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Haemostatic risk factors in dyslipidemic rabbits: role of 10-dehydrogingerdione as a new hypolipemic agent

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Abstract

Micro and macrovascular complications occurring during hyperlipidemia are mostly attributed to haemostatic impairment and vascular endothelial dysfunction. Cholesteryl ester transfer protein (CETP) inhibitors have been emerged recently as promising hypocholesterolemic agents to confer protection against lipid-mediated atherosclerosis. Therefore, 10-dehydrogingerdione (DHGD), a novel CETP inhibitor isolated from ginger rhizomes, was selected as a natural product in the present study to illustrate its effect on haemostatic impairment associated with hyperlipidemia as compared to a currently used hypocholesterolemic agent, atorvastatin (ATOR). Rabbits were fed a high cholesterol diet (HCD) and divided into three groups. One group served as control group while the other groups received DHGD or ATOR. Dyslipidemic rabbits showed a significant increase in serum endothelin-1, ischemia modified albumin, plasminogen activator inhibitor-1, prothrombin fragments (1+2) and plasma fibrinogen along with a decrease of nitric oxide level in serum. Daily administration of ATOR or DHGD significantly decreased the aforementioned coagulation and ischemia biomarkers and increased serum nitric oxide. DHGD (natural) results seem to be more remarkable as compared to ATOR (synthetic).

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Abbreviations

ABSU:

Absorbance units

ADMA:

Asymmetric dimethyl arginine

ANOVA:

Analysis of variance

ATOR:

Atorvastatin

CETP:

Cholesteryl ester transfer protein

CRP:

C-reactive protein

DHGD:

10-Dehydrogingerdione

ELISA:

Enzyme-linked immunosorbent assay

eNOS:

Endothelial nitric oxide synthase

ET-1:

Endothelin-1

FIB:

Fibrinogen

HCD:

High cholesterol diet

HDL-C:

High density lipoprotein cholesterol

IMA:

Ischemia modified albumin

LDL-C:

Low density lipoprotein cholesterol

NC:

Normal control

NO:

Nitric oxide

ox-LDL:

Oxidized low density lipoprotein

PAI-1:

Plasminogen activator inhibitor-1

PF1+2:

Prothrombin fragment (1+2)

ROS:

Reactive oxygen species

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Conflict of interest

The authors had no conflict of interest to disclose and declare that they did not receive any financial support for this work.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Pharmacy, Zagazig University, Sharkia Gov, Zagazig, 44519, Egypt

    Mohamed Mahmoud El-Seweidy, Mervat El-Sayed Asker, Hebatallah Husseini Atteia & Mohamed Ahmed Abdallah

  2. Department of Phramacognosy, Faculty of Pharmacy, Zagazig University, Sharkia Gov, Zagazig, Egypt

    Sameih Ibrahim Eldahmy

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  1. Mohamed Mahmoud El-Seweidy
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  2. Mervat El-Sayed Asker
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Correspondence to Hebatallah Husseini Atteia.

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El-Seweidy, M.M., Asker, M.ES., Eldahmy, S.I. et al. Haemostatic risk factors in dyslipidemic rabbits: role of 10-dehydrogingerdione as a new hypolipemic agent. J Thromb Thrombolysis 39, 196–202 (2015). https://doi.org/10.1007/s11239-014-1150-x

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