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Epidermal Growth Factor Promotes Proliferation and Improves Restoration After Intestinal Ischemia–Reperfusion Injury in Rats

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Abstract

Epidermal growth factor (EGF) is an attractive and promising therapeutic application for intestinal disorders. The current study examined its influence on proliferation and restoration after ischemia–reperfusion (I/R) injury in rat small intestine. Six groups were performed: sham operation (Con); ischemia for 30 min with subsequent reperfusion for 30 min (I/R); I/R injured with 500 μg/kg EGF injected 5 min before ischemia (Pre-l); I/R injured with 50 μg/kg EGF injected 5 min before ischemia (Pre-s); I/R injured with 500 μg/kg EGF injected 5 min after reperfusion (Post-l); and I/R injured with 50 μg/kg EGF injected 5 min after reperfusion (Post-s). Intestinal histological damage, crypt cell proliferation degree, mucosal permeability, tight junction proteins expression, and levels of inflammation factors were studied for each group. Compared with the I/R group, administration of EGF in the Pre-l, Pre-s, and Post-l groups all presented a significant proliferation effect. The levels of FD4, IL-6, and TNF-α were dramatically decreased in all EGF-treated groups. Histological destruction was improved and TJs recovery was notably accelerated in all EGF-treated groups except the Post-s group. d-lactate concentration was only diminished in the Pre-l group. These results suggest that mucosally applied EGF can promote intestinal proliferation and improve restoration after I/R injury. EGF intraluminal administration is an effective treatment against intestinal I/R injury.

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Acknowledgments

This work was supported by the Key Project of National Natural Science Foundation in China (30830098), National Basic Research Program (973 Program) in China (no. 2009CB522405), National Natural Science Foundation in China (81070375), and Scientific Research Fund in Jiangsu Province (BK2009317).

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Authors and Affiliations

  1. Department of Intensive Care Unit, Jiangsu Province Chinese Medicine Hospital, 155 Han Zhong Road, Nanjing, 210029, China

    Yanxia Geng

  2. Department of Surgery, Jinling Hospital, 305 Zhong Shan East Road, Nanjing, 210002, China

    Jieshou Li, Qiurong Li & Weiqin Li

  3. The Affiliated Drum Tower Hospital of Medical School of Nanjing University, Nanjing, 210008, China

    Fan Wang

  4. Department of Intensive Care Unit, Jiangsu Province Chinese Medicine Hospital, Nanjing, 210029, China

    Xing Wang

  5. Department of Intensive Care Unit, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210011, China

    Liqun Sun

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  1. Yanxia Geng
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  2. Jieshou Li
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  3. Fan Wang
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  4. Qiurong Li
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Correspondence to Jieshou Li.

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Geng, Y., Li, J., Wang, F. et al. Epidermal Growth Factor Promotes Proliferation and Improves Restoration After Intestinal Ischemia–Reperfusion Injury in Rats. Inflammation 36, 670–679 (2013). https://doi.org/10.1007/s10753-012-9591-x

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  • DOI: https://doi.org/10.1007/s10753-012-9591-x

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