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The effects of ultraviolet radiation-B response on the morphology, ultrastructure, and photosynthetic pigments of Laurencia catarinensis and Palisada flagellifera (Ceramiales, Rhodophyta): a comparative study

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Abstract

The present work aims to compare the effects of ultraviolet radiation on the morphology, ultrastructure, and photosynthetic pigments of two species of Ceramiales: Laurencia catarinensis and Palisada flagellifera. To accomplish this, plants were cultivated and exposed to photosynthetically active radiation (PAR) at 60 μmol photons m−2 s−1 and PAR + ultraviolet B radiation (UVBR) at 0.35 W m−2 at 3 h per day for 7 days. Subsequently, the apical segments of L. catarinensis and P. flagellifera were analyzed under light and transmission microscopy, and both growth rates and photosynthetic pigments were studied. After exposure to PAR + UVBR, L. catarinensis exhibited a high reduction in growth rate and loss of biomass, in addition to a reduction in the concentration of chlorophyll a when compared with treated plants of P. flagellifera. However, after 7 days of exposure to PAR + UVBR, both plants showed a reduction in phycobiliprotein content. Toluidine blue reaction did not show cell wall changes in treated species with PAR + UVBR. Staining of L. catarinensis and P. flagellifera with periodic acid–Schiff (PAS) after UVBR exposure showed an increase in the number of starch grains in both species. When observed by transmission electron microscopy, treated samples of both species also showed disrupted thylakoids of the chloroplasts and an increased number of plastoglobuli. Based on this line of evidence, the present study demonstrates that ultraviolet radiation negatively affects the intertidal macroalgae L. catarinensis and P. flagellifera.

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Acknowledgments

The authors would like to acknowledge the staff of the Central Laboratory of Electron Microscopy (LCME), Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil, for the use of their transmission electron microscope. Éder C. Schmidt holds a postdoctoral fellowship from CAPES. Zenilda L. Bouzon is a CNPq fellow.

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Authors and Affiliations

  1. Scientific Initiation-PIBIC-CNPq, Plant Cell Biology Laboratory, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina 88049-900, CP 476, Florianópolis, SC, Brazil

    Debora T. Pereira

  2. Postdoctoral Research of Post-Graduate Program in Cell Biology and Development, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina 88049-900, CP 476, Florianópolis, SC, Brazil

    Éder C. Schmidt

  3. Central Laboratory of Electron Microscopy, Federal University of Santa Catarina 88049-900, CP 476, Florianópolis, SC, Brazil

    Zenilda L. Bouzon

  4. Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina 88049-900, CP 476, Florianópolis, SC, Brazil

    Luciane C. Ouriques

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  1. Debora T. Pereira
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Correspondence to Éder C. Schmidt.

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Pereira, D.T., Schmidt, É.C., Bouzon, Z.L. et al. The effects of ultraviolet radiation-B response on the morphology, ultrastructure, and photosynthetic pigments of Laurencia catarinensis and Palisada flagellifera (Ceramiales, Rhodophyta): a comparative study. J Appl Phycol 26, 2443–2452 (2014). https://doi.org/10.1007/s10811-014-0262-1

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