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Dormancy release of Cotinus coggygria seeds under a pre-cold moist stratification: an endogenous abscisic acid/gibberellic acid and comparative proteomic analysis

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Abstract

Seed dormancy is an adaptive trait that widely exists in angiosperms and gymnosperms. The mechanisms for the release of seed dormancy have been less well studied. Using smoke tree (Cotinus coggygria var. Cinerea Engler) seeds, the effect of cold moist stratification (5 °C, 18.5 % humidity and 0–75 days) on dormancy release, changes of respiration rate, ABA and GA3 content, and the differentially expressed proteins during dormancy release were investigated. Seed dormancy was released during cold moist stratification, seed respiration rate was increased while both ABA and GA3 concentrations were decreased. A total of 28 protein spots with significant changes in relative expression abundance were detected by two-dimensional electrophoresis. Among these protein spots, four proteins of ATPase β subunit, heat-shock cognate protein 70, aspartic proteinase 1 and actin were successfully identified by the matrix assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry. These four proteins were all down-expressed during dormancy release, and their possible implications for dormancy release of C. coggygria seeds was discussed. A better understanding of seed dormancy release and germination has practical benefits to seedling production for forest regeneration purposes.

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Acknowledgments

We are grateful to Drs. Shihua Shen, Xue Zhao and Kuixian Ji from the Institute of Botany, Chinese Academy of Sciences, Beijing, China and Dr. Langtao Xiao, Ms. Jianhua Tong and Qiong Peng from the Hunan Provincial Key Laboratory of Phytohormone and Growth Development, Hunan Agricultural University, Changsha, Hunan, China for providing assistances; and the National Natural Science Foundation of China (81303169), the Doctoral Scientific Research Foundation of Hubei University for Nationalities (MY2013B014), Guangxi Natural Science Foundation (2013jjBA40121) and the Open Fund of Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province (PKLHB1304) for financial support.

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Authors and Affiliations

  1. Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province, Enshi, Hubei Province, China

    Zhi Jun Deng

  2. Seed Biology Laboratory, College of Forestry and Horticulture, Hubei University for Nationalities, Enshi, Hubei Province, China

    Zhi Jun Deng, Xiao Fang Hu, Xun Ru Ai, Lan Yao, Shi Ming Deng & Xin Pu

  3. Guangxi Botanical Garden of Medicinal Plant, Nanning, 530023, Guangxi Zhuang Autonomic Region, China

    Zhi Jun Deng

  4. Group of Seed Physiology and Biotechnology, Institute of Botany, The Chinese Academy of Sciences, Beijing, China

    Song Quan Song

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  1. Zhi Jun Deng
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Correspondence to Zhi Jun Deng.

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Deng, Z.J., Hu, X.F., Ai, X.R. et al. Dormancy release of Cotinus coggygria seeds under a pre-cold moist stratification: an endogenous abscisic acid/gibberellic acid and comparative proteomic analysis. New Forests 47, 105–118 (2016). https://doi.org/10.1007/s11056-015-9496-2

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