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Thermokinetic stability of phycocyanin and phycoerythrin in food-grade preservatives

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Abstract

Phycocyanin (PC) and phycoerythrin (PE) are the main phycobiliproteins (PBPs) with application as colorants in food industries. In the present study, the thermokinetic stability of PBPs extracted and purified from a hot spring cyanobacterium, Nostoc sp. strain HKAR-2, was investigated. The individual components of PC and PE were isolated with a high purity ratio of 3.18 (A 615/A 280) and 7.2 (A 563/A 280), respectively. The thermokinetic stability of purified PC and PE was studied in the presence of edible preservatives such as benzoic acid, citric acid, sucrose, ascorbic acid, and calcium chloride over 30 days of incubation at 4, 25, and 40 °C. The rate of degradation (k value) of PC/PE was increased in the control (without preservative), while it declined in the presence of preservatives. The k value (day−1) was found to be the lowest for benzoic acid-treated PC/PE in comparison to the control at 4 °C. The rate of degradation also declined in benzoic acid-treated PC (0.008 day−1) and PE (0.012 day−1) at 40 °C in comparison with the control. Citric acid and sucrose were also found to maintain the stability of both PC and PE at the same temperature. Calcium chloride and ascorbic acid were shown to be the preservatives that support the least stability of PC and PE in comparison to the other preservatives studied. Overall, benzoic acid was found to be the best preservative for both PC and PE at 4 °C.

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References

  • Adams SM, Kao OHW, Berns DS (1979) Psychrophile c-phycocyanin. Plant Physiol 64:525–527

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Antelo FS, Costa JAV, Kalil SJ (2008) Thermal degradation kinetics of the phycocyanin from Spirulina platensis. Biochem Eng J 41:43–47

    Article  CAS  Google Scholar 

  • Bennett A, Bogorad L (1973) Complementary chromatic adaptation in a filamentous blue-green alga. J Cell Biol 58:419–435

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bermejo R, Acién FG, Ibáñez MJ, Fernández JM, Molina E, Alvarez-Pez JM (2003) Preparative purification of B-phycoerythrin from the microalga Porphyridium cruentum by expanded-bed adsorption chromatography. J Chromatogr B 790:317–325

    Article  CAS  Google Scholar 

  • Bhaskar SU, Gopalaswamy G, Raghu R (2005) A simple method for efficient extraction and purification of c-phycocyanin from Spirulina platensis Geitler. Indian J Exp Biol 43:277–279

    CAS  PubMed  Google Scholar 

  • Borowitzka MA (2014) Patents on cyanobacteria and cyanobacterial products and uses. In: Sharma NK, Rai AK, Stal LJ (eds) Cyanobacteria: an economic perspective. Wiley, London, pp 329–338

    Google Scholar 

  • Brul S, Coote P (1999) Preservative agents in foods: mode of action and microbial resistance mechanisms. Int J Food Microbiol 50:1–17

    Article  CAS  PubMed  Google Scholar 

  • Bryant DA, Guglielmi G, Tandeau de Marsac N, Castets AM, Bazire GC (1979) The structure of cyanobacterial phycobilisomes: a model. Arch Microbiol 123:113–127

    Article  CAS  Google Scholar 

  • Chaiklahan R, Chirasuwana N, Bunnag B (2012) Stability of phycocyanin extracted from Spirulina sp.: influence of temperature, pH and preservatives. Process Biochem 47:659–664

    Article  CAS  Google Scholar 

  • Dong C, Wang W (2006) Headspace solid-phase micro extraction applied to the simultaneous determination of sorbic and benzoic acids in beverages. Anal Chim Acta 562:23–29

    Article  CAS  Google Scholar 

  • Grossman A, Schaefer MR, Chiang GG, Collier JL (1993) The phycobilisomes, a light-harvesting complex responsive to environmental conditions. Microbiol Rev 57:725–749

    CAS  PubMed  PubMed Central  Google Scholar 

  • Kannaujiya VK, Sinha RP (2015) Impacts of varying light regimes on phycobiliproteins of Nostoc sp. HKAR-2 and Nostoc sp. HKAR-11 isolated from diverse habitats. Protoplasma. doi:10.1007/s00709-015-0786-5

    PubMed  Google Scholar 

  • Kao OHW, Edwards MR, Berns DS (1975) Physical-chemical properties of c-phycocyanin isolated from an acido-thermophilic eukaryote, Cyanidium caldarium. Biochem J 147:63–70

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Koca N, Karadeniz F, Burdurlu HS (2006) Effect of pH on chlorophyll degradation and colour loss in blanched green peas. Food Chem 100:609–615

    Article  Google Scholar 

  • Leema JT, Kirubagaran R, Vinithkumar NV, Dheenan PS, Karthikayulu S (2010) High value pigment production from Arthrospira (Spirulina) platensis cultured in seawater. Bioresour Technol 101:9221–9227

    Article  PubMed  Google Scholar 

  • Lino CM, Pena A (2010) Occurrence of caffeine, saccharin, benzoic acid and sorbic acid in soft drinks and nectars in Portugal and subsequent exposure assessment. Food Chem 121:503–508

    Article  CAS  Google Scholar 

  • Martelli G, Folli C, Visai L, Daglia M, Ferrari D (2014) Thermal stability improvement of blue colorant c-phycocyanin from Spirulina platensis for food industry applications. Process Biochem 49:154–159

