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Transcriptional response of Pseudomonas aeruginosa to a phosphate-deficient Lolium perenne rhizosphere

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Abstract

Background and aims

Plant-bacterial interactions in the rhizosphere are important in mediating soil nutrient transformations. Plants supply carbon-rich substrates to rhizobacteria as root exudates and bacteria mobilise soil-bound phosphate for plant nutrition. This study aimed to probe the specificity of the plant effect on bacterial gene expression in P-starved rhizosphere conditions.

Methods

DNA microarrays were employed to study gene expression in the rhizosphere of Lolium perenne grown under high and low phosphate regimes (330 μM vs. 3–6 μM phosphate). Root exudation under these regimes was also quantified. Phosphate-regulated gene expression of a panel of 22 genes was compared in rhizosphere, planktonic culture and during biofilm growth on an artificial root.

Results

Plant growth and root exudation were affected by P-availability. P-limited conditions induced increased expression of bacterial genes of an aromatic degradation pathway (catA), heavy metal sensing (PA2523), and membrane proteins (glpM, crcB), while genes involved in cell motility and amino acid uptake/ metabolism were downregulated. A crcB mutant was impaired in rhizosphere survival under low phosphate conditions, though glpM and catA mutants were not affected. Several of the genes studied were induced by phosphate limitation in all three lifestyles studied.

Conclusions

Our results show the importance of the plant-microbe interaction in controlling the bacterial transcriptional response in a phosphate-limited rhizosphere.

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Acknowledgements

This work was supported by the Natural Environment Research Council (NERC) and by a CASE studentship to AZ sponsored by the Biotechnology and Biological Sciences Research Council (BBSRC) and Macaulay Enterprises Ltd.

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  1. Hervé Sanguin

    Present address: CIRAD, UMR 113 CIRAD/INRA/IRD/SUP-AGRO/UM2, Laboratoire des Symbioses Tropicales et Mediterraneennes (LSTM), TA A-8/J, Campus International de Baillarguet, Montpellier, F-34398, France

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  1. Faculty of Life Sciences, University of Manchester, Oxford Rd, Manchester, M13 9PT, UK

    Agnieszka Zyśko, Hervé Sanguin, Andrew Hayes, Leanne Wardleworth, Leo A. H. Zeef & Michael A. Kertesz

  2. The James Hutton Institute, Craigiebuckler, Aberdeen, AB15 8QH, Scotland, UK

    Allan Sim, Eric Paterson & Brajesh K. Singh

  3. Hawkesbury Institute for the Environment, University of Western Sydney, Locked Bag 1797, Penrith South, NSW, 1797, Australia

    Brajesh K. Singh

  4. Faculty of Agriculture, Food and Natural Resources, The University of Sydney, Sydney, NSW, 2006, Australia

    Michael A. Kertesz

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Zyśko, A., Sanguin, H., Hayes, A. et al. Transcriptional response of Pseudomonas aeruginosa to a phosphate-deficient Lolium perenne rhizosphere. Plant Soil 359, 25–44 (2012). https://doi.org/10.1007/s11104-011-1060-z

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