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Electron transport chain dysfunction by H2O2 is linked to increased reactive oxygen species production and iron mobilization by lipoperoxidation: studies using Saccharomyces cerevisiae mitochondria

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Abstract

The mitochondrial electron transport chain (ETC) contains thiol groups (−SH) which are reversibly oxidized to modulate ETC function during H2O2 overproduction. Since deleterious effects of H2O2 are not limited to –SH oxidation, due to the formation of other H2O2-derived species, some processes like lipoperoxidation could enhance the effects of H2O2 over ETC enzymes, disrupt their modulation by –SH oxidation and increase superoxide production. To verify this hypothesis, we tested the effects of H2O2 on ETC activities, superoxide production and iron mobilization in mitochondria from lipoperoxidation-resistant native yeast and lipoperoxidation-sensitized yeast. Only complex III activity from lipoperoxidation-sensitive mitochondria exhibited a higher susceptibility to H2O2 and increased superoxide production. The recovery of ETC activity by the thiol reductanct β-mercaptoethanol (BME) was also altered at complex III, and a role was attributed to lipoperoxidation, the latter being also responsible for iron release. A hypothetical model linking lipoperoxidation, increased complex III damage, superoxide production and iron release is given.

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Authors and Affiliations

  1. Facultad de Ciencias Médicas y Biológicas “Dr. Ignacio Chávez”, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Mich., México

    Christian Cortés-Rojo

  2. Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Mich., México

    Mirella Estrada-Villagómez, Mónica Clemente-Guerrero & Alfredo Saavedra-Molina

  3. Facultad de Enfermería, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Mich., México

    Elizabeth Calderón-Cortés

  4. Facultad de Estudios Superiores Iztacala, Unidad de Biomedicina, UNAM, Tlalnepantla, Edo. de México, México

    Ricardo Mejía-Zepeda

  5. School of Medicine, University of Texas Medical Branch at Galveston, Galveston, TX, USA

    Istvan Boldogh & Alfredo Saavedra-Molina

Authors
  1. Christian Cortés-Rojo
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  2. Mirella Estrada-Villagómez
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  3. Elizabeth Calderón-Cortés
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  4. Mónica Clemente-Guerrero
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  5. Ricardo Mejía-Zepeda
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  6. Istvan Boldogh
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  7. Alfredo Saavedra-Molina
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Correspondence to Alfredo Saavedra-Molina.

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Cortés-Rojo, C., Estrada-Villagómez, M., Calderón-Cortés, E. et al. Electron transport chain dysfunction by H2O2 is linked to increased reactive oxygen species production and iron mobilization by lipoperoxidation: studies using Saccharomyces cerevisiae mitochondria. J Bioenerg Biomembr 43, 135–147 (2011). https://doi.org/10.1007/s10863-011-9339-6

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  • DOI: https://doi.org/10.1007/s10863-011-9339-6

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