Introduction

Honey has been used to treat a variety of ailments through topical application, but only recently have the antiseptic and antioxidant properties of honey been reported (Masalha et al. 2018). Honey is a mixture of sugars, proteins, polyphenols, vitamins, minerals and free amino acids. The antioxidant activity of honey is an important aspect correlating with its anti-inflammatory and wound healing actions (Jerkovic et al. 2015; Masalha et al. 2018). Honey has been found to have antioxidant activity, measured as the ability to remove free radicals involved in infection-induced inflammation (Almasaudi et al. 2017; Kumul et al. 2015) and to reduce the damage caused to the colon in colitis in rats (Nooh and Nour-Eldien 2016; Teobaldi et al. 2018).

Proteins make up about 0.2 to 0.5% of honey, but the biological activities of honey proteins are yet largely unknown. Previous studies showed that honey proteins are mainly the major royal jelly proteins MRJP (Chua et al. 2015). The MRJP family is composed of nine members, MRJP 1 to 9, sharing primary structures but differ in their molecular weights (Ramanathan et al. 2018). A MALDI-TOF-MS study identified MRJP1 as the most abundant protein in honey bee product (Won et al. 2008). In a recent proteomic study of honey, five major proteins were identified in any type of honey, belonging to the family of MRJP 1–5 (Di Girolamo et al. 2012). Structural characterization of honey proteins have recently been explored (Chua et al. 2013; Chua et al. 2015), but their contribution in the biological function of honey remains obscure.

Although some of the antioxidant activity of honey has been contributed from phenolic compounds (Brudzynski and Maldonado-Alvarez 2018; Masalha et al. 2018), studies exploring the antioxidant activity of honey proteins and peptides are lacking. Thus, it is important to identify the bioactive peptides of honey proteins, which may be responsible for the antioxidant activity of honey. This study is aimed to explore the antioxidant bio-peptides in the honey proteins through gastrointestinal digestion simulation. Because many diseases are associated with the generation of superoxide radicals, we employed xanthine-xanthine oxidase-system that generates superoxide radicals to explore the superoxide scavenging potential of honey proteins and peptides. We also took advantage of the redox active human colonic epithelial cells (HCT-116) and yeast cells (YNN27 Saccharomyces cerevisiae) to evaluate attenuation of the intracellular oxidative stress by honey proteins and peptides.

Materials and methods

Materials

Pure Renge honey was purchased from Takano Apiary (Kagoshima, Japan). Xanthine (X), xanthine oxidase (XOD), nitroblue tetrazolium (NBT), 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), 2,2-Diphenyl- 1-picrylhydrazyl (DPPH), pepsin, trypsin andα-chymotrypsin were from Sigma-Aldrich (Tokyo, Japan). Diethyl maleate (DEM) and hydrogen peroxide (H2O2) were from Nacalai tesque (Tokyo, Japan). Alpha-cyano-4-hydroxy-cinnamic acid (α-HCCA) was from Bruker Daltonik (Bremen, Germany). McCoy 5a medium was from ICN (Invitrogen, Japan). Sephacryl S-100 was product of Amersham-Pharmacia Biotech (Tokyo, Japan).

Gastrointestinal simulated digestion of crude proteins

Sugar, vitamins, minerals, polyphenols and free amino acids were removed from raw honey by dialysis against dH2O using 1000 MWCO tubes as described earlier (Chua et al. 2015). The retentate was lyophilized and referred to as crude total honey proteins (THP). The proximate protein content of THP was 94%, as assessed by Bradford proteins assay (Bio-Rad, Japan) using bovine serum albumin as standard. The THP in HCl solution (pH 2.0 or 4.0) was mixed with pepsin to give pepsin-to-protein weight ratio of 1:30. Upon incubation for 2 h at 37 °C, reactions were heated at 85 °C for 5 min to inactivate pepsin and centrifuged at 3000×g for 10 min. The supernatants were adjusted to pH 7.0 then lyophilized, referred to as pepsin digested-HP at pH 2 (HP-p2) and pepsin digested-HP at pH 4 (HP-p4). Digests were dissolved in 50 mmol/L Na-phosphate buffer (pH 7.5) then trypsin or α-chymotrypsin was added individually or together at final enzyme-to-substrate weight ratio of 1/100. After incubation for 2 h at 37 °C reactions were heat inactivated as above and freeze dried. Reactions predigested with pepsin at pH 2.0 were referred to as HPp2-T, HPp2-α and HPp2-α/T when digested with trypsin, α-chymotrypsin and both enzymes, respectively. Reactions predigested with pepsin at pH 4.0 were referred to as HPp4-T, HPp4-α and HPp4-α/T when digested with trypsin, α-chymotrypsin and both enzymes, respectively.

