Abstract
The performance of control and water-stressed 10-d-old wheat seedlings was compared. During short-term water stress (irrigation was withheld for 9 d), rates of photosynthesis and transpiration, stomatal conductance, and relative water content decreased whereas the proline content increased. Chloroplast proteins were extracted from the leaves, separated by iso-electric focusing through two-dimensional electrophoresis, and stained with CBB R-250. Differentially expressed proteins were detected and analyzed with MALDI-TOF/TOF mass spectrometry. Under water stress, 9 proteins were up-regulated whereas 11 proteins were not affected. The ribulose-1,5-bisphospate carboxylase/oxygenase (Rubisco) small and large subunits, chloride carrier/channel family, and H+-ATPase were up-regulated by water stress whereas membrane-bound ATP synthase subunit b and cytochrome b6-f complex were down-regulated.
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Abbreviations
- 2-DE:
-
two-dimensional electrophoresis
- CLC:
-
chloride carrier/channel
- MALDI:
-
matrix assisted laser desorption/ionization
- Rubisco:
-
ribulose-1,5-bisphospate carboxylase/oxygenase
- TOF:
-
time-of-flight
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Acknowledgements: Financial support for this study was obtained from Agenda project (PJ9069532012), RDA, Suwon, Kyonggi, Korea to S. H. Woo, and the project fund (C32730) to J.S. Choi from the Center for Analytical Research of Disaster Science of Korea Basic Science Institute, and also technically supported by the Korea Basic Science Institute Research Grant (T32608) to K.Cho. A.H.M. Kamal and K. Cho equally contributed to this article.
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Kamal, A.H.M., Cho, K., Choi, J.S. et al. Patterns of protein expression in water-stressed wheat chloroplasts. Biol Plant 57, 305–312 (2013). https://doi.org/10.1007/s10535-012-0290-0
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DOI: https://doi.org/10.1007/s10535-012-0290-0