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Endophytic bacteria isolated from orchid and their potential to promote plant growth

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Abstract

Twelve endophytic bacteria were isolated from the meristem of in vitro Cymbidium eburneum orchid, and screened according to indole yield quantified by colorimetric assay, in vitro phosphate solubilization, and potential for plant growth promotion under greenhouse conditions. Eight strains with positive results were classified into the genus Paenibacillus by FAME profile, and evaluated for their ability to increase survival and promote the growth of in vitro germinated Cattleya loddigesii seedlings during the acclimatization process. The obtained results showed that all strains produced detectable indole levels and did not exhibit potential for solubilizing inorganic phosphate. Particularly, an increase of the total biomass and number of leaves was observed. Two strains of Paenibacillus macerans promoted plant growth under greenhouse conditions. None of the treatments had a deleterious effect on growth of inoculated plants. These results suggest that these bacterial effects could be potentially useful to promote plant growth during seedling acclimatization in orchid species other than the species of origin.

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Acknowledgments

This study was supported by a grant from the State of São Paulo Research Foundation (FAPESP/BIOTA 2004/13910-6) and A.C.F. Dias (2008/54013-8) received a graduate fellowship.

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Authors and Affiliations

  1. Laboratory of Microbiology, University of Vale do Sapucaí, UNIVAS, Pouso Alegre, MG, Brazil

    Deise Cristina Faria

  2. Center for Nuclear Energy in Agriculture, University of Sao Paulo, Piracicaba, SP, Brazil

    Armando Cavalcante Franco Dias

  3. Laboratory of Environmental Microbiology, Embrapa Meio Ambiente, Jaguariuna, SP, Brazil

    Itamar Soares Melo

  4. Laboratory of Microbiology, Telecomunication National Institute, INATEL, Av. João de Camargo, Number 510, Santa Rita do Sapucaí, MG, CEP 37540-000, Brazil

    Francisco Eduardo de Carvalho Costa

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  1. Deise Cristina Faria
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  2. Armando Cavalcante Franco Dias
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  3. Itamar Soares Melo
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  4. Francisco Eduardo de Carvalho Costa
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Correspondence to Francisco Eduardo de Carvalho Costa.

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Faria, D.C., Dias, A.C.F., Melo, I.S. et al. Endophytic bacteria isolated from orchid and their potential to promote plant growth. World J Microbiol Biotechnol 29, 217–221 (2013). https://doi.org/10.1007/s11274-012-1173-4

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  • DOI: https://doi.org/10.1007/s11274-012-1173-4

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