Isolation of the active peptides

The most active hydrolysate was fractionated using Sephacryl S-100 column, eluted with 12.5 mmol/L pyridine-acetate buffer (pH 5.5). Peptide fractions were collected, vacuum dried and re-suspended in Milli-Q water. The profile and degree of protein hydrolysis were evaluated by SDS-PAGE under reducing condition on 4–15% acrylamide gels, according to standard protocols (Laemmli 1970). Protein bands were visualized with Coomassie Brilliant Blue R-250 (CBB). Gel was detained with methanol:acetic acid: water (2:1:7 vol/vol) solution.

Antioxidant activity

The antioxidant activity was assessed by two methods: (1) superoxide radical-scavenging assay, and (2) DPPH reduction assay. The superoxide generated by xanthine/xanthine oxidase (X/XOD) system in the presence of NBT as a probe was employed to assess superoxide-scavenging activity (Ibrahim et al. 2018). The scavenging capacity is expressed as the degree of NBT reduction by superoxide and absorbance measured at 562 nm. A reaction mixture (100 μL) containing 41.4 μmol/L NBT, 5 mU xanthine and protein or peptide samples (100 μg/mL) in phosphate buffer (pH 8.0) in a 96-well plate was mixed with 100 μL of 5 mU XOD. Control (Ctrl) contained water instead of protein sample. The flux of superoxide anion was monitored at 562 nm (37 °C) kinetically for 20 min using Ultrospec Biotrak II microplate reader (Amersham Biosciences, Uppsala, Sweden). The results were expressed as the rate of absorbance change, by subtracting the reading at 0 time from the subsequent readings; (∂Abs) =Abs (given time) − Abs (0 time). Results are representative of two independent experiments in three wells per sample.

DPPH reduction was assessed by using the TLC-dot assay, as described earlier (Ahmed et al. 2015). Briefly, protein samples were spotted on a thin layer chromatography (TLC) sheet. In control reaction (Ctrl) water was spotted instead of protein. The sheets were air-dried and sprayed with DPPH solution (2 mmol/L). The sheets were photographed after 15 min incubation in a dark box. Spots containing radical-scavenger will appear as white spot in a purple background. Control spot without scavenger (buffer only) will remain purple as shown in Fig. 3a top panel (Ctrl).

Human epithelial cells model for oxidative stress

The ability of antioxidant peptides to protect eukaryotic cells against oxidative stress was assessed by inducing oxidative stress with H2O2 or DEM (highly oxidative stress inducer). For human epithelial cells (HCT-116), cells were suspended in McCoy’s 5a medium to 105 cells/mL in 96-well black plate. A 100 μL containing peptide (200 μg/mL) and an oxidizing agent (1 mmol/L H2O2 or 2 mmol/L of DEM) was added to 100 μL cells suspension and incubated for 1 h at 37 °C under 5% CO2. Cells were pelleted at 2000×g for 5 min and fluorescent redox probe DCFH-DA (1 mmol/L) in 100 μL PBS buffer (pH 7.2) was added then incubated for 30 min at 37 °C. Emission was measured at 538 nm upon excitation at 485 nm using Infinite M200 FA fluorescence microplate reader (Tecan, Japan). Data are presented as mean ± standard deviation as percentage of viability. The rest of cells were used for fluorescence microscopic observation. Cells were washed two times with cold PBS and fixed with 2% paraformaldehyde in PBS, to preserve the chemical and physical characteristics of cells. Cell fluorescence was observed with Keyence BZ-9000 fluorescence microscope (Eurotek Inc., NJ, USA), under antifade reagent. A portion of treated cells was used to measure viability with trypan blue using TC10 automated cell counter (Bio-Rad).

