Skip to main content
SpringerLink
Log in

Molecular mapping of a new temperature-sensitive gene LrZH22 for leaf rust resistance in Chinese wheat cultivar Zhoumai 22

  • Published:
Molecular Breeding Aims and scope Submit manuscript

Abstract

Leaf rust, caused by Puccinia triticina, is one of the most widespread diseases in common wheat globally. The Chinese wheat cultivar Zhoumai 22 is highly resistant to leaf rust at the seedling and adult stages. Seedlings of Zhoumai 22 and 36 lines with known leaf rust resistance genes were inoculated with 13 P. triticina races for gene postulation. The leaf rust response of Zhoumai 22 was different from those of the single gene lines. With the objective of identifying and mapping, the new gene(s) for resistance to leaf rust, F1, F2 plants and F2:3 lines from the cross Zhoumai 22/Chinese Spring were inoculated with Chinese P. triticina race FHDQ at the seedling stage. A single dominant gene, tentatively designated LrZH22, conferred resistance. To identify other possible genes in Zhoumai 22, ten P. triticina races avirulent on Zhoumai 22 were used to inoculate 24 F2:3 lines. The same gene conferred resistance to all ten avirulent races. A total of 1300 simple sequence repeat (SSR) markers and 36 EST markers on 2BS were used to test the parents, and resistant and susceptible bulks. Resistance gene LrZH22 was mapped in the chromosome bin 2BS1-0.53-0.75 and closely linked to six SSR markers (barc183, barc55, gwm148, gwm410, gwm374 and wmc474) and two EST markers (BF202681 and BE499478) on chromosome arm 2BS. The two closest flanking SSR loci were Xbarc55 and Xgwm374 with genetic distances of 2.4 and 4.8 cM from LrZH22, respectively. Six designated genes (Lr13, Lr16, Lr23, Lr35, Lr48 and Lr73) are located on chromosome arm 2BS. In seedling tests, LrZH22 was temperature sensitive, conferring resistance at high temperatures. The reaction pattern of Zhoumai 22 was different from that of RL 4031 (Lr13), RL 6005 (Lr16) and RL 6012 (Lr23), Lr35 and Lr48 are adult-plant resistance genes, and Lr73 is not sensitive to the temperature. Therefore, LrZH22 is likely to be a new leaf rust resistance gene or allele.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Bansal UK, Hayden MJ, Venkata BP, Khanna R, Saini RG, Bariana HS (2008) Genetic mapping of adult plant leaf rust resistance genes Lr48 and Lr49 in common wheat. Theor Appl Genet 117:307–312

    Article  CAS  PubMed  Google Scholar 

  • Bassam BJ, Caetano-Anolles G, Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196:80–83

    Article  CAS  PubMed  Google Scholar 

  • Brodny U, Nelson RR, Gregory LV (1985) The residual and interactive expressions of “defeated” wheat stem rust resistance genes. Phytopathology 76:546–549

    Article  Google Scholar 

  • Bryan GJ, Collins AJ, Stephenson P, Orry A, Smith JB, Gale MD (1997) Isolation and characterization of microsatellites from hexaploid bread wheat. Theor Appl Genet 94:557–563

    Article  CAS  Google Scholar 

  • Chen WQ, Qin QM, Chen YL, Wu ZH, Ma ZQ, Liao Q (2001) Leaf rust resistance of 40 wheat cultivars in China. Acta Phytopathol Sin 31:16–25

    Google Scholar 

  • Chen XC, Li X, Li ZF, Zhang H, Chen H, Gao M, Liu DQ (2010) Molecular mapping of leaf rust resistance gene LrG98 in Chinese wheat line Guizhou98-18. Acta Phytopathol Sin 40:489–494

    Google Scholar 

  • Dyck PL (1991) Genetics of adult plant leaf rust resistance in ‘Chinese Spring’ and ‘Sturdy’ wheats. Crop Sci 24:309–311

    Article  Google Scholar 

  • Dyck PL, Samborski DJ (1968) Host-parasite interactions involving two genes for leaf rust resistance in wheat. In: Proceedings of 3rd International Wheat Genetics Symposium Australian Academy of Science, Canberra, pp 245–250

  • Dyck PL, Samborski DJ, Anderson RG (1966) Inheritance of adult plant leaf rust resistance derived from the common wheat varieties Exchange and Frontana. Can J Genet Cytol 8:665–671

    Article  Google Scholar 

  • Guyomarc’h H, Sourdille P, Charmet G, Edwards KJ, Bernard M (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat. Theor Appl Genet 104:1164–1172

