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Profiling of mitochondrial proteome in wheat roots

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Abstract

Mitochondria are important organelles for cellular respiration within the eukaryotic cell and have many important functions including vitamin synthesis, amino acid metabolism and photorespiration. To investigate the mitochondrial proteome of the roots of wheat seedlings, a systematic and targeted analysis were carried out on the mitochondrial proteome from 15 day-old wheat seedling root material. Mitochondria were isolated by Percoll gradient centrifugation; and extracted proteins were disassociated and analyzed by Tricine SDS-PAGE couple to LTQ–FTICR mass spectrometry. From the isolated the sample, 184 proteins were identified which is composed of 140 proteins as mitochondria and 44 proteins as other subcellular proteins that are predicted by the freeware sub-cellular predictor. The identified proteins in mitochondria were functionally classified into 12 classes using the ProtFun 2.2 servers based on biological processes. Proteins were shown to be involved in amino acid biosynthesis (17.1 %), biosynthesis of cofactors (6.4 %), cell envelope (11.4 %), central intermediary metabolism (10 %), energy metabolism (20 %), fatty acid metabolism (0.7 %), purines and pyrimidines (5.7 %), regulatory functions (0.7 %), replication and transcription (1.4 %), translation (22.1 %), transport and binding (1.4 %), and unknown (2.8 %). These results indicate that many of the protein components present and functions of identifying proteins are common to other profiles of mitochondrial proteins performed to date. These results are provided the extensive and noble clues, to our knowledge, of mitochondrial proteins from wheat roots.

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Abbreviations

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

IEF:

Isoelectric focusing

pI :

Isoelectric point

FT:

Fourier transform

LTQ:

Linear quadruple trap

ICR:

Ion cyclotron resonance

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Acknowledgments

This work was supported by a grant from the AGENDA (9069532012), RDA, Korea to S. H. Woo, College of Agriculture, Life and Environments, Chungbuk National University, Korea.

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Author notes

  1. Abu Hena Mostafa Kamal

    Present address: National Institute of Crop Science, NARO, Tsukuba, 305-8518, Japan

Authors and Affiliations

  1. Department of Crop Science, Chungbuk National University, 410 Seongbong-ro, Heungdeok-gu, Cheongju, Chungbuk, 361-763, Korea

    Da-Eun Kim, Swapan Kumar Roy, Abu Hena Mostafa Kamal, Soo Jeong Kwon & Sun-Hee Woo

  2. Division of Mass Spectrometry Research, Korea Basic Science Institute, Ochang, Chungbuk, 863-883, Korea

    Kun Cho

  3. Laboratory of Molecular Breeding, Arid Land Research Center, Tottori University, Tottori, 680-8550, Japan

    Seong-Woo Cho

  4. Department of Crop, Agriculture and Life Science, Chonbuk National University, Jeonju, 561-756, Korea

    Chul-Soo Park

  5. Division of Life Science, Korea Basic Science Institute, Daejeon, 305-333, Korea

    Jong-Soon Choi

  6. National Institute of Crop Science, NARO, Tsukuba, 305-8518, Japan

    Setsuko Komatsu

  7. Department of Industrial Plant Science & Technology, Chungbuk National University, Cheongju, 361-763, Korea

    Moon-Soon Lee

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  1. Da-Eun Kim
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Corresponding authors

Correspondence to Moon-Soon Lee or Sun-Hee Woo.

Additional information

Da-Eun Kim, Swapan Kumar Roy, Abu Hena Mostafa Kamal have contributed equally to this article.

Electronic supplementary material

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11033_2014_3407_MOESM1_ESM.xls

Supplementary Table 1. List of identifying mitochondrial proteins in roots of wheat seedlings by Tricine SDS-PAGE, which is analyzed by LTQ-FT-ICR mass spectrometry (XLS 70 kb)

11033_2014_3407_MOESM2_ESM.xls

Supplementary Table 2. List of identifying proteins from other organelles as contaminant proteins in wheat roots (XLS 40 kb)

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Kim, DE., Roy, S.K., Kamal, A.H.M. et al. Profiling of mitochondrial proteome in wheat roots. Mol Biol Rep 41, 5359–5366 (2014). https://doi.org/10.1007/s11033-014-3407-z

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  • DOI: https://doi.org/10.1007/s11033-014-3407-z

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