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Immunological properties of 60Co gamma-rays irradiated bothropstoxin-I

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Abstract

We investigated the immunological behavior of BTHX-1, before and after irradiation. SDS-PAGE showed that BTHX-1 irradiated in the presence of NaNO3, had its structure preserved. Animals’ plasma immunized with native BTHX-1 had high IgG1 titers. The irradiated protein induced high titers of IgG2b. When the toxin was irradiated with t-butanol, there was a slight decrease in the production of IgG2b. Real-time PCR showed that both the IL-2 as for IL4 was more expression from the cells of the animals immunized with BTHX-1 irradiated. These results indicate that irradiation of proteins leads to significant structural modifications.

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Authors and Affiliations

  1. Instituto de Pesquisas Energéticas e Nucleares (IPEN/CNEN-SP), Av. Professor Lineu Prestes 2242, 05508-000, São Paulo, SP, Brasil

    J. A. Baptista, D. P. Vieira, A. J. Galisteo-Júnior, P. Caproni, M. Casare, H. F. de Andrade-Júnior, P. J. Spencer & N. Nascimento

  2. Instituto de Medicina Tropical de Sao Paulo — Lab. de Protozoologia, São Paulo, SP, Brazil

    D. P. Vieira, A. J. Galisteo-Júnior & H. F. de Andrade-Júnior

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  1. J. A. Baptista
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  2. D. P. Vieira
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  3. A. J. Galisteo-Júnior
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  4. P. Caproni
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  5. M. Casare
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  6. H. F. de Andrade-Júnior
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  7. P. J. Spencer
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  8. N. Nascimento
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Correspondence to J. A. Baptista.

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Baptista, J.A., Vieira, D.P., Galisteo-Júnior, A.J. et al. Immunological properties of 60Co gamma-rays irradiated bothropstoxin-I. J Radioanal Nucl Chem 279, 817–821 (2009). https://doi.org/10.1007/s10967-008-7416-z

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  • DOI: https://doi.org/10.1007/s10967-008-7416-z

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