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2-Methylisoborneol production characteristics of Pseudanabaena sp. FACHB 1277 isolated from Xionghe Reservoir, China

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Abstract

The cyanobacterium Pseudanabaena sp. FACHB 1277, a 2-methylisoborneol (2-MIB) producer isolated from Xionghe Reservoir, was identified by molecular biological methods based on the 16S rDNA sequence. Pseudanabaena sp. FACHB 1277 is a planktonic freshwater species with relatively high 2-MIB per cell density value (7.76 × 10−6 ng cell−1) and specific growth rate (0.25 ± 0.01 d−1). The effects of temperature and light intensity on 2-MIB production of Pseudanabaena sp. FACHB 1277 were investigated. Of the six temperatures tested, 10, 15, 20, 25, 30, and 35 °C, the maximum total 2-MIB per cell density and minimum cell density were observed at 10 °C, while the total 2-MIB and dissolved 2-MIB (including extracellular and dissolved intracellular 2-MIB) increased with increasing temperature. Among the six tested light intensities (10, 25, 40, 55, 70, and 85 μmol photons m−2 s−1), the minimum total 2-MIB per cell density and maximum cell density were observed at 25 μmol photons m−2 s−1. The total 2-MIB and extracellular 2-MIB increased with light intensity increasing from 10 to 40 μmol photons m−2 s−1, while no significant increase was observed when the light intensity was higher than 40 μmol photons m−2 s−1. The maximum intracellular 2-MIB (including dissolved and bound) occurred at 25 μmol photons m−2 s−1. The present study indicates that increasing temperature could favor the conversion of bound intracellular to dissolved 2-MIB, while increasing light intensity stimulates the release of dissolved intracellular 2-MIB into the environment.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China-Israel Science Foundation (Grant No. 41561144008) and the National Natural Science Foundation of China (Grant No. 31000183).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, 410083, China

    Ting Zhang

  2. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, China

    Lingling Zheng, Lin Li & Lirong Song

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  1. Ting Zhang
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  2. Lingling Zheng
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Correspondence to Ting Zhang or Lirong Song.

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Zhang, T., Zheng, L., Li, L. et al. 2-Methylisoborneol production characteristics of Pseudanabaena sp. FACHB 1277 isolated from Xionghe Reservoir, China. J Appl Phycol 28, 3353–3362 (2016). https://doi.org/10.1007/s10811-016-0864-x

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