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Preparation, molecular modeling and biodistribution of 99mTc-phytochlorin complex

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Abstract

Phytochlorin [21H, 23H-Porphine-7-propanoicacid, 3-carboxy-5-(carboxymethyl)13-ethenyl-18-ethyl-7,8-dihydro-2,8,12,17-tetramethyl-,(7S,8S)] was labeled with 99mTc and the factors affecting the labeling yield of 99mTc-phytochlorin complex were studied in details. At pH 10, 99mTc-phytochlorin complex was obtained with a high radiochemical yield of 98.4 ± 0.6 % by adding 99mTc to 100 mg phytochlorin in the presence of 75 μg SnCl2·2H2O after 30 min reaction time. The molecular modeling study showed that the structure of 99mTc-phytochlorin complex presents nearly linear HO–Tc–OH unit with an angle of 179.27° and a coplanar Tc(N1N2N3N4) unit. Biodistribution of 99mTc-phytochlorin complex in tumor bearing mice showed high T/NT ratio (T/NT = 3.65 at 90 min post injection). This preclinical study showed that 99mTc-phytochlorin complex is a potential selective radiotracer for solid tumor imaging and afford it as a new radiopharmaceutical suitable to proceed through the clinical trials for tumor imaging.

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Authors and Affiliations

  1. Labeled Compounds Department, Hot Lab. Center, Atomic Energy Authority, P.O. Box 13795, Cairo, Egypt

    Mohamed A. Motaleb

  2. Chemistry Department, Faculty of Science, Benha University, Benha, 13518, Egypt

    Mostafa Y. Nassar

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  1. Mohamed A. Motaleb
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  2. Mostafa Y. Nassar
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Correspondence to Mohamed A. Motaleb.

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Motaleb, M.A., Nassar, M.Y. Preparation, molecular modeling and biodistribution of 99mTc-phytochlorin complex. J Radioanal Nucl Chem 299, 1759–1766 (2014). https://doi.org/10.1007/s10967-014-2920-9

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  • DOI: https://doi.org/10.1007/s10967-014-2920-9

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