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In vitro evaluation of some bacterial isolates as biofertilizers and biocontrol agents against the second stage juveniles of Meloidogyne incognita

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Abstract

Representative soil samples were collected from soil apart and the rhizosphere of different plant varieties grown in five Egyptian Governorates. The presence of plant parasitic nematodes (PPN) and free living nematodes (FLN) in the collected samples were estimated. In addition, one hundred and 65 bacterial cultures which well known as biofertilizers (some are able to fix nitrogen and the others solubilize either phosphate or potassium) were isolated. The isolated bacteria were screened depending on their rate of growth. Thirty-five cultures of fast growing nitrogen fixing bacteria (NFB), phosphate solubilizing bacteria (PSB) and potassium solubilizing bacteria (KSB) and their cultural filtrates were tested in vitro as biocontrol agents against the second stage juvenile (J2s) of the Meloidogyne incognita. In general, higher mortality percentages of nematodes were recorded by bacterial cultures than their comparative cultural filtrates. The highest mortality percentages were recorded by cultures of NFB7, PSB2 and KSB2 (100% at dilution 1/10), while it was 99.3, 99 and 97.8% at dilution 1/100, respectively. NFB7 exhibited a high nitrogen fixation rate (4.2 μmole N2/mL/h), while PSB2 and KSB2 effectively solubilized phosphate and potassium comparing with the control treatments (1.94 fold of available phosphate and 2.0 fold of available potassium, respectively). NFB7, PSB2 and KSB2 isolates showed the highest protease, gelatinase and chitinase activities which were thought responsible for their nematicidal effect. The three bacterial isolates were identified as Paenibacillus polymyxa, Bacillus megaterium and Bacillus circulans, respectively.

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Authors and Affiliations

  1. Department of Agricultural Microbiology, Faculty of Agriculture, Ain Shams University, Shoubra El-Kheima, Cairo, 11421, Egypt

    Muhammed E. El-Hadad, Magdi I. Mustafa, Shawky M. Selim & Tarek S. El-Tayeb

  2. Department of Plant Protection, Faculty of Agriculture, Ain Shams University, Shoubra El-Kheima, Cairo, 11421, Egypt

    Ahmed E. A. Mahgoob

  3. Central Laboratory for Agricultural Climate, Agricultural Research Center, Ministry of Agriculture, Giza, 12411, Egypt

    Norhan H. Abdel Aziz

Authors
  1. Muhammed E. El-Hadad
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  2. Magdi I. Mustafa
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  3. Shawky M. Selim
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  4. Ahmed E. A. Mahgoob
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  5. Tarek S. El-Tayeb
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  6. Norhan H. Abdel Aziz
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Correspondence to Tarek S. El-Tayeb.

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El-Hadad, M.E., Mustafa, M.I., Selim, S.M. et al. In vitro evaluation of some bacterial isolates as biofertilizers and biocontrol agents against the second stage juveniles of Meloidogyne incognita . World J Microbiol Biotechnol 26, 2249–2256 (2010). https://doi.org/10.1007/s11274-010-0413-8

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  • DOI: https://doi.org/10.1007/s11274-010-0413-8

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