Abstract
The modification of human cancellous bone (hBONE) with silk fibroin/gelatin (SF/G) using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)/N-hydroxysuccini-mide (NHS) crosslinking was established. The SF/G solutions at a weight ratio of 50/50 and the solution concentrations of 1, 2, and 4 wt % were studied. SF/G sub-matrix was formed on the surface and inside pore structure of hBONE. All hBONE scaffolds modified with SF/G showed smaller pore sizes, less porosity, and slightly lower compressive modulus than unmodified hBONE. SF/G sub-matrix was gradually biodegraded in collagenase solution along 4 days. The hBONE scaffolds modified with SF/G, particularly at 2 and 4 wt % solution concentrations, promoted attachment, proliferation, and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (MSC), comparing to the original hBONE. The highest cell number, ALP activity and calcium production were observed for MSC cultured on the hBONE scaffolds modified with 4 wt % SF/G. The mineralization was also remarkably induced in the cases of modified hBONE scaffolds as observed from the deposited calcium phosphate by EDS. The modification of hBONE with SF/G was, therefore, the promising method to enhance the osteoconductive potential of human bone graft for bone tissue engineering.
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Acknowledgments
This research was supported by the Medical Association of Thailand and the Chulalongkorn University Centenary Academic Development Project. Kind supplies of human cancellous bone from Bangkok Biomaterial Center under the Patronage of H.R.H. Princess Galyani Vadhana Krom Luang Naradhiwas Rajanagarindra, Faculty of Medicine, Siriraj Hospital, and “Nangnoi Srisaket 1” cocoons from Queen Sirikit Sericulture Center, Nakhonratchasima province, Thailand, were acknowledged. We extend our thanks to Tanom Bunaprasert, M.D. for his support on the cell culture facilities at i-Tissue Laboratory, Faculty of Medicine, Chulalongkorn University.
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Vorrapakdee, R., Kanokpanont, S., Ratanavaraporn, J. et al. Modification of human cancellous bone using Thai silk fibroin and gelatin for enhanced osteoconductive potential. J Mater Sci: Mater Med 24, 735–744 (2013). https://doi.org/10.1007/s10856-012-4830-0
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DOI: https://doi.org/10.1007/s10856-012-4830-0