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OsMSRA4.1 and OsMSRB1.1, two rice plastidial methionine sulfoxide reductases, are involved in abiotic stress responses

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Abstract

In proteins, methionine residues are especially sensitive to oxidation, leading to the formation of S- and R-methionine sulfoxide diastereoisomers, and these two methionine sulfoxides can be specifically reversed by two types of methionine sulfoxide reductases (MSRs), MSRA and MSRB. Previously, we have identified a gene encoding a putative MSR from NaCl-treated roots of Brazilian upland rice (Oryza sativa L. cv. IAPAR 9) via subtractive suppression hybridization (Wu et al. in Plant Sci 168:847–853, 2005). Blast database analysis indicated that at least four MSRA and three MSRB orthologs exist in rice, and two of them, OsMSRA4.1 and OsMSRB1.1, were selected for further functional analysis. Expression analysis showed that both OsMSRA4.1 and OsMSRB1.1 are constitutively expressed in all organs and can be induced by various stress conditions. Subcellular localization and in vitro activity assay revealed that both OsMSR proteins are targeted to the chloroplast and have MSR activity. Overexpression of either OsMSRA4.1 or OsMSRB1.1 in yeast enhanced cellular resistance to oxidative stress. In addition, OsMSRA4.1-overexpressing transgenic rice plants also showed enhanced viability under salt treatment. Our results provide genetic evidence of the involvement of OsMSRs in the plant stress responses.

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Abbreviations

Met:

Methionine

MetSO:

Methionine sulfoxide

MSR:

Methionine sulfoxide reductase

ROS:

Reactive oxygen species

ER:

Endoplasmic reticulum

MDA:

Malondialdehyde

GFP:

Green fluorescent protein

HPLC:

High-performance liquid chromatography

PVP:

Polyvinylpyrrolidone

TFA:

Trifluoroacetic acid

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Acknowledgments

We thank Dr. Hwa-Young Kim and Vadim N. Gladyshev (Department of Biochemistry, University of Nebraska-Lincoln) for providing the substrates of two diastereomers of methionine sulfoxide and yeast mutant strain selR/msrA, and Dr. Jackob Moskovitz (Department of Pharmacology & Toxicology, University of Kansas) for yeast strain msrA. We also thank Mr. John Wallace Moore (Institute of Molecular Plant Sciences, Edinburgh University) for the critical reading of this manuscript. This work was supported by grants from the Chinese Academy of Sciences (KSCX2-YW-N-010) and National Natural Sciences Foundation of China (30671128, 30621001, 30670195).

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Authors and Affiliations

  1. State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences (CAS), 100101, Beijing, China

    Xiaoli Guo, Yaorong Wu, Yiqin Wang, Yanmin Chen & Chengcai Chu

  2. Graduate School of the Chinese Academy of Sciences, 100039, Beijing, China

    Xiaoli Guo

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  1. Xiaoli Guo
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  2. Yaorong Wu
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  3. Yiqin Wang
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  4. Yanmin Chen
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  5. Chengcai Chu
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Correspondence to Chengcai Chu.

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X. Guo and Y. Wu contributed equally to this work.

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Guo, X., Wu, Y., Wang, Y. et al. OsMSRA4.1 and OsMSRB1.1, two rice plastidial methionine sulfoxide reductases, are involved in abiotic stress responses. Planta 230, 227–238 (2009). https://doi.org/10.1007/s00425-009-0934-2

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