    Article  CAS  Google Scholar 

  • Mishra SK, Shrivastav A, Mishra S (2008) Effect of preservatives for food grade PC from Spirulina platensis. Process Biochem 43:339–345

    Article  CAS  Google Scholar 

  • Mishra SK, Shrivastav A, Pancha I, Jain D, Mishra S (2010) Effect of preservatives for food grade c-phycoerythrin, isolated from marine cyanobacteria Pseudanabaena sp. Int J Biol Macromol 47:597–602

    Article  CAS  PubMed  Google Scholar 

  • Oh S, Shin M, Lee K, Choe E (2013) Effects of water activity on pigments in dried laver (Porphyra) during storage. Food Sci Biotechnol 22:1523–1529

    Article  CAS  Google Scholar 

  • Padyana AK, Bhat VB, Madyastha KM, Rajashankar KR, Ramakumar S (2001) Crystal structure of a light-harvesting protein C-phycocyanin from Spirulina platensis. Biochem Biophys Res Commun 282:893–898

    Article  CAS  PubMed  Google Scholar 

  • Patel A, Pawar R, Mishra S, Sonawane S, Ghosh PK (2004) Kinetic studies on thermal denaturation of C-phycocyanin. Indian J Biochem Biophys 41:254–257

    CAS  PubMed  Google Scholar 

  • Pleonsil P, Soogarun S, Suwanwong Y (2013) Anti-oxidant activity of holo- and apo-c-phycocyanin and their protective effects on human erythrocytes. Int J Biol Macromol 60:393–398

    Article  CAS  PubMed  Google Scholar 

  • Rastogi RP, Kumari S, Richa HT, Sinha RP (2012) Molecular characterization of hot spring cyanobacteria and evaluation of their photoprotective compounds. Can J Microbiol 58:719–727

    Article  CAS  PubMed  Google Scholar 

  • Richa, Kannaujiya VK, Kesheri M, Singh G, Sinha RP (2011) Biotechnological potentials of phycobiliproteins. Int J Pharma Biosci 2:446–454

    CAS  Google Scholar 

  • Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61

    Google Scholar 

  • Santiago-Santos MC, Ponce-Noyola T, Olvera-Ramírez R, Ortega-Lόpez J, Cañizares-Villanueva RO (2000) Extraction and purification of phycocyanin from Calothrix sp. Process Biochem 39:2047–2052

    Article  Google Scholar 

  • Sarada R, Pillai MG, Ravishankar GA (1999) Phycocyanin from Spirulina sp.: influence of processing of biomass on phycocyanin yield, analysis of efficacy of extraction methods and stability studies on phycocyanin. Process Biochem 34:795–801

    Article  CAS  Google Scholar 

  • Sekar S, Chandramohan M (2008) Phycobiliproteins as a commodity: trends in applied research, patents and commercialization. J Appl Phycol 20:113–136

    Article  Google Scholar 

  • Shanab SM, Mostafa SS, Shalaby EA, Mahmoud GI (2012) Aqueous extracts of microalgae exhibit antioxidant and anticancer activities. Asian Pac J Trop Biomed 2:608–615

    Article  PubMed  PubMed Central  Google Scholar 

  • Sinha RP, Häder D-P (2003) Biochemistry of phycobilisomes disassembly by ultraviolet-B radiation in cyanobacteria. Recent Res Devel Biochem 4:945–955

    CAS  Google Scholar 

  • Spolaore P, Joannis-Cassan C, Duran E, Isambert A (2006) Commercial applications of microalgae. J Biosci Bioeng 101:87–96

    Article  CAS  PubMed  Google Scholar 

  • Sun L, Shumei W, Chen L, Gong X (2003) Promising fluorescent probes from phycobiliproteins. IEEE J Sel Top Quantum Electron 9:177–188

    Article  CAS  Google Scholar 

  • Zheng J, Inoguchi T, Sasaki S, Maeda Y, McCarty MF, Fujii M, Ikeda N, Kobayashi K, Sonoda N, Takayanagi R (2013) Phycocyanin and phycocyanobilin from Spirulina platensis protect against diabetic nephropathy by inhibiting oxidative stress. Am J Physiol Regul Integr Comp Physiol 304:110–120

    Article  Google Scholar 

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Acknowledgments

V. K. Kannaujiya is thankful to the Council of Scientific and Industrial Research (CSIR), New Delhi, India (09/013(0258)/2009-EMR-I), for the financial assistance in the form of a senior research fellowship. Thanks are also due to Prof. Dr. D-P. Häder for critically reading the manuscript.

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  1. Laboratory of Photobiology and Molecular Microbiology, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221005, India

    Vinod K. Kannaujiya & Rajeshwar P. Sinha

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  1. Vinod K. Kannaujiya
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  2. Rajeshwar P. Sinha
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Correspondence to Rajeshwar P. Sinha.

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Kannaujiya, V.K., Sinha, R.P. Thermokinetic stability of phycocyanin and phycoerythrin in food-grade preservatives. J Appl Phycol 28, 1063–1070 (2016). https://doi.org/10.1007/s10811-015-0638-x

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  • DOI: https://doi.org/10.1007/s10811-015-0638-x

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