Abstract
Apple (Malus × domestica Borkh.) is a typical Rosaceae species that exhibits gametophytic self-incompatibility (GSI) controlled by polymorphic S-alleles. In this study, the S-alleles of wild Malus species were amplified, sequenced and compared using polymerase chain reaction (PCR) technology. 21 S-alleles were identified in 27 wild Malus species. The results indicated that the overwhelming majority of S-alleles between wild Malus species and cultivars shared identical sequences. Simultaneously, five new S alleles (designated S 48 –S 52 ) were identified in wild Malus species. There are the S 48 -RNase in M. angustifolia (Ation) Michaux, S 49 -RNase in M. orientalis Uglitzk. Ex Juz. and M. sylvestris (L.) Mill., S 50 -RNase in M. tschonoskii (Maxim.) C.K. Schneid. and M. sieversii (Ldb.) Roem., S 51 -RNase in M. komarovii (Sarg.) Rehd. and M. kansuensis (Batal.) C. K. Schneid., S 52 -RNase in M. manshurica (Maxim.) V. Komorov wild Malus species. Additionally, an S 1 -RNase was cloned in wild Malus prunifolia var. ringo, which have the same open reading frame as Malus × domestica cv. Fuji, but lacked whole intron.
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Acknowledgements
This work was supported by the National Natural Science Foundation (No. 30871697, 31071784) and Beijing Natural Science Foundation of China (No. 6102017). We also thank the Institute of Pomology of the Liaoning Academy of Agricultural Sciences and Fruit Tree Research Institute of the Chinese Academy of Agricultural Sciences for providing plant materials.
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Li, T., Long, S., Li, M. et al. Determination S-Genotypes and Identification of Five Novel S-RNase Alleles in Wild Malus Species. Plant Mol Biol Rep 30, 453–461 (2012). https://doi.org/10.1007/s11105-011-0345-y
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DOI: https://doi.org/10.1007/s11105-011-0345-y