Abstract
A wide range of molecules are transported across membranes by the ATP binding cassette (ABC) transporters. Plants possess a collection of ABC proteins bearing similarities to the components of prokaryotic multi subunit ABC transporters, designed as ABC group I. However the functions of most of them are not well understood. Here, we characterized a naturally occurring rice mutant that exhibited albino phenotype under continuous rainy days in the field, but gradually recovered to normal green after the rainy season. Molecular and genetic analyses revealed that the phenotypes were caused by a mutation in the OsABCI8 that encoded a member of the ABCI family. Subcellular localization demonstrated that OsABCI8 is a chloroplast ABC transporter. Expression of OsABCI8 is significantly enhanced in rainy days compared to sunny days. Besides defects in chloroplast development and chlorophyll biosynthesis, the mutant phenotype is accompanied by a higher accumulation of iron, suggesting that OsABCI8 is involved in iron transportation and/or homeostasis in rice. Our results demonstrate that OsABCI8 represents a conserved ABCI protein involved in transition metals transportation and/or homeostasis and suggest an important role of the plastid-localized OsABCI8 for chloroplast development.
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Acknowledgements
We thank the National Center of Plant Gene Research (Huazhong Agricultural University, China) for providing the T-DNA insertion line, ATL_03Z11JN90_LBT2, for the OsABCI8 gene. This work was supported by the National Natural Science Foundation of China (Grants Nos. 31671594 to XQ. Zhang and 31272491 to XM. Xie), the Natural Science Foundation of Anhui Province, China (Grant No. 1508085MC46 to B. Teng), Natural Science Foundation of Guangdong Province, China (Grant Nos. 2014A030313457 and 2015A020209118 to XQ. Zhang).
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The author(s) have made the following declarations about their contributions: Conceived and designed the experiments: XQZ TFH. Performed the experiments: XZ, RT, HG and SK. Analyzed the data: BT YHH XZ. Contributed reagents/materials/analysis tools: ZX XMX. Wrote the paper: XQZ TFH. All authors discussed the results and commented on the manuscript.
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Xiuyu Zeng and Ran Tang have contributed equally to this work.
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Zeng, X., Tang, R., Guo, H. et al. A naturally occurring conditional albino mutant in rice caused by defects in the plastid-localized OsABCI8 transporter. Plant Mol Biol 94, 137–148 (2017). https://doi.org/10.1007/s11103-017-0598-4
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DOI: https://doi.org/10.1007/s11103-017-0598-4