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Antioxidant Synergetic Effect Between the Peptides Derived from the Egg White Pentapeptide Trp-Asn-Trp-Ala-Asp

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Abstract

When foods are intaken together, the antioxidant activity of mixtures may present synergistic, additive, or antagonistic interactions. The aim of this study was to investigate the oxygen radical absorbance capacity (ORAC) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonicacid) diammonium salt (ABTS) radical scavenging activity of (Trp-Asn-Trp-Ala-Asp) WNWAD, a pentapeptide derived from egg white ovomucin pepsin hydrolysates, and its fragments as well as the effect of WNWAD on cellular antioxidant enzymes activities. The ORAC assays demonstrated that combined group (WN + WAD) significantly improved the antioxidant activities, while combined group (WNW + AD) decreased the antioxidant activities. The ABTS assays showed peptide fragments (WNW, WN, WAD) and its mixture (WNW + AD, WN + WAD) have lower EC50 than antioxidant peptide WNWAD in ABTS assay. Various combinations of peptide were found to have synergistic interaction, and synergistic interaction exhibited certain dose-dependently interactions. At cellular level, the activities of SOD, GSH-Px, CAT were higher while the level of MDA, LDH release were lower in the peroxide + peptide-treated group when compared to the peroxide exposed group. Results in this study imply the importance of exploiting synergism interactions in the development of new functional food.

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Abbreviations

WNWAD:

Trp-Asn-Trp-Ala-Asp

WNW:

Trp-Asn-Trp

WN:

Trp-Asn

WAD:

Trp-Ala-Asp

AD:

Ala-Asp

ORAC:

Oxygen radical absorbance capacity

DMEM:

Dulbeco’s Modified Eagle’s Medium

PBS:

Phosphate buffer saline

ABTS:

2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulphonicacid) diammonium salt

Hep-G2:

Human hepatocellular carcinoma cells

MDA:

Malonic dialdehyde

CAT:

Catalase

SOD:

Superoxide dismutase

LDH:

Lactate dehydrogenase

GSH-Px:

Glutathione peroxidase

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Acknowledgements

This work was supported by Specialized Research Fund for the Doctoral Program of Higher Education (No. 20130061110088) and the National Natural Science Foundation of China (No. 31601486 and 31471597).

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Authors and Affiliations

  1. Laboratory of Nutrition and Functional Food, Jilin University, Changchun, 130062, People’s Republic of China

    Deju Zhang, Yizhen Wang, Menglei Xu, Long Ding, Ting Zhang & Jingbo Liu

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  1. Deju Zhang
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  2. Yizhen Wang
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  3. Menglei Xu
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  4. Long Ding
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  5. Ting Zhang
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  6. Jingbo Liu
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Correspondence to Jingbo Liu.

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Zhang, D., Wang, Y., Xu, M. et al. Antioxidant Synergetic Effect Between the Peptides Derived from the Egg White Pentapeptide Trp-Asn-Trp-Ala-Asp. Int J Pept Res Ther 23, 509–518 (2017). https://doi.org/10.1007/s10989-017-9585-5

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