Abstract
“Pichi” or “pichi romero” (Fabiana imbricata R. et. P., Solanaceae) is a Chilean plant used as a tea in the Andean regions of Chile and Argentina. A very simple and direct method was developed for the qualitative analysis of polyphenols in the tea by high-performance liquid chromatography (HPLC) with diode array detection and electrospray ionization tandem mass spectrometry. The phenolic constituents identified in the teas were chlorogenic acid (3-O-caffeoylquinic acid), p-hydroxyacetophenone, scopoletin and quercetin derivatives. The glycosides were mainly glucosides from p-hydroxyacetophenone and scopoletin while di- and tri-glycosides from quercetin were the main flavonoids. The content of the main phenolic compounds in the teas (g/100 g lyophilized infusion) was 0.8–1.9 % for scopoletin, 0.4–6.2 % for p-hydroxyacetophenone and 2.1–4.3 % for rutin, respectively. The health-promoting properties reported for this herbal tea can be associated with the presence of several phenolics with known antioxidant, diuretic and antiinflammatory activity.
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Abbreviations
- HPLC-DAD:
-
High-performance liquid chromatography with diode array detection
- HPLC-MS:
-
High-performance liquid chromatography mass spectrometry
- HPLC-DAD-ESI-MS:
-
High-performance liquid chromatography with diode array detection electrospray ionization mass spectrometry
- CID:
-
Collision induced dissociation
- LC/MS:
-
Liquid chromatography – mass spectrometry
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Acknowledgments
Financial support by Programa de Investigación en Productos Bioactivos, Universidad de Talca is kindly acknowledged. Cristina Quispe and Ezequiel Viveros Valdez thank the PBCT Program, PSD-50 for a postdoctoral grant.
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Quispe, C., Viveros-Valdez, E. & Schmeda-Hirschmann, G. Phenolic Constituents of the Chilean Herbal Tea Fabiana imbricata R. et P.. Plant Foods Hum Nutr 67, 242–246 (2012). https://doi.org/10.1007/s11130-012-0302-4
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DOI: https://doi.org/10.1007/s11130-012-0302-4