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Identification of quantitative trait loci for bruchid (Caryedon serratus Olivier) resistance components in cultivated groundnut (Arachis hypogaea L.)

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Abstract

Groundnut bruchid (Caryedon serratus Olivier) is a major storage insect pest that significantly lowers the quality and market acceptance of the produce. Screening for resistance against groundnut bruchid in field conditions is difficult due to the variation in environmental factors and possible occurrence of biotypes. Hence, identification of tightly linked markers or quantitative trait loci (QTLs) is needed for selection and pyramiding of resistance genes for durable resistance. A population of recombinant inbred lines derived from a cross between VG 9514 (resistant) and TAG 24 (susceptible) was screened for five component traits of bruchid resistance in 2 years. The same population was genotyped with 221 polymorphic marker loci. A genetic linkage map covering 1,796.7 cM map distance was constructed with 190 marker loci in cultivated groundnut. QTL analysis detected thirteen main QTLs for four components of bruchid resistance in nine linkage groups and 31 epistatic QTLs for total developmental period (TDP). Screening in 2 years for bruchid resistance identified two common main QTLs. The common QTL for TDP, qTDP-b08, explained 57–82 % of phenotypic variation, while the other common QTL for adult emergence, qAE2010/11-a02, explained 13–21 % of phenotypic variation. Additionally, three QTLs for TDP, adult emergence and number of holes and one QTL for pod weight loss were identified which explained 14–39 % of phenotypic variation. This is the first report on identification of multiple main and epistatic loci for bruchid resistance in groundnut.

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Acknowledgments

The authors are grateful to the Head, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre for encouragement and critical comments. The technical help from R. K. Sachan, T. Chalapathi and Sujit Tota during the field experiments is duly acknowledged.

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  1. Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, 400085, India

    Suvendu Mondal, Ashok B. Hadapad, Poonam A. Hande & Anand M. Badigannavar

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  1. Suvendu Mondal
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  2. Ashok B. Hadapad
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  3. Poonam A. Hande
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Correspondence to Suvendu Mondal.

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11032_2013_11_MOESM1_ESM.xls

Table S1: Details of polymorphic marker loci used to construct the genetic linkage map in cultivated groundnut in this study. (XLS 85 kb)

11032_2013_11_MOESM2_ESM.xls

Table S2: Details of epistatic (additive x additive) QTLs for total developmental period (TDP) of groundnut bruchid resistance. (XLS 27 kb)

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Mondal, S., Hadapad, A.B., Hande, P.A. et al. Identification of quantitative trait loci for bruchid (Caryedon serratus Olivier) resistance components in cultivated groundnut (Arachis hypogaea L.). Mol Breeding 33, 961–973 (2014). https://doi.org/10.1007/s11032-013-0011-1

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