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Assessment of in-vitro bio accessibility and characterization of spray dried complex of astaxanthin with methylated betacyclodextrin

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Abstract

Astaxanthin (AXT) is a carotenoid which gained a lot of importance as a nutritional ingredient in Nutraceuticals and as a food additive. However this bioactive failed to gather absolute significance due to poor aqueous solubility. This study explores the improved dissolution rate of AXT by its complexation with methyl betacyclodextrin (M-βCD) using spray drying technique which is very much scalable and accepted in industry. The experimental methodology undertaken proved that at 1.0 molar concentration of M-βCD, the solubility of AXT was 0.012 mM, representing a 54-fold enhancement over baseline solubility (<0.025 µg/mL). A ten fold increase in the dissolution rate was observed within 45 min in case of spray dried complex of AXT with M-βCD as compared to plain AXT. HepG2 cell line study proved that the bio accessibility of AXT increased when complexed with M-βCD using spray drying technique. Also, these complexes were further characterized by FTIR, UV, DSC, 1H NMR, XRD and molecular modeling analysis. The results confirmed that the hexatomic side rings of the AXT molecule were partly incorporated into the M-βCD cavity. This study provides the basis for the development of soluble and bioavailable oral formulations of AXT using spray drying technology for cyclodextrin complexation.

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Acknowledgments

OmniActive Health Technologies Ltd. (India) are greatly acknowledged for research support and ACTREC (Navi Mumbai) for carrying out cell line study. The authors are also thankful to Rouquette Corporation (Lestrem, France) for providing the free sample of KLEPTOSE® CRYSMEB.

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The authors declare no conflicts of interest.

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Authors and Affiliations

  1. Institute of Pharmacy, Nirma University, Ahmedabad, 382481, India

    Pravin Nalawade

  2. Ramanbhai Patel College of Pharmacy, CHARUSAT, Changa, Petlad, Anand, 388421, India

    Anuradha Gajjar

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  1. Pravin Nalawade
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Correspondence to Pravin Nalawade.

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Nalawade, P., Gajjar, A. Assessment of in-vitro bio accessibility and characterization of spray dried complex of astaxanthin with methylated betacyclodextrin. J Incl Phenom Macrocycl Chem 83, 63–75 (2015). https://doi.org/10.1007/s10847-015-0541-8

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  • DOI: https://doi.org/10.1007/s10847-015-0541-8

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