Abstract
Polyphenol oxidases (PPOs) have been reported to play an important role in protecting plants from attack by herbivores. However, little is known about their role in tea. Here, we investigated the effect of PPOs on interactions between tea plants and the tea geometrid Ectropis obliqua, one of the most important insect pests of tea. Jasmonic acid (JA) treatment resulted in increases in PPO activity, and the effect of JA was dose dependent. Ectropis obliqua caterpillars grew and developed more slowly on JA-treated tea plants than on control plants, and larval weight gains depended on the JA dosage. Artificial diet complemented with PPOs reduced the growth and survival rate of E. obliqua caterpillars, and there was a negative relationship between PPO level and larval growth and survival. Unlike mechanical wounding, which is an effective inducer of tea plant PPO activity, wounding plus the herbivore regurgitant or herbivore infestation suppressed the wound-induced PPO activities, especially at 4 days after treatment. These results suggest that PPOs are an important anti-herbivore factor in tea plants, defending them against E. obliqua larvae, and that E. obliqua larvae have evolved to elude the tea plant’s defense by inhibiting the production of PPOs.
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Acknowledgments
We thank Emily Wheeler for editorial assistance. The study was sponsored by National Program on Key Basic Research Project (973 Program) (2012CB114104), National Natural Science Foundation of China (31272053; 31171862), and the Division of Science and Technology of Zhejiang Province (2011C22043).
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Zi-Wei Yang and Xiao-Na Duan contributed equally.
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Fig. S1
Mean absorbance (±SE) for the production of tea sustract, catechins, and artificial diet that catalyzed by mushroom PPO in different concentrations. Duncan’s multiple range test: capitalization letters P<0.001; lowercase P<0.05. (PDF 13 kb)
Fig. S2
Mean absorbance (±SE) for the production of catechins and artificial diet that catalyzed by tea extract in different concentrations. t-test: *P<0.05. (PDF 11 kb)
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Yang, ZW., Duan, XN., Jin, S. et al. Regurgitant Derived From the Tea Geometrid Ectropis obliqua Suppresses Wound-Induced Polyphenol Oxidases Activity in Tea Plants. J Chem Ecol 39, 744–751 (2013). https://doi.org/10.1007/s10886-013-0296-x
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DOI: https://doi.org/10.1007/s10886-013-0296-x