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Identification of QTLs controlling seed dormancy in peach (Prunus persica)

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Abstract

Dormancy is a condition that delays or inhibits growth in seed, vegetative buds, and floral buds. In peach, seed germination occurs when seed accumulate sufficient stratification and growing degree hours to break dormancy and begin growing. Correlations have been reported between mean seed stratification requirements and mean bud chilling requirements among Prunus families, but an individual seed’s germination date and subsequent vegetative and floral bud break date are not correlated. Prior to this study, the genetic factors involved in regulating seed dormancy and their location on the peach genomic map were unknown. Segregating F2 seed were collected from a high × low chill F1 peach hybrid in 2005, 2006, and 2008. Germination date and growth habit was measured after the stratification requirement of the 2005 seed was fully met. The seed collected in 2006 and 2008 received varying amounts of stratification, which enabled data on stratification requirement, heat requirement, and growth habit to be collected. Genomic DNA was extracted from seedling leaf tissue and screened with SSR markers selected from the Prunus reference map at an average resolution of 20 cM. Seed dormancy quantitative trait loci (QTLs) were detected on G1, G4, G6/8, and G7. The QTLs detected on G6/8 and G7 were discovered in the same region as QTLs associated with floral bud chilling requirement and bloom time in peach.

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Abbreviations

AG:

Abnormal growth

CU:

Chilling unit

G:

Linkage group

GD:

Germination date

GDH:

Growing degree hour

HR:

Heat requirement

QTL:

Quantitative trait loci

SR:

Stratification requirement

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Authors and Affiliations

  1. University of Florida, Fifield Hall, Gainesville, FL, 32611, USA

    Kendra M. Blaker & José X. Chaparro

  2. Southeastern Fruit and Tree Nut Research Laboratory, USDA–Agricultural Research Service, 21 Dunbar Road, Byron, GA, 31008, USA

    Thomas G. Beckman

Authors
  1. Kendra M. Blaker
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  2. José X. Chaparro
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  3. Thomas G. Beckman
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Correspondence to Kendra M. Blaker.

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Blaker, K.M., Chaparro, J.X. & Beckman, T.G. Identification of QTLs controlling seed dormancy in peach (Prunus persica). Tree Genetics & Genomes 9, 659–668 (2013). https://doi.org/10.1007/s11295-012-0578-4

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  • DOI: https://doi.org/10.1007/s11295-012-0578-4

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