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Phenotyping at hot spots and tagging of QTLs conferring spot blotch resistance in bread wheat

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Abstract

Spot blotch is a major foliar disease of wheat caused by Bipolaris sorokiniana in warm and humid environments of the world including South Asian countries. In India, it has a larger impact in Indo-Gangetic plains of the country. Therefore, the present study was undertaken to phenotype a mapping population at different hot spots of India and to detect quantitative trait loci (QTL) for resistance to spot blotch in wheat. For this study, 209 single seed descent (SSD) derived F8, F9, F10 recombinant inbred lines (RILs) of the cross ‘Sonalika’ (an Indian susceptible cultivar)/‘BH 1146’ (a Brazilian resistant cultivar) were assessed for spot blotch resistance at two hot spot locations (Coochbehar and Kalyani) for three years and for two years under controlled conditions in the polyhouse (Karnal). The population showed large variation in spot blotch reaction for disease severity in all the environments indicating polygenic nature of the disease. Microsatellite markers were used to create the linkage maps. Joint and/or individual year analysis by composite interval mapping (CIM) and likelihood of odds ratio (LOD) >2.1, detected two consistent QTLs mapped on chromosome 7BL and 7DL and these explained phenotypic variation of 11.4 percent and 9.5 percent over the years and locations, respectively. The resistance at these loci was contributed by the parent ‘BH 1146’ and shown to be independent of plant height and earliness. Besides, association of some agro-morphological traits has also been observed with percent disease severity. These identified genomic regions may be used in future wheat breeding programs through marker assisted selection for developing spot blotch resistant cultivars.

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Acknowledgments

The authors thankfully acknowledge the financial support from Department of Biotechnology (DBT), New Delhi, India for the present study. The first author is also grateful to University Grants Commission (UGC), New Delhi, India for providing financial support in form of UGC fellowship to carry out doctoral degree.

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Authors and Affiliations

  1. Indian Institute of Wheat and Barley Research, Karnal, India

    Virender Singh, Gyanendra Singh, Ashish Ojha, B. S. Tyagi & Sonia Sheoran

  2. Uttar Banga Krishi Viswavidyalaya, Pundibari, Coochbehar, India

    A. K. Chowdhary

  3. Department of Bio and Nano Technology, Bio and Nano Technology Centre, Guru Jambheshwar University of Science and Technology, Hisar, India

    Virender Singh & A. Chaudhury

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  1. Virender Singh
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  2. Gyanendra Singh
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Correspondence to Sonia Sheoran.

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Singh, V., Singh, G., Chaudhury, A. et al. Phenotyping at hot spots and tagging of QTLs conferring spot blotch resistance in bread wheat. Mol Biol Rep 43, 1293–1303 (2016). https://doi.org/10.1007/s11033-016-4066-z

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  • DOI: https://doi.org/10.1007/s11033-016-4066-z

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