Yeast cells model for oxidative stress

Saccharomyces cerevisiae (YNN27) cells in yeast extract peptone dextrose (YPD) broth at 104 CFU/mL were incubated with peptides (200 μg/mL) at 28 °C for 1 h. Hydrogen peroxide (H2O2) was added (2 mmol/L), and the incubation was extended for 48 h. A 10-μL portion of serially diluted cultures in 2 mmol/L H2O2 was spotted on YPD agar plates containing 2 mmol/L H2O2. Plates were incubated for 72 h at 28 °C and the colony forming units of yeast was estimated. Data are presented as mean ± standard deviation as percentage of survival.

MALDI-TOF-MS analysis

Peptide solutions were spotted on a polished steel target plate, then equal volume of MALDI matrix (10 mg/mL of α-HCCA in 50% acetonitrile-2.5% trifluoroacetic acid) was added and allowed to air-dry. MALDI-TOF/MS analyses were performed with Autoflex Speed mass spectrometer (Bruker Daltonik GmbH, Germany), in positive reflector mode in the mass range of 1000~3200 Da. Calibration was done by using peptides calibration standard I, and data analysis were accomplished with flexAnalysis tools (Bruker Daltonik). The sequences of peptides were identified by subjecting the major precursor ions in each peak to MS/MS analysis, using automated application to MASCOT and SEQUEST database.

Data obtained in the study were analyzed statistically using ANOVA by Excel’s data analysis tools.

Results

Digestion of honey proteins

The total honey proteins (THP) were isolated through removal of sugars, amino acids, minerals and vitamins by dialysis. THP was hydrolyzed with pepsin under two pH conditions (pH 2.0 and 4.0) followed by trypsin or α-chymotrypsin or both enzymes. Proteins and hydrolysates were analyzed by SDS-PAGE under reducing condition (Fig. 1), intact THP (THP, none) exhibited 3 major protein bands at 68.5, 63.2, 50.7 kDa (bands 2, 3 and 4, respectively) and a less dense band at 74.1 kDa (band 1). Digestion of THP with trypsin (T), α-chymotrypsin (α) or both proteases (T/α) resulted in partial digestion of protein bands with the appearance of low molecular weight bands. Pepsin at pH 2.0 (HP-p2, none) produced low mass peptides with smearing at the low zone of the gel. Digestion of HP-p2 with trypsin (T) or α-chymotrypsin (α) or both proteases (T/α) converted proteins into very small peptides accompanied with a peptide band at 24 kDa with trypsin and at 17 kDa with α-chymotrypsin (α or T/α). Apparently, the band at 24 kDa is trypsin and that at 17 kDa derived from autolysis of α-chymotrypsin (Hofstee 1965; Kumar and Hein 1970). Pepsin digest at pH 4.0 (HP-p4) or its post treatment with trypsin (T) or α-chymotrypsin (α or T/α) resulted in less efficient proteolysis of proteins than that at pH 2.0. It is, thus, evident that pepsin at pH 2.0 is the most effective protease to converted THP into small peptides.

Fig. 1
figure 1

SDS-PAGE of THP subjected to gastrointestinal digestion without pepsin (THP) or with peptic digestion at pH 2.0 (HP-p2) and pH 4.0 (HP-p4) on reducing SDS 15% polyacrylamide gel. Mr., molecular weight marker; none, intact THP or pepsin alone digests; α, T/α and T, THP or peptic hydrolysates digested with α-chymotrypsin alone, α-chymotrypsin with trypsin or trypsin alone, respectively

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Antioxidant activity

The intact THP and pepsin hydrolysates (HP-p2 and HP-p4) were assessed for ability to scavenge superoxide radicals and reduce the chemical DPPH radical (Fig. 2). The THP showed remarkable decrease in the optical density (A562) of the blue diformazan, demonstrating superoxide-scavenging activity (Fig. 2a). Digestion of THP with trypsin (THP-T) or α-chymotrypsin (THP-α) or both proteases (THP-T/α) resulted in loss of superoxide scavenging activity (Fig. 2a). Pepsin hydrolysates, HP-p2 or HP-p4, exhibited superior superoxide scavenging activities (Fig. 2b and c, respectively). Treatment of HP-p2 with α-chymotrypsin (HPp2-α) showed moderate superoxide-scavenging activity (Fig. 2b). Post proteases treatment of HP-p4 resulted in loss of the scavenging activity (Fig. 2c). The ability to reduce DPPH was conducted to delineate whether the antioxidant activity is specific to oxygen radical or it is the reducing capacity of the peptides (Fig. 2, top panels). THP and its digests (T, α, T/α) showed weak DPPH-reducing capacity (Fig. 2a). However, peptic hydrolysate HP-p2 and its post protease digests (HP-p2; T, α, T/α) exhibited significant DPPH reducing capacity, as intense white spots are produced (Fig. 2b). Although HP-p4 (none) showed DPPH reducing activity, its post protease digests (T, α, T/α) resulted in almost loss of the activity (Fig. 2c). The results indicate that HP-p2 possess peptides potently able to scavenge oxygen superoxide anions and reduce the chemical radical DPPH.

