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Association of SCD1 and DGAT1 SNPs with the intramuscular fat traits in Chinese Simmental cattle and their distribution in eight Chinese cattle breeds

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An Erratum to this article was published on 14 March 2013

Abstract

Intramuscular fat (IMF) is a key parameter for evaluation of nutritional quality of beef, with its endogenous synthesis regulated by stearoyl CoA desaturase (SCD1) and diacylglycerol-acyl transferase 1 (DGAT1) genes in cattle. The object of this research was to evaluate the effect of SCD1 and DGAT1 polymorphisms on IMF trait in beef cattle and to estimate the frequency distribution of SNPs in the two genes in Chinese cattle populations. The SCD1 and DGAT1 polymorphisms were detected by PCR-single strand conformation polymorphism (PCR-SSCP) method in Chinese Simmental cattle and their associations with IMF traits were analyzed using the general linear model (GLM). The frequency distribution of SNPs in SCD1 and DGAT1 genes were detected by PCR-SSCP method and analyzed in seven other cattle populations. The results showed significant associations of SNPs SCD1-878, SCD1-762, and DGAT1 10433 and 10434 with IMF (%) and shearing force values (SFV; kg) in Chinese Simmental cattle. A haplotype combining SCD1-878C, SCD1-762T, and DGAT1 10433 and 10434-GC had the highest IMF, marbling score and shearing force. The polymorphic investigation indicated that the frequency of SCD1-878C or SCD1-762T was significantly higher in Chinese southern cattle (Leiqiong, Yunnan High pump, BMY or Minnan Cattle) than in Chinese northern cattle (Chinese Simmental, Luxi Cattle, Bohai Black or Chinese Holstein), while the frequency of DGAT1 10433 and 10434-GC in Chinese indigenous breed (Leiqiong, Yunnan High pump, BMY, Luxi Cattle, Bohai Black, or Minnan Cattle) was significantly lower than breeds with imported blood (Chinese Simmental or Chinese Holstein). These findings demonstrated that both the SCD1 and DGAT1 SNPs were prospect genetic markers for IMF traits, and the SCD1 SNPs could be used as a genetic marker for southern or northern blood in Chinese cattle.

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Acknowledgment

This project was supported by National Plan of Scientific and Technological Support (2008BADB2B03-06), 863 Key Projects (2008AA101010). We thank Dr. Hou G.Y. and Mr. Huang B.Z. for helpful comments and providing us with blood samples for the research.

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Authors and Affiliations

  1. Key Lab of Animal Genetics, Breeding and Molecular Design of Jangsu Province, College of Animal Science and Technology Yangzhou University, Yangzhou, 225009, People’s Republic of China

    X. X. Wu, Z. P. Yang, X. K. Shi, D. J. Ji, Y. J. Mao & L. L. Chang

  2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, People’s Republic of China

    J. Y. Li, D. J. Ji & H. J. Gao

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  1. X. X. Wu
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  2. Z. P. Yang
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Correspondence to Z. P. Yang or J. Y. Li.

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An erratum to this article can be found online at http://dx.doi.org/10.1007/s11033-013-2489-3.

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Wu, X.X., Yang, Z.P., Shi, X.K. et al. Association of SCD1 and DGAT1 SNPs with the intramuscular fat traits in Chinese Simmental cattle and their distribution in eight Chinese cattle breeds. Mol Biol Rep 39, 1065–1071 (2012). https://doi.org/10.1007/s11033-011-0832-0

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