Skip to main content
SpringerLink
Log in

Characterization of a Methionine Sulfoxide Reductase B from Tomato (Solanum lycopersicum), and Its Protecting Role in Saccharomyces cerevisiae

  • Published:
The Protein Journal Aims and scope Submit manuscript

Abstract

In the present study, we isolated a methionine sulfoxide reductase B gene, termed SlMSRB1, from tomato (Solanum lycopersicum). In the organ-specific analysis, high expression levels of SlMSRB1 were detected in red mature fruits, leaves and flowers while low transcriptional levels of SlMSRB1 mRNA were observed in stems and roots. In the green fluorescence analysis of SlMSRB1- overexpressed Arabidopsis, signal corresponding to SlMSRB1 was merely detected in chloroplast, suggesting that tomato MSRB1 is a chloroplastial localization protein. Substrate specificity analysis of recombinant SlMSRB1 showed that the enzyme was only targeted to the R epimer of methionine sulfoxide (MetSO) and was able to convert both free and protein-bound MetSO back to methionine in the presence of dithithreitol (DTT). In addition, SlMSRB1 exhibited no activity in thioredoxin dependent system or the substitution of cysteine at position 181 in the DTT-dependent reduction system. Finally, overexpression of SlMSRB1 in yeast revealed that the SlMSRB1 gene might play a critical role in protecting Saccharomyces cerevisiae against oxidative stress.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Abbreviations

DTT:

Dithithreitol

HPLC:

High performance liquid chromatography

IPTG:

Isopropyl-β-d-thiogalactopyranoside

MetSO:

Methionine sulfoxide

MSR:

Methionine sulfoxide reductase

MV:

Methyl viologen

TLC:

Thin layer chromatography

Trx:

Thioredoxin

References

  1. Boschi-Muller S, Azza S, Sanglier-Cianferani S, Talfournier F, Van Dorsselear A, Branlant G (2000) J Biol Chem 275:35908–35913

    Article  CAS  Google Scholar 

  2. Boschi-Muller S, Olry A, Antoine M, Branlant G (2005) Biochim Biophys Acta 1703:231–238

    Article  CAS  Google Scholar 

  3. Bradford MM (1976) Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  4. Brot N, Weissbach L, Werth J, Weissbach H (1981) Proc Natl Acad Sci USA 78:2155–2158

    Article  CAS  Google Scholar 

  5. Cordes S, Deikman J, Margossian LJ, Fischer RL (1989) Plant Cell 1:1025–1034

    CAS  Google Scholar 

  6. Dai C, Wang MH (2012) Mol Biol Rep 39:6297–6308

    Article  CAS  Google Scholar 

  7. Ding D, Sagher D, Laugier E, Rey P, Weissbach H, Zhang XH (2007) Biochem Biophys Res Commun 361:629–633

    Article  CAS  Google Scholar 

  8. Gabbita SP, Aksenov MY, Lovell MA, Markesbery WR (1999) J Neurochem 73:1660–1666

    Article  CAS  Google Scholar 

  9. Guo X, Wu Y, Wang Y, Chen Y, Chu C (2009) Planta 230:227–238

    Article  CAS  Google Scholar 

  10. Ito H, Fukuda Y, Murata K, Kimura A (1983) J Bacteriol 153:163–168

    CAS  Google Scholar 

  11. Koc A, Gasch AP, Rutherford JC, Kim HY, Gladyshev VN (2004) Proc Natl Acad Sci USA 101:7999–8004

    Article  CAS  Google Scholar 

  12. Kwon SJ, Kwon SI, Bae MS, Cho EJ, Park OK (2007) Plant Cell Physiol 48:1713–1723

    Article  CAS  Google Scholar 

  13. Lee BC, Dikiy A, Kim HY, Gladyshev VN (2009) Biochim Biophys Acta 1790:1471–1477

    Article  CAS  Google Scholar 

  14. Lopez AP, Portales RB, López-Ráez JA, Medina-Escobar N, Blanco JM, Franco AR (2006) Physiol Plant 1266:129–139

    Article  Google Scholar 

  15. Lowther WT, Brot N, Weissbach H, Honek JF, Matthews BW (2000) Proc Natl Acad Sci USA 97:6463–6468

    Article  CAS  Google Scholar 

  16. Minetti G, Balduini C, Brovelli A (1994) Ital J Biochem 43:273–283

    CAS  Google Scholar 

  17. Moskovitz J, Berlett BS, Poston JM, Stadtman ER (1997) Proc Natl Acad Sci USA 94:9585–9589

    Article  CAS  Google Scholar 

  18. Neiers F, Kriznik A, Boschi-Muller S, Branlant G (2004) J Biol Chem 279:42462–42468

    Article  CAS  Google Scholar 

  19. Oh SK, Baek KH, Seong ES, Joung YH, Choi GJ, Park JM, Cho HS, Kim EA, Lee S, Choi D (2010) Plant Physiol 154:245–261

    Article  CAS  Google Scholar 

  20. Rodrigo MJ, Moskovitz J, Salamini F, Bartels D (2002) Mol Genet Genomics 267:613–621

    Article  CAS  Google Scholar 

  21. Romero HM, Berlett BS, Jensen PJ, Pell EJ, Tien M (2004) Plant Physiol 136:3784–3794

    Article  CAS  Google Scholar 

  22. Rouhier N, Kauffmann B, Tete-Favier F, Palladino P, Gans P, Branlant G, Jacquot JP, Boschi-Muller S (2007) J Biol Chem 282:3367–3378

    Article  CAS  Google Scholar 

  23. Rouhier N, Vieira Dos Santos C, Tarrago L, Rey P (2006) Photosynth Res 89:247–262

    Article  CAS  Google Scholar 

  24. Sadanandom A, Piffanelli P, Knott T, Robinson C, Sharpe A, Lydiate D, Murphy D, Fairbairn DJ (1996) Plant J 10:235–242

    Article  CAS  Google Scholar 

  25. Tarrago L, Laugier E, Rey P (2009) Mol Plant 2:202–217

    Article  CAS  Google Scholar 

  26. Tarrago L, Laugier E, Zaffagnini M, Marchand C, Le Marechal P, Rouhier N, Lemaire SD, Rey P (2009) J Biol Chem 284:18963–18971

    Article  CAS  Google Scholar 

  27. Tarrago L, Laugier E, Zaffagnini M, Marchand CH, Le Marechal P, Lemaire SD, Rey P (2010) J Biol Chem 285:14964–14972

    Article  CAS  Google Scholar 

  28. Vieira Dos Santos C, Cuine S, Rouhier N, Rey P (2005) Plant Physiol 138:909–922

    Article  Google Scholar 

Download references

Acknowledgments

This work was partially supported by a grant from Institute of Biosciences and Biotechnology at Kangwon National University.

Author information

Authors and Affiliations

  1. Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University, Chuncheon, Gangwon-do, 200-701, South Korea

    Changbo Dai, Likun Liu & Myeong Hyeon Wang

Authors
  1. Changbo Dai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Likun Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Myeong Hyeon Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Myeong Hyeon Wang.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PPTX 66 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dai, C., Liu, L. & Wang, M.H. Characterization of a Methionine Sulfoxide Reductase B from Tomato (Solanum lycopersicum), and Its Protecting Role in Saccharomyces cerevisiae . Protein J 32, 39–47 (2013). https://doi.org/10.1007/s10930-012-9457-y

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10930-012-9457-y

Keywords

Search

Navigation