Fig. 2
figure 2

Antioxidant activities of THP and its hydrolysates. The superoxide-scavenging activity was assessed at 55 μg/mL of a intact THP or b its peptic hydrolysates at pH 2.0 (HP-p2) or c pH 4.0 (HP-p4) upon digestion with trypsin (+T), α-chymotrypsin (+α) or both (+T/α). Ctrl, reaction in the absence of protein. The corresponding DPPH reduction activities are shown on the top of each panel

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Fractionation of honey peptides

The peptic hydrolysate HP-p2 was separated into 5 peptide fractions (P1 to P5) on size-exclusion column, Sephacryl S-100 column (Fig. 3a). As shown in Fig. 3a inset, fractions P3 and P4 exhibited strong DPPH reduction activities while the parent hydrolysate (HP-p2) and P5 showed moderate activities. The fast-eluting fractions (P1 and P2) showed weak activities. The HP-p2 and all fractions showed strong superoxide-scavenging activities whereas P3 and P4 showed significantly stronger scavenging activities (Fig. 3b). The results demonstrate the superiority of peptides in fractions P3 and P4 as potent antioxidants than the other peptide fractions.

Fig. 3
figure 3

Size-exclusion chromatography of the peptic hydrolysate HP-p2. Peptides elution from Sephacryl S-100 column was monitored at 280 nm (a). Superoxide-scavenging activity of the separated peptide fractions, labeled P1-P5, was kinetically measured in xanthine/XOD/NBT reduction assay performed at 37 °C for 20 min (b). Ctrl, reaction in the absence of protein. The ordinate represents the absorbance change versus time. Symbols are: Ctrl (solid circle), HP-p2 (open square), P1 (solid triangle), P2 (asterisk), P3 (open circle), P4 (solid square), P5 (solid diamond)

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Tolerance of human epithelial cells against oxidative stress

The ability of P3 and P4 to efficiently scavenge superoxide anions and reduce DPPH radical led us to anticipate that these peptides could protect eukaryotic cells against oxidative stress. The oxidative stress of human colon epithelial carcinoma cells (HCT-116) was induced by H2O2 or DEM. The non-fluorescent DCFH-DA was used to penetrate the cells, deacetylated by cellular esterase then react with ROS to metabolize into fluorescent DCF that can be detected by fluorescence spectrophotometry at 538 nm emission (Fig. 4a and b) and fluorescence microscope (Fig. 5). As shown in Fig. 4, treatment of HCT-116 cells with H2O2 (A, Mock) or DEM (B, Mock) produced high intra-cellular ROS levels. Under H2O2-induced oxidative stress, HCT-116 cells treated with THP, HP-p2, P3 or P4 exhibited lower intracellular ROS levels compared to mock-treated cells whereas the treatment with HP-p2 or P3 showed the lowest ROS levels, 28% of mock (Fig. 4a). Under DEM-induced oxidative stress, treatment of HCT-116 cells with DEM produced intra-cellular ROS levels 5 times higher than under H2O2 (Fig. 4b, Mock). ROS levels in the P3 and P4 treated groups decreased significantly (Fig. 4b). Interestingly, fluorescence microscopic observation of HCT-116 cells, oxidatively challenged by H2O2 or DEM, treated with P3 or P4 resulted in total abolishment of the DCF fluorescence (Fig. 5). The image shows a significant decrease in fluorescence in HCT-116 cells when treated with P3 or P4 that contains mainly the TSNTF peptide, almost with the same intensity as non-induced control cells, shown in supplementary materials (Fig. S1). The results demonstrate that the peptides in P3 and P4 can induce tolerance to oxidative damage of human cells.

