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Over-Expression of PmHSP17.9 in Transgenic Arabidopsis thaliana Confers Thermotolerance

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Abstract

Small heat shock proteins (sHSPs) have been shown to be involved in stress tolerance. However, their functions in Prunus mume under heat treatment are poorly characterized. To improve our understanding of sHSPs, we cloned a sHSP gene, PmHSP17.9, from P. mume. Sequence alignment and phylogenetic analysis indicated that PmHSP17.9 was a member of plant cytosolic class III sHSPs. Besides heat stress, PmHSP17.9 was also upregulated by salt, dehydration, oxidative stresses and ABA treatment. Leaves of transgenic Arabidopsis thaliana that ectopically express PmHSP17.9 accumulated less O2 − and H2O2 compared with wild type (WT) after 42 °C treatment for 6 h. Over-expression of PmHSP17.9 in transgenic Arabidopsis enhanced seedling thermotolerance by decreased relative electrolyte leakage and MDA content under heat stress treatment when compared to WT plants. In addition, the induced expression of HSP101, HSFA2, and delta 1-pyrroline-5-carboxylate synthase (P5CS) under heat stress was more pronounced in transgenic plants than in WT plants. These results support the positive role of PmHSP17.9 in response to heat stress treatment.

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Acknowledgments

This work was supported by the grants from the National 863 Project of China (No. 2011AA100207) and the National Natural Science Foundation of China (No. 31270739). We thank all the colleagues in our laboratory for constructive discussion and technical support.

Authors’ Contributions

WXL performed the experiments and wrote the manuscript; YJ, LXB and ZQ helped perform the experiments; GC helped design the experiment; ZJW and BMZ designed the experiments and reviewed the manuscript. All authors have read and approved the final manuscript and have no competing interests.

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  1. Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Xue-Li Wan, Jie Yang, Xiao-Bai Li, Qiao Zhou, Cong Guo, Man-Zhu Bao & Jun-Wei Zhang

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  1. Xue-Li Wan
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Correspondence to Jun-Wei Zhang.

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Wan, XL., Yang, J., Li, XB. et al. Over-Expression of PmHSP17.9 in Transgenic Arabidopsis thaliana Confers Thermotolerance. Plant Mol Biol Rep 34, 899–908 (2016). https://doi.org/10.1007/s11105-016-0974-2

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