensis. Biosci Biotech Bioch 72(8):2049–2060

    Article  CAS  Google Scholar 

  • Sando T, Takeno S, Watanabe N, Okumoto H, Kuzuyama T, Yamashita A, Hattori M, Ogasawara N, Fukusaki E, Kobayashi A (2008b) Cloning and characterization of the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway genes of a natural-rubber producing plant, Hevea brasiliensis. Biosci Biotech Bioch 72:2903–2917

    Article  CAS  Google Scholar 

  • Sando T, Hayashi T, Takeda T, Akiyama Y, Nakazawa Y, Fukusaki E, Kobayashi A (2009) Histochemical study of detailed laticifer structure and rubber biosynthesis-related protein localization in Hevea brasiliensis using spectral confocal laser scanning microscopy. Planta 230:215–225

    Article  CAS  PubMed  Google Scholar 

  • Simkin AJ, Guirimand G, Papon N, Courdavault V, Thabet I, Ginis O, Bouzid S, Giglioli-Guivarc’h N, Clastre M (2011) Peroxisomal localisation of the final steps of the mevalonic acid pathway in planta. Planta 234:903–914

    Article  CAS  PubMed  Google Scholar 

  • Steinbüchel A (2003) Production of rubber-like polymers by microorganisms. Curr Opin Microbiol 6:261–270

    Article  PubMed  Google Scholar 

  • Tan D, Sun X, Zhang J (2014) Age-dependent and jasmonic acid-induced laticifer-cell differentiation in anther callus cultures of rubber tree. Planta 240(2):337–344

    Article  CAS  PubMed  Google Scholar 

  • Tian WW, Huang WF, Zhao Y (2010) Cloning and characterization of HbJAZ1 from the laticifer cells in rubber tree (Hevea brasiliensis Muell. Arg.). Trees 24:771–779

    Article  CAS  Google Scholar 

  • Tian WM, Yang SG, Shi MJ, Zhang SX, Wu JL (2015) Mechanical wounding-induced laticifer differentiation in rubber tree: an indicative role of dehydration, hydrogen peroxide, and jasmonates. J Plant Physiol 182:95–103

    Article  CAS  PubMed  Google Scholar 

  • Wan CY, Wilkins TA (1994) A modified hot borate method significantly enhances the yield of high quality RNA from cotton (Gossypium hirsutum L.). Anal Biochem 223:7–12

    Article  CAS  PubMed  Google Scholar 

  • Wang KLC, Li H, Ecker JR (2002) Ethylene biosynthesis and signaling networks. Plant Cell 14(suppl 1):S131–S151

    CAS  PubMed  PubMed Central  Google Scholar 

  • Wu CT, Li Y, Dai LJ, Nie ZY, Kang GJ, Zeng RZ (2014) Cloning of sterol 14α-demethylase gene (HbCYP51) and effects of mechanical damage and exogenous phytohormones on its mRNA expression in Hevea brasiliensis. Chinese J Agr Biotech 22(12):1525–1535

    CAS  Google Scholar 

  • Zeng R, Duan C, Li X, Tian W, Nie Z (2009) Vacuolar-type inorganic pyrophosphatase located on the rubber particle in the latex is an essential enzyme in regulation of the rubber biosynthesis in Hevea brasiliensis. Plant Sci 176(5):602–607

    Article  CAS  Google Scholar 

  • Zhao Y, Zhou LM, Chen YY, Yang SG, Tian WM (2011) MYC genes with differential responses to tapping, mechanical wounding, ethrel and methyl jasmonate in laticifers of rubber tree (Hevea brasiliensis Muell. Arg.). J Plant Physiol 168:1649–1658

    Article  CAS  PubMed  Google Scholar 

  • Zulak KG, Bohlmann J (2010) Terpenoid biosynthesis and specialized vascular cells of conifer defense. J Integr Plant Biol 52:86–97

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31270713) and the Fundamental Research Funds for Rubber Research Institute, CATAS (No. 1630022012008).

Data archiving statement

The mRNA sequence data are available for download at http://www.ncbi.nlm.nih.gov/nuccore/KP677546. The promoter sequence data are available for download at http://www.ncbi.nlm.nih.gov/nuccore/KR781497. The DNA sequence data have been submitted to NCBI.

Author information

Authors and Affiliations

  1. Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), Danzhou, Hainan, 571737, People’s Republic of China

    Chuntai Wu, Yu Li, Zhiyi Nie, Longjun Dai, Guijuan Kang & Rizhong Zeng

  2. Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Danzhou, Hainan, People’s Republic of China

    Chuntai Wu, Yu Li, Zhiyi Nie, Longjun Dai, Guijuan Kang & Rizhong Zeng

Authors
  1. Chuntai Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yu Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhiyi Nie
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Longjun Dai
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Guijuan Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Rizhong Zeng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rizhong Zeng.

Additional information

Communicated by W. Ratnam

Electronic supplementary material

.

Table S1

(DOCX 26 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wu, C., Li, Y., Nie, Z. et al. Molecular cloning and expression analysis of the mevalonate diphosphate decarboxylase gene from the latexof Hevea brasiliensis . Tree Genetics & Genomes 13, 22 (2017). https://doi.org/10.1007/s11295-016-1091-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11295-016-1091-y

Keywords

Search

Navigation