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Identification of soybean herbivory-regulated genes and a transgenic investigation of their potential in insect resistance

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Abstract

Soybean (Glycine max) is an important economic crop worldwide that has been affected by common cutworm (Spodoptera litura Fabricius) in southern China. In this study, RNA-Seq was applied to extend our understanding of the functional complexity of the soybean transcriptome in response to insects. Totals of 1004 and 1580 unigenes were differentially expressed in resistant and susceptible soybean lines, respectively. The functional classification of these unigenes identified the same significant categories as in the previous microarray results, most of which were related to stress and defense responses. A qRT-PCR analysis of 21 selected genes confirmed the results of the RNA-Seq analysis. The integration of RNA-Seq, microarray and qRT-PCR data highlighted GmVSPβ and GmN:IFR as two candidate genes that may confer insect resistance. A functional analysis of GmVSPβ and GmN:IFR revealed a certain degree of resistance to common cutworm in overexpressing tobacco lines, and GmVSPβ was more efficient in this role, which may be associated with JA signaling regulation and nicotine biosynthesis. Taken together, our studies provide an overview and survey of the genes that are involved in insect resistance. Transgenic studies of two herbivory-regulated genes demonstrated their function in insect resistance and indicated their potential application to enhance insect resistance in crops.

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Acknowledgments

This work was supported in part by the Key Transgenic Breeding Program of China (2013ZX08004-003), National Natural Science Foundation of China (31201230, 31171573), Jiangsu Provincial Support Program (BK2012768, BE2012328), Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT13073) and Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP).

Author contribution statement

Yongli Wang designed and performed research, analyzed data and wrote the manuscript. Hui Wang and Qing Yang participated in the improvement of the manuscript. Yujie Ma participated in the insect bioassay of transgenic tobacco lines. Wenming Yang participated in the assessment of transgene copy numbers experiment. Deyue Yu designed the research and revised the manuscript.

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  1. National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China

    Yongli Wang, Hui Wang, Yujie Ma, Wenming Yang & Deyue Yu

  2. Biofuels Institute, School of the Environment, Jiangsu University, Zhenjiang, 212013, China

    Yongli Wang

  3. College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China

    Qing Yang

  4. College of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China

    Deyue Yu

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  1. Yongli Wang
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Wang, Y., Wang, H., Ma, Y. et al. Identification of soybean herbivory-regulated genes and a transgenic investigation of their potential in insect resistance. Plant Cell Tiss Organ Cult 123, 321–340 (2015). https://doi.org/10.1007/s11240-015-0837-9

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