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Apigenin Mediated Protection of OGD-Evoked Neuron-Like Injury in Differentiated PC12 Cells

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Abstract

Ischemic stroke has been confirmed to cause neuronal injury due to its insufficient supply of glucose and oxygen to brain tissue. Previous research has shown that oxidative stress, a result of excessive accumulation of reactive oxygen species (ROS), relates to pathophysiology of ischemic stroke, and causes oxidative damage to biomolecules, eventually leading to programmed cell death. Meanwhile, apigenin has been shown to exhibit antioxidant, anti-inflammatory, anti-cancer properties and neuroprotective action. Hence, this study was to investigate the potential mechanisms underlying the neural protection of apigenin on oxygen and glucose deprivation/reperfusion (OGD/R) induced neuronal injury in differentiated PC12 cells. Cells were pretreated with apigenin for 6 h, and then subjected to OGD for 12 h followed by reperfusion for 24 h. The results showed that OGD/R significantly decreased cell viability, mitochondrial membrane potential, mRNA levels of antioxidant and detoxifying enzymes and Nrf2 protein expression, while elevated the release of LDH, cell apoptosis, intracellular ROS level, P53 protein expression and upregulated its downstream genes in PC12 cells. However, apigenin effectively inhibited these undesirable changes induced by OGD/R. Our findings demonstrate that this compound attenuates OGD/R induced neuronal injury mainly by virtue of its anti-apoptosis and antioxidative properties via affecting the expression of Nrf2 and P53, and their downstream target gene transcription.

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Acknowledgments

The work was supported by Guangdong Provincial International Cooperation Project Funding of People’s Republic of China (No. 2011B050300021).

Conflict of interest

The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, People’s Republic of China

    Huizhen Guo, Weimin Chen, Zhenhua Dai, Tongxiang Lin, Jiyan Su, Shanshan Li, Qingfeng Xie & Yang Xu

  2. School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, People’s Republic of China

    Huizhen Guo, Songzhi Kong, Jiyan Su, Ziren Su & Xiaoping Lai

  3. College of Science, Guangdong Ocean University, Zhanjiang, Guangdong, People’s Republic of China

    Songzhi Kong

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  1. Huizhen Guo
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  10. Yang Xu
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Correspondence to Yang Xu or Xiaoping Lai.

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Huizhen Guo and Songzhi Kong have contributed equally to this work.

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Guo, H., Kong, S., Chen, W. et al. Apigenin Mediated Protection of OGD-Evoked Neuron-Like Injury in Differentiated PC12 Cells. Neurochem Res 39, 2197–2210 (2014). https://doi.org/10.1007/s11064-014-1421-0

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