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Effects of ubiquinone derivatives on the mitochondrial unselective channel of Saccharomyces cerevisiae

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Abstract

Ubiquinone derivatives modulate the mammalian mitochondrial Permeability Transition Pore (PTP). Yeast mitochondria harbor a similar structure: the respiration- and ATP-induced Saccharomyces cerevisiae Mitochondrial Unselective Channel ( Sc MUC). Here we show that decylubiquinone, a well-characterized inhibitor of the PTP, suppresses Sc MUC opening in diverse strains and independently of respiratory chain modulation or redox-state. We also found that naturally occurring derivatives such as hexaprenyl and decaprenyl ubiquinones lacked effects on the Sc MUC. The PTP-inactive ubiquinone 5 (Ub5) promoted the Sc MUC-independent activation of the respiratory chain in most strains tested. In an industrial strain however, Ub5 blocked the protection elicited by dUb. The results indicate the presence of a ubiquinone-binding site in the Sc MUC.

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Abbreviations

dUb:

Decylubiquinone

dVO4 :

Decavanadate

Δψ:

Mitochondrial transmembrane potential

FCCP:

Carbonyl cyanide p-trifluoro-methoxyphenyl-hydrazone

Cyclosporine A:

CsA

PTP:

Mitochondrial permeability transition pore

Sc MUC:

Saccharomyces cerevisiae mitochondrial unselective channel

Ub5 :

Ubiquinone 5

Ub30 :

Hexaprenylquinone

Ub50 :

Decaprenylquinone

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Acknowledgments

M.G.-A. is currently supported by an American Heart Association Midwest Affiliate Postdoctoral Fellowship (13POST14060013). HLC is a CONACyT fellow enrolled in the Ms. Sc. Biochemistry program at UNAM. CUA, EGS and MRL are CONACyT fellows enrolled in the Ph. D. Biochemistry program at UNAM. Partially funded by DGAPA/PAPIIT Project IN202612. We acknowledge the technical assistance of Ramón Mendez. Mariana Valenzuela kindly helped to build the figures.

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Authors and Affiliations

  1. Dalton Cardiovascular Research Center, University of Missouri-Columbia, 134 Research Park Dr, Columbia, MO, 65211, U.S.A

    Manuel Gutiérrez-Aguilar

  2. Department of Molecular Genetics, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, UNAM, Mexico City, Mexico

    Helga M. López-Carbajal, Cristina Uribe-Alvarez, Emilio Espinoza-Simón, Mónica Rosas-Lemus, Natalia Chiquete-Félix & Salvador Uribe-Carvajal

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  1. Manuel Gutiérrez-Aguilar
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  2. Helga M. López-Carbajal
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  3. Cristina Uribe-Alvarez
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  4. Emilio Espinoza-Simón
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  5. Mónica Rosas-Lemus
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  7. Salvador Uribe-Carvajal
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Correspondence to Manuel Gutiérrez-Aguilar.

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Gutiérrez-Aguilar, M., López-Carbajal, H.M., Uribe-Alvarez, C. et al. Effects of ubiquinone derivatives on the mitochondrial unselective channel of Saccharomyces cerevisiae . J Bioenerg Biomembr 46, 519–527 (2014). https://doi.org/10.1007/s10863-014-9595-3

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  • DOI: https://doi.org/10.1007/s10863-014-9595-3

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