Fig. 4
figure 4

Intracellular reactive oxygen species (ROS) of HCT-116 cells oxidatively induced with H2O2 or DEM. Cells (105 cells/mL) oxidized with H2O2 (a) or DEM (b) were pre-incubated with THP, HP-p2 or the active peptides peaks (P3 or P4) at concentration of 200 μg/mL for 1 h at 37 °C under 5% CO2. The fluorescent redox probe DCFH-DA (1 mmol/L) was added to the treated cells. After 30 min at 37 °C, cells were washed with PBS, the fluorescence emission was measured at 538 nm upon excitation at 485 nm

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Fig. 5
figure 5

Fluorescent ex vivo imaging of ROS in HCT-116 cells oxidized with H2O2 or DEM. Cells (105 cells/L) were treated as in Fig. 4 and fixed with 2% paraformaldehyde in PBS and observed with Keyence BZ-9000 fluorescence microscope under antifade reagent. The non-fluorescent DCFH-DA is oxidized by ROS into DCF, which irradiates green/yellow fluorescence into and around the stressed cells

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Survival of human epithelial and yeast cells under oxidative stress

DEM is a highly cytotoxic oxidative stress inducer with specificity to deplete intracellular glutathione (GSH). Quantitation of cells survival of the DEM-induced HCT-116 indicated severe death of mock treated cells (22% survival) while cells pretreated with P3 and P4 retained high viability, 57 and 89%, respectively (Fig. 6a). It is likely that peptides in P3 and P4 act through modulation of the cellular redox environment to a more reducing condition by direct regeneration of GSH or activation of GSH reductases. The ability of yeast cells to tolerate H2O2-induced oxidative stress was also assessed by spotting the serially diluted cells on agar plates containing 2 mM of H2O2 then determining the colony forming units (cfu) after 2 days incubation. The treated yeast cells with THP, HP-p2, P3 or P4 showed higher viabilities than the mock-treated yeast cells (Fig. 6b). However, yeast cells treated with P3 showed 100% viability compared to 57% viability of the mock-treated cells. The results indicate that peptides in P3 act as defense molecules against intracellular oxidative damage.

Fig. 6
figure 6

Viability of HCT-116 (a) and yeast (b) cells oxidized with DEM or H2O2, respectively. Cell viability of DEM-treated HCT-116 was measured using trypan blue exclusion assay (a). Viability of H2O2-treated yeast cells was measured by colony counting on YPD agar plates (b). Viability was represented as survival percentage to the non-induced cells

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Peptides identification

Peptide in P3 and P4 were analyzed by MALDI-TOF/MS. The P3 contained a major pentapeptide (TSNTF) with mass of 568.17 and two minor peptides with masses of 524.20 and 871.45 Da (Table 1 and Fig. S2). The P4 contained similar peptide patterns with the major pentapeptide (TSNTF), but lacking the 871.45 Da peptide. The overlap of peptide sequences in P3 and P4 may be due to the close proximity of peaks in Fig. 3a. All peptides were derived from the MRJP1 of honey. The major peptide (TSNTF) in P3 and P4 corresponds to the residues 208–212 of MRJP1 (Table 1 and Fig. S3). The TSNTF peptide is a conserved sequence not only in MRJP1, but also in MRJP 2, MRJP 3 and MRJP 5 (Albert et al. 1999; Ramanathan et al. 2018). The sequence of the TSNTF peptide appears resistance to tryptic digestion but HP-p2-T lost superoxide scavenging activity (Fig. 2b). The peptic hydrolysate was heated at pH 2.0 to inactivate the enzyme, while heated at pH 7.0 upon trypsin digestion. Most likely heating at neutral pH resulted in extensive aggregation leading to lose of the peptide in the precipitate. The sequence TSNTF is unique that shares no similarity to any bio-active peptides, suggesting that the peptide constitute a novel class of antioxidant peptide.