    Article  PubMed  Google Scholar 

  • Haile D, Nigussie-Dechassa R, Abdo W, Girma F (2013) Seeding rate and genotype effects on agronomic performance and grain protein content of durum wheat (Triticum turgidum L. var. durum) in south-eastern Ethiopia. Afr J Food Agric Nutr Dev 13:7693–7710

    CAS  Google Scholar 

  • Kerber ER, Dyck PL (1990) Transfer to hexaploid wheat of linked genes for adult-plant leaf rust and seedling stem rust resistance from an amphiploid of Aegilops speltoides × Triticum monococcum. Genome 33:530–537

    Article  CAS  Google Scholar 

  • Knott DR (1989) The wheat-rust breeding for resistance. In: Frankel R, Grossman M, Linskens F, Maliga P, Riley R (eds) Monographs on theoretical and applied genetics, vol 12. Springer, Berlin

    Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Article  Google Scholar 

  • Li X, Li ZF, Li YN, Zhao ZQ, Liu DQ, Wang CF, Gao LJ, Sun DJ (2010a) Genetic analysis and molecular mapping of leaf rust resistance gene in wheat line Xinong 1163-4. Sci Agric Sin 43:2397–2402

    CAS  Google Scholar 

  • Li ZF, Xia XC, He ZH, Li X, Zhang LJ, Wang HY, Meng QF, Yang WX, Li GQ, Liu DQ (2010b) Seedling and slow rusting resistance to leaf rust in Chinese wheat cultivars. Plant Dis 94:45–53

    Article  CAS  Google Scholar 

  • Long DL, Kolmer JA (1989) A North American system of nomenclature for Puccinia recondita f.sp. tritici. Phytopathology 79:525–529

    Article  Google Scholar 

  • Manly FF, Cudmore RH, Meer JM (2001) Map manager QTX, cross platform software for genetic mapping. Mamm Genome 12:930–932

    Article  CAS  PubMed  Google Scholar 

  • McIntosh RA, Dyck PL (1975) Cytogenetical studies in wheat VII. Gene Lr23 for reaction to Puccinia recondita in Gabo and related cultivars. Aust J Biol Sci 28:201–211

    Google Scholar 

  • McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts: an atlas of resistance genes. CSIRO Publishing, Melbourne

    Book  Google Scholar 

  • McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2014) Catalogue of gene symbols for wheat: 2013–2014 supplement. http://www.shigen.nig.ac.jp/wheat/komugi/genes/macgene/supplement2013.pdf

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Park RF, Mohler V, Nazari K, Singh D (2014) Characterisation and mapping of gene Lr73 conferring seedling resistance to Puccinia triticina in common wheat. Theor Appl Genet 127:2041–2049

    Article  CAS  PubMed  Google Scholar 

  • Pretorius ZA, Wilcoxson RD, Long DL, Schafer JF (1984) Detecting wheat leaf rust resistance gene Lr13 in seedings. Plant Dis 68:585–586

    Article  Google Scholar 

  • Röder MS, Plaschke J, Konig SU, Borner A, Sorrells ME, Tanksley SD, Ganal MW (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Mol Gen Genet 246:327–333

    Article  PubMed  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed Central  PubMed  Google Scholar 

  • Roelfs AP, Singh RP, Saari EE (1992) Rust diseases of wheat: concepts and methods of disease management. CIMMYT, Mexico

    Google Scholar 

  • Senior ML, Heun M (1993) Mapping maize microsatellites and polymerase chain reaction confirmation of the targeted repeats using a CT primer. Genome 36:884–889

    Article  CAS  PubMed  Google Scholar 

  • Seyfarth R, Feuillet C, Schachermayr G, Winzeler M, Keller B (1999) Development of a molecular marker for the adult plant leaf rust resistance gene Lr35 in wheat. Theor Appl Genet 99:554–560

    Article  CAS  PubMed  Google Scholar 

  • Seyfarth R, Feuillet C, Schachermayr G, Mesmer M, Winzeler M, Keller B (2000) Molecular mapping of the adult-plant leaf rust resistance gene Lr13 in wheat (Triticum aestivum L.). J Genet Breed 54:193–198

    CAS  Google Scholar 

  • Sharp PJ, Kreis M, Shewry PR, Gale MD (1988) Location of β-amylase sequence in wheat and its relatives. Theor Appl Genet 75:286–290

    Article  CAS  Google Scholar 

  • Singh RP, Huerta-Espino J, Rajaram S (2000) Achieving near-immunity to leaf and stripe rusts in wheat by combining slow rusting resistance genes. Acta Phytopathol Hung 35:133–139

    CAS  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  CAS  PubMed  Google Scholar 

