Abstract
In sweet cherry (Prunus avium L.), theS4′ haplotype, characterized by a self-incompatibility (SI) defect in pollen, is self-compatible and is derived from the self-incompatibleS4 haplotype by x-ray mutagenesis.SFBs (S haplotype-specific F-box protein genes) have been found to associate with pollen determinant of SI. This report identified theSFB4′ of the self-compatibleS4′ haplotype. The alignment of the sequences ofSFB4′ andSFB4 by the BLAST program revealed a 4-bp deletion inSFB4′, which is TTTA. The sequence polymorphism generated by the TTTA deletion inSFB4′ was exploited to develop a simple molecular marker specific for detecting theS4′ but not theS4 haplotype. The simple marker specific to theS4′ haplotype can be visualized directly on an agarose gel, so it can be immediately applied to a marker-assistant cherry-breeding program. Thus, this work provides a practical molecular marker for cherry breeding.
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Abbreviations
- AS-PCR:
-
allele-specific PCR
- CTAB:
-
cetyltrimethylammonium bromide
- dCAPS:
-
derived cleaved amplified polymorphic sequences
- SFB :
-
S haplotype-specific F-box protein gene
- SI:
-
self-incompatibility
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An erratum to this article is available at http://dx.doi.org/10.1007/BF02772655.
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Zhu, M., Zhang, X., Zhang, K. et al. Development of a simple molecular marker specific for detecting the self-compatibleS4′ haplotype in sweet cherry (Prunus avium L.). Plant Mol Biol Rep 22, 387–398 (2004). https://doi.org/10.1007/BF02772681
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DOI: https://doi.org/10.1007/BF02772681