Abstract
Orthopaedic materials may improve its capacity to resist bacterial adherence, and subsequent infection. Our aim was to test the bacterial adherence to alpha-tocopherol (frequently named vitamin E, VE) doped or blended UHMWPE with S. aureus and S. epidermidis, compared to virgin material. Collection strains and clinical strains isolated from patients with orthopaedic infections were used, with the biofilm-developing ability as a covariable. While collection strains showed significantly less adherence to VE-UHMWPE, some clinical strains failed to confirm this effect, leading to the conclusion that VE doped or blended UHMWPE affects the adherence of some S. epidermidis and S. aureus strains, independently of the concentration in use, but the results showed important intraspecies differences and cannot be generalized.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Petty W, Spanier S, Shuster JJ, Silverthorne C. The influence of skeletal implants on incidence of infection. Experiments in a canine model. J Bone Joint Surg Am. 1985;67(8):1236–44.
Canadian Institute for Health Information. Hip and Knee Replacements in Canada—Canadian Joint Replacement Registry (CJRR) 2008–2009 Annual Report. Ottawa, Ont.: CIHI; 2009.
Darouiche RO. Treatment of infections associated with surgical implants. N Engl J Med. 2004;350(14):1422–9.
Parth M, Aust N, Lederer K. Studies on the effect of electron beam radiation on the molecular structure of ultra-high molecular weight polyethylene under the influence of alpha-tocopherol with respect to its application in medical implants. J Mater Sci Mater Med. 2002;13(10):917–21.
Wolf C, Krivec T, Blassnig J, Lederer K, Schneider W. Examination of the suitability of alpha-tocopherol as a stabilizer for ultra-high molecular weight polyethylene used for articulating surfaces in joint endoprostheses. J Mater Sci Mater Med. 2002;13(2):185–9.
Oral E, Greenbaum ES, Malhi AS, Harris WH, Muratoglu OK. Characterization of irradiated blends of alpha-tocopherol and UHMWPE. Biomaterials. 2005;26(33):6657–63.
Shibata N, Tomita N, Onmori N, Kato K, Ikeuchi K. Defect initiation at subsurface grain boundary as a precursor of delamination in ultrahigh molecular weight polyethylene. J Biomed Mater Res A. 2003;67(1):276–84.
Oral E, Wannomae KK, Hawkins N, Harris WH, Muratoglu OK. Alpha-tocopherol-doped irradiated UHMWPE for high fatigue resistance and low wear. Biomaterials. 2004;25(24):5515–22.
Oral E, Malhi AS, Muratoglu OK. Mechanisms of decrease in fatigue crack propagation resistance in irradiated and melted UHMWPE. Biomaterials. 2006;27(6):917–25.
Kinnari TJ, Esteban J, Zamora N, Fernandez R, Lopez-Santos C, Yubero F, et al. Effect of surface roughness and sterilization on bacterial adherence to ultra-high molecular weight polyethylene. Clin Microbiol Infect. 2010;16:1036–41.
Meydani SN, Han SN, Wu D. Vitamin E and immune response in the aged: molecular mechanisms and clinical implications. Immunol Rev. 2005;205:269–84.
Victor VM, Rocha M, De la Fuente M. Immune cells: free radicals and antioxidants in sepsis. Int Immunopharmacol. 2004;4(3):327–47.
Owens D, Wendt R. Estimation of surface free energy of polymers. J Appl Polymer Sci. 1969;13:1741–7.
Valle J, Toledo-Arana A, Berasain C, Ghigo JM, Amorena B, Penades JR, et al. SarA and not sigma B is essential for biofilm development by Staphylococcus aureus. Mol Microbiol. 2003;48(4):1075–87.
Esteban J, Gomez-Barrena E, Cordero J, Martin-de-Hijas NZ, Kinnari TJ, Fernandez-Roblas R. Evaluation of quantitative analysis of cultures from sonicated retrieved orthopedic implants in diagnosis of orthopedic infection. J Clin Microbiol. 2008;46(2):488–92.
Stepanovic S, Vukovic D, Hola V, Di Bonaventura G, Djukic S, Cirkovic I, et al. Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by Staphylococci. APMIS. 2007;115(8):891–9.
López-Santos M, Yubero F, Espinós J, González-Elipe A. Non-destructive depth compositional profiles by XPS peak-shape analysis. Anal Bioanal Chem. 2010;396:2757–68.
Katsikogianni M, Missirlis YF. Concise review of mechanisms of bacterial adhesion to biomaterials and of techniques used in estimating bacteria-material interactions. Eur Cell Mater. 2004;8:37–57.
An YH, Friedman RJ. Concise review of mechanisms of bacterial adhesion to biomaterial surfaces. J Biomed Mater Res. 1998;43(3):338–48.
Hallab NJ, Bundy KJ, O’Connor K, Moses RL, Jacobs JJ. Evaluation of metallic and polymeric biomaterial surface energy and surface roughness characteristics for directed cell adhesion. Tissue Eng. 2001;7(1):55–71.
Fischer B, Vaudaux P, Magnin M, el Mestikawy Y, Proctor RA, Lew DP, et al. Novel animal model for studying the molecular mechanisms of bacterial adhesion to bone-implanted metallic devices: role of fibronectin in Staphylococcus aureus adhesion. J Orthop Res. 1996;14(6):914–20.
Acknowledgments
The work is funded by the Spanish Ministry of Education (grant MAT2006-12603-CO2-O2), Comunidad de Madrid (grants S-0505/MAT0324, S2009/MAT-1472), and Ministry of Science (grant CONSOLIDER-INGENIO 2010 CSD 2008-0023 FUNCOAT). DMM was granted by Fundación Conchita Rábago de Jiménez Díaz. The authors thank Dr. J.J. Granizo, epidemiologist, for his help with statistical analysis.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Gómez-Barrena, E., Esteban, J., Molina-Manso, D. et al. Bacterial adherence on UHMWPE with vitamin E: an in vitro study. J Mater Sci: Mater Med 22, 1701–1706 (2011). https://doi.org/10.1007/s10856-011-4340-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10856-011-4340-5