  • Sourdille P, Guyomarc’h H, Baron C, Gandon B, Chiquet V, Artiguenave F, Edwards K, Foisset N, Dufour P (2001) Improvement of the genetic maps of wheat using new microsatellite markers. Plant Anim Genome IX Abstracts, p 167

  • Sourdille P, Singh S, Cadalen T, Brown-Guedira GL, Gay G, Qi L, Gill BS, Dufour P, Murigneux A, Bernard M (2004) Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.). Funct Integr Genom 4:12–25

    Article  CAS  Google Scholar 

  • Xing LF, Wang CF, Xia XC, He ZH, Chen WQ, Liu TG, Li ZF, Liu DQ (2014) Molecular mapping of leaf rust resistance gene LrFun in Romanian wheat line Fundulea 900. Mol Breeding 33:931–937

    Article  CAS  Google Scholar 

  • Zhang H, Xia XC, He ZH, Li X, Li ZF, Liu DQ (2011) Molecular mapping of leaf rust resistance gene LrBi16 in Chinese wheat cultivar Bimai 16. Mol Breeding 28:527–534

    Article  CAS  Google Scholar 

  • Zhao XL, Zheng TC, Xia XC, He ZH, Liu DQ, Yang WX, Yin GH, Li ZF (2008) Molecular mapping of leaf rust resistance gene LrZH84 in Chinese wheat line Zhou 8425B. Theor Appl Genet 117:1069–1075

    Article  CAS  PubMed  Google Scholar 

  • Zhou HX, Xia XC, He ZH, Li X, Wang CF, Li ZF, Liu DQ (2013a) Molecular mapping of leaf rust resistance gene LrNJ97 in Chinese wheat line Neijiang 977671. Theor Appl Genet 126:2141–2147

    Article  CAS  PubMed  Google Scholar 

  • Zhou Y, Xia XC, He ZH, Li X, Li ZF, Liu DQ (2013b) Fine mapping of leaf rust resistance gene LrZH84 using expressed sequence tag and sequence-tagged site markers, and allelism with other genes on wheat chromosome 1B. Phytopathology 103:169–174

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We are grateful to the critical review of this manuscript by Prof. R.A. McIntosh, Plant Breeding Institute, University of Sydney, Australia. This project was supported by National Natural Science Foundation of China (31361140367 and 31571662), Natural Science Foundation of Hebei Province (C2014204113) and Doctoral Fund of Baoding College (2014Z4).

Author information

Author notes

    Authors and Affiliations

    1. College of Plant Protection, Biological Control Center for Plant Diseases and Plant Pests of Hebei, Hebei Agricultural University, 289 Lingyusi Street, Baoding, 071001, Hebei, China

      Cuifen Wang, Peipei Zhang, Zhanjun Yao, Jinyan Qin, Zaifeng Li & Daqun Liu

    2. Zhoukou Academy of Agricultural Sciences, Jianshe East Road, Zhoukou, 466001, Henan, China

      Guihong Yin

    3. Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China

      Xianchun Xia & Zhonghu He

    4. International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South Street, Beijing, 100081, China

      Zhonghu He

    Authors
    1. Cuifen Wang
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Guihong Yin
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Xianchun Xia
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. Zhonghu He
      View author publications

      You can also search for this author in PubMed Google Scholar

    5. Peipei Zhang
      View author publications

      You can also search for this author in PubMed Google Scholar

    6. Zhanjun Yao
      View author publications

      You can also search for this author in PubMed Google Scholar

    7. Jinyan Qin
      View author publications

      You can also search for this author in PubMed Google Scholar

    8. Zaifeng Li
      View author publications

      You can also search for this author in PubMed Google Scholar

    9. Daqun Liu
      View author publications

      You can also search for this author in PubMed Google Scholar

    Corresponding authors

    Correspondence to Zaifeng Li or Daqun Liu.

    Ethics declarations

    Conflict of interest

    The authors declare that there are no conflicts of interest in the reported research.

    Additional information

    Cuifen Wang and Guihong Yin have contributed equally to this work.

    Electronic supplementary material

    Below is the link to the electronic supplementary material.

    Supplementary material 1 (DOC 76 kb)

    Supplementary material 2 (DOC 78 kb)

    Rights and permissions

    Reprints and permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Wang, C., Yin, G., Xia, X. et al. Molecular mapping of a new temperature-sensitive gene LrZH22 for leaf rust resistance in Chinese wheat cultivar Zhoumai 22. Mol Breeding 36, 18 (2016). https://doi.org/10.1007/s11032-016-0437-3

    Download citation

    • Received:

    • Accepted:

    • Published:

    • DOI: https://doi.org/10.1007/s11032-016-0437-3

    Keywords

    Search

    Navigation