Table 1 Peptides identified by MALDI-TOF/MS in the Sephacryl-S100 active peaks of HP-p2 hydrolysates
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Discussion

Honey has been used as a food and a medicine since the ancient times, particularly for its antioxidant and antimicrobial properties (Ahmed et al. 2018; Shenoy et al. 2012). Most studies so far attributed the antioxidant activity of honey to its phenolic compounds (Combarros-Fuertes et al. 2019). But information about the contribution of honey proteins and their peptides in its antioxidant activity is lacking. In this study, it was demonstrated that honey proteins (THP) showed moderate superoxide scavenging activity (Fig. 2a) and their peptic hydrolysates (HP-p2) showed superior antioxidant activity (Fig. 2b and c). The peptide fractions P3 and P4 eluted from size exclusion chromatography of HP-p2 (Fig. 3a) were able to completely scavenge superoxide generated in XOD coupling system (Fig. 3b), and greatly reduced the intracellular reactive oxygen species (ROS) in oxidatively stressed cells (Figs. 4 and 5). When HCT-116 cells were oxidatively stressed with DEM, P4 not only reduced the intracellular ROS species, but also was able to potently rescue the viability from 22% (mock) to 89% (Fig. 6a). We have also evaluated the viability of yeast cells and it was found that P3 and P4 rescue 100 and 86% yeast cells, respectively, when oxidatively-induced with peroxide (Fig. 6b).

Diethyl maleate (DEM) is known to severely induce oxidative stress in eukaryotic cells by depleting intracellular glutathione (Geter et al. 2012). This suggests that the ability of the peptide (mainly TSNTF peptide) to enhance tolerance against oxidative stress in human cells would be attributed to the modulation of antioxidant response. The structure and amino acid sequence of a peptide dictate the antioxidant action. The TSNTF peptide is neutrally charged with 20% hydrophobicity (Fig. S3), whereas it contains three hydroxylated residues, two threonines and one serine, in addition to the phenolic phenylalanine and carboxylic aspartic acid. These amino acids are reported to play a critical role in radical scavenging because of the nucleophilic nature of their side chains (Zou et al. 2016). Particularly, the side chain of serine contains a primary hydroxyl group while in threonine is a secondary hydroxyl group which are preferentially proton transferring groups (Li et al. 2018). Serine and threonine residues in peptides have also been shown to directly interact with free radicals (Agrawal et al. 2019). Serine and threonine residues were also found able to form hydrogen bonds with DPPH radical and possess cyto-protective function by elevating the intracellular antioxidant factors such nuclear factor erythroid 2–related factor, Nrf2, and nitric oxide, NO (Maralani et al. 2012).

In mice, serine supplementation attenuated the effects of oxidative stress induced by treatment with diquat, a bipyridyl herbicide which generates ROS, by supporting GSH synthesis (Zhou et al. 2017). In the same experiment, mouse hepatocytes were cultured with diquat and Ser, Ser effects on oxidative stress are mediated by its promotion of cystathionine β-synthase (CBS) activity which is utilized to synthesize GSH. This function gives Ser a role in promoting the entry of homocysteine into the transsulfuration pathway to enhance GSH production Additionally, in mice, Ser supplementation helped reduce oxidative stress and steatosis induced by feeding a high-fat diet through epigenetically modulating the abundance of genes for GSH synthesis to promote synthesis and reduce ROS (Zhou et al. 2018). In fish fed optimal amounts of threonine showed increased plasma antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) (Habte-Tsion et al. 2016). These observations solidify the link between the sequence and the ex vivo antioxidant action of honey TSNTF peptide found in this study. It argues that the peptide TSNTF, containing a Ser residue and 2 Thr residues, could interact with ROS resulting in less depletion of GSH, and likely promoting GSH synthesis as well as induction of the intracellular antioxidant response enzymes (Fernandes et al. 2007).

Conclusion

The findings of this study highlight that honey proteins have anti-oxidant activity and possess a potent antioxidant pentapeptide, TSNTF. The TSNTF peptide was the major peptide in the most potent antioxidant farctions, P3 and P4 that demonstrated to be potent scavenger of superoxide radical and DPPH radical. The protective role of TSNTF peptide fraction on cell viability and antioxidant defenses of human HCT-116 cells challenged by hydrogen peroxide and diethyl maleate was demonstrated. This study is, thus, the first to report such activity by honey MRJP1 and a derived peptide. Peptic hydrolysates of honey proteins offer great promises for their potential applications in nutraceutical industry. The ex vivo antioxidant action of TSNTF peptide, able to protect human and yeast cells against oxidative damage, suggest its excellent candidacy as new bioactive peptide and a promising therapeutic peptide against oxidative stress related diseases.