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A Differential Proteomic Approach Reveals an Evolutionary Conserved Regulation of Nme Proteins by Fe65 in C. elegans and Mouse

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Abstract

The function of the APP–Fe65 complex is still not definitively understood. To address this point we studied the phenotype of Fe65 (feh-1) ablation, which results in severe developmental defects in C. elegans, including embryonic and larval arrests. To shed light on the complex phenotype of embryonic arrest, we undertook a systematic approach, aiming at the definition of the altered proteomic profile of feh-1 null worms. We defined a panel of 27 regulated proteins, 16 of which actually participating to embryonic development processes in the nematode. Protein spots corresponding to the products of the F25H2.5 gene, the nematode orthologue of mammalian Nm23/Nme gene family members, were consistently up-regulated in feh-1 −/− embryos. We observed similar up-regulation of Nme1 and Nme2 genes, both at the transcript and the protein levels, in the brain of Fe65 knock-out mice, thus highlighting the occurrence of evolutionary conserved mechanisms of Nme expression in nematodes and mammals.

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Acknowledgments

The author’s work is supported by MiUR-PRIN 2005 Programmes (to N.Z. and T.R.), Regione Campania (L.R. n. 5), 6th Framework Programme, EU (APOPIS) to T.R. We thank the Centro Regionale di Competenza GEAR (Genomics for Applied Research) for 2D-DIGE facility, and M. Zollo for helpful discussion.

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Authors and Affiliations

  1. CEINGE Biotecnologie Avanzate, Via Comunale Margherita, 482, 80145, Napoli, Italy

    Francesco Napolitano, Fulvio D’Angelo, Marida Bimonte, Valeria Perrina, Tommaso Russo & Nicola Zambrano

  2. Dipartimento di Biochimica e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, 80131, Napoli, Italy

    Francesco Napolitano, Fulvio D’Angelo, Marida Bimonte, Valeria Perrina, Tommaso Russo & Nicola Zambrano

  3. Proteomics and Mass Spectrometry Laboratory, ISPAAM, Consiglio Nazionale delle Ricerche, 80147, Napoli, Italy

    Chiara D’Ambrosio & Andrea Scaloni

Authors
  1. Francesco Napolitano
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  2. Fulvio D’Angelo
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  3. Marida Bimonte
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  4. Valeria Perrina
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  5. Chiara D’Ambrosio
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  6. Andrea Scaloni
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  7. Tommaso Russo
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  8. Nicola Zambrano
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Correspondence to Tommaso Russo or Nicola Zambrano.

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Special issue article in honor of Dr. Anna Maria Giuffrida-Stella.

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Napolitano, F., D’Angelo, F., Bimonte, M. et al. A Differential Proteomic Approach Reveals an Evolutionary Conserved Regulation of Nme Proteins by Fe65 in C. elegans and Mouse. Neurochem Res 33, 2547–2555 (2008). https://doi.org/10.1007/s11064-008-9683-z

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  • DOI: https://doi.org/10.1007/s11064-008-9683-z

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