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Cloning, Expression, and Characterization of Siamese Crocodile (Crocodylus siamensis) Hemoglobin from Escherichia coli and Pichia pastoris

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Abstract

Recombinant Crocodylus siamensis hemoglobin (cHb) has been constructed and expressed using Escherichia coli as the expression system in conjunction with a trigger factor from the Cold-shock system as the fusion protein. While successful processing as soluble protein in E. coli was achieved, the net yields of active protein from downstream purification processes remained still unsatisfactory. In this study, cHb was constructed and expressed in the eukaryotic expression system Pichia pastoris. The results showed that cHb was excreted from P. pastoris as a soluble protein after 72 h at 25 °C. The amino acid sequence of recombinant cHb was confirmed using LC–MS/MS. Indeed, the characteristic of Hb was investigated by external heme incorporation. The UV–Vis profile showed a specific pattern of the absorption at 415 nm, indicating the recombinant cHb was formed complex with heme, resulting in active oxyhemoglobin (OxyHb). This result suggests that the heme molecules were fully combined with heme binding site of the recombinant cHb, thus producing characteristic red color for the OxyHb at 540 and 580 nm. The results revealed that the recombinant cHb was prosperously produced in P. pastoris and exhibited a property as protein–ligand binding. Thus, our work described herein offers a great potential to be applied for further studies of heme-containing protein expression. It represents further pleasing option for protein production and purification on a large scale, which is important for determination and characterization of the authenticity features of cHb proteins.

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Abbreviations

AOX1:

Alcohol oxidase 1 promoter

cHb:

Crocodylus siamensis hemoglobin

Hb:

Hemoglobin

IBs:

Inclusion body

IMAC:

Immobilized metal affinity chromatography

IPTG:

Isopropyl β-d-1-thiogalactopyranoside

LB:

Luria–Bertani medium

ORF:

Open reading frame

OxyHb:

Oxyhemoglobin

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

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Acknowledgments

This research was financially supported by the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission, Khon Kaen University, Thailand (Grant no. NRU571004), the Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI) funded by Khon Kean University. The authors thank the Sriracha Moda Co. Ltd., Chon Buri, Thailand for providing crocodile blood samples.

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Authors and Affiliations

  1. Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Khon Kaen University, Khon Kaen, 40002, Thailand

    Preeyanan Anwised, Nisachon Jangpromma, Rina Patramanon, Sakda Daduang & Sompong Klaynongsruang

  2. Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    Preeyanan Anwised, Rina Patramanon & Sompong Klaynongsruang

  3. Office of the Dean, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    Nisachon Jangpromma

  4. Sriracha Moda Farm, Chon Buri, 20110, Thailand

    Theeranan Temsiripong

  5. Division of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand

    Sakda Daduang

  6. Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand

    Sarawut Jitrapakdee

  7. Department of Bioscience, School of Agriculture, Tokai University, Aso, Kumamoto, 869-1404, Japan

    Tomohiro Araki

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  1. Preeyanan Anwised
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Correspondence to Sompong Klaynongsruang.

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Anwised, P., Jangpromma, N., Temsiripong, T. et al. Cloning, Expression, and Characterization of Siamese Crocodile (Crocodylus siamensis) Hemoglobin from Escherichia coli and Pichia pastoris . Protein J 35, 256–268 (2016). https://doi.org/10.1007/s10930-016-9669-7

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  • DOI: https://doi.org/10.1007/s10930-016-9669-7

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