Abstract
The potential of Parthenium sp. as a feedstock for enzymatic saccharification was investigated by using chemical and biological pretreatment methods. Mainly chemical pretreatments (acid and alkali) were compared with biological pretreatment with lignolytic fungi Marasmiellus palmivorus PK-27. Structural and chemical changes as well as crystallinity of cellulose were examined through scanning electron microscopy, fourier transform infra red and X-ray diffraction analysis, respectively after pretreatment. Total reducing sugar released during enzymatic saccharification of pretreated substrates was also evaluated. Among the pretreatment methods, alkali (1 % NaOH) treated substrate showed high recovery of acid perceptible polymerised lignin (7.53 ± 0.5 mg/g) and significantly higher amount of reducing sugar (513.1 ± 41.0 mg/gds) compared to uninoculated Parthenium (163.4 ± 21.2) after 48 h of hydrolysis. This is the first report of lignolytic enzyme production from M. palmivorus, prevalent in oil palm plantations in Malaysia and its application in biological delignification of Parthenium sp. Alkali (1 % NaOH) treatment proves to be the suitable method of pretreatment for lignin recovery and enhanced yield of reducing sugar which may be used for bioethanol production from Parthenium sp.
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Acknowledgments
Authors are thankful to Post Graduate School and Director, Indian Agricultural Research Institute (New Delhi) for providing fellowship to carry out Ph.D. programme of first author and National Fund for Basic, Strategic and Frontier Application Research in Agriculture. We also thank Dr. V. V. Ramamurthy, Principal Scientist, Division of Entomology, Dr. Madhuban Gopal, National Fellow, Principal Scientist, Division of Agricultural Chemicals and Dr. S. C. Dutta, Principal Scientist, Division of Soil Science, IARI for providing SEM, FTIR and XRD facility, respectively.
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11274_2013_1422_MOESM1_ESM.docx
Plate shows the formation of bright red zone around the fungal disc producing lignolytic enzymes along with negative control (DOCX 1343 kb)
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Composition of reducing sugars in the enzymatic hydrolysates of different pretreatments was determined by HPLC (DOCX 12 kb)
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Pandiyan, K., Tiwari, R., Rana, S. et al. Comparative efficiency of different pretreatment methods on enzymatic digestibility of Parthenium sp.. World J Microbiol Biotechnol 30, 55–64 (2014). https://doi.org/10.1007/s11274-013-1422-1
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DOI: https://doi.org/10.1007/s11274